Q49W03 · ENO_STAS1
- ProteinEnolase
- Geneeno
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids434 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the reversible conversion of 2-phosphoglycerate (2-PG) into phosphoenolpyruvate (PEP). It is essential for the degradation of carbohydrates via glycolysis.
Catalytic activity
- (2R)-2-phosphoglycerate = phosphoenolpyruvate + H2O
Cofactor
Note: Binds a second Mg2+ ion via substrate during catalysis.
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 165 | (2R)-2-phosphoglycerate (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Active site | 207 | Proton donor | ||||
Sequence: E | ||||||
Binding site | 244 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 291 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 318 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Active site | 343 | Proton acceptor | ||||
Sequence: K | ||||||
Binding site | 343 | (2R)-2-phosphoglycerate (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 372 | (2R)-2-phosphoglycerate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 373 | (2R)-2-phosphoglycerate (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 394 | (2R)-2-phosphoglycerate (UniProtKB | ChEBI) | ||||
Sequence: K |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cell surface | |
Cellular Component | extracellular region | |
Cellular Component | phosphopyruvate hydratase complex | |
Molecular Function | magnesium ion binding | |
Molecular Function | phosphopyruvate hydratase activity | |
Biological Process | glycolytic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameEnolase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Staphylococcaceae > Staphylococcus
Accessions
- Primary accessionQ49W03
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Fractions of enolase are present in both the cytoplasm and on the cell surface.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000267114 | 1-434 | Enolase | |||
Sequence: MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTGEHEAVELRDGDKSRYLGKGVTKAVDNVNEIIAPELIEGEFSVLEQVSIDKMMIQLDGTENKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDAPIAFQEFMVLPVGAESFKESLRWGAEIFHNLKSILKNRGLETAVGDEGGFAPKFEGTEDAVETILEAIKAVGLEPGKDVFLGFDCASSEFFEDGVYNYAKFEGENGAKRSAEEQVDYLEELVNKYPIITIEDGMDENDWDGWKVLTDRIGDKVQLVGDDLFVTNTVKLSEGIEKGIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTISDIAVATNAGQIKTGSLSRTDRIAKYNQLLRIEDELYETGKFDGLKSFYNLSK |
Structure
Sequence
- Sequence statusComplete
- Length434
- Mass (Da)47,075
- Last updated2005-09-13 v1
- Checksum45F268DEA24FFCBE
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AP008934 EMBL· GenBank· DDBJ | BAE19057.1 EMBL· GenBank· DDBJ | Genomic DNA |