Q09517 · LE767_CAEEL

  • Protein
    Very-long-chain 3-oxooacyl-coA reductase let-767
  • Gene
    let-767
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Required for branched-chain fatty acid synthesis (such as (omega-1)-methyl-fatty acids) (PubMed:18390550).
Catalyzes the reduction of the 3-keto-fatty acyl-CoA intermediate that is formed in each cycle of fatty acid elongation (PubMed:18390550).
Very long-chain fatty acids (VLCFAs) serve as precursors for ceramide and sphingolipids (PubMed:12905072, PubMed:18390550, PubMed:21926990).
Involved in hormone production as it metabolizes 4-androstendione (androst-4-ene-3,17-dione) into testosterone and estrone into estradiol (17beta-estradiol) in vitro, but the physiological steroid substrate is unknown (PubMed:17951538).

Catalytic activity

Pathway

Lipid metabolism; fatty acid biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site47-76NADP+ (UniProtKB | ChEBI)
Binding site106NADP+ (UniProtKB | ChEBI)
Binding site189substrate
Active site202Proton acceptor
Binding site206NADP+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentapical cortex
Cellular Componentendoplasmic reticulum
Molecular Function17-beta-hydroxysteroid dehydrogenase (NADP+) activity
Molecular Functionlong-chain-fatty-acyl-CoA reductase activity
Molecular Functiontestosterone dehydrogenase [NAD(P)+] activity
Molecular Functionvery-long-chain 3-oxoacyl-CoA reductase activity
Biological Processandrogen metabolic process
Biological Processdevelopmental process
Biological Processestablishment or maintenance of epithelial cell apical/basal polarity
Biological Processestrogen metabolic process
Biological Processfatty acid elongation
Biological Processhormone metabolic process
Biological Processlong-chain fatty acid biosynthetic process
Biological Processmolting cycle, collagen and cuticulin-based cuticle
Biological Processsteroid biosynthetic process

Keywords

Enzyme and pathway databases

Chemistry

Names & Taxonomy

Protein names

  • Recommended name
    Very-long-chain 3-oxooacyl-coA reductase let-767
  • EC number
  • Short names
    LET-767
  • Alternative names
    • Lethal protein 767
    • Short-chain dehydrogenase 10
    • Steroid dehydrogenase let-767 (EC:1.1.1.-
      ) . EC:1.1.1.- (UniProtKB | ENZYME | Rhea)
    • Steroid-dehydrogenase/3-ketoacyl-CoA-reductase

Gene names

    • Name
      let-767
    • Synonyms
      dhs-10
    • ORF names
      C56G2.6

Organism names

  • Taxonomic identifier
  • Strain
    • Bristol N2
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis

Accessions

  • Primary accession
    Q09517
  • Secondary accessions
    • C1P622
    • S6FCZ1

Proteomes

Organism-specific databases

Subcellular Location

Phenotypes & Variants

Disruption phenotype

Worms exhibit slow and retarded growth, reproductive and molting defects, defects in embryogenesis, and hypersensitivity to cholesterol limitation (PubMed:12905072).
Increased polarity and tubulogenesis defects in an allelic series of let-767 (PubMed:21926990).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis135Arrests at early larval stage.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000545781-316Very-long-chain 3-oxooacyl-coA reductase let-767

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in the gut of larva and adult.

Developmental stage

Expressed from first larval stage to adult.

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence & Isoforms

Align isoforms (3)
  • Sequence status
    Complete

This entry describes 3 isoforms produced by Alternative initiation.

Q09517-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    316
  • Mass (Da)
    34,309
  • Last updated
    2002-06-20 v2
  • Checksum
    DA3C6377AC4C12CE
MACQCFLVGAGYVALAAVAYRLLTIFSNILGPYVLLSPIDLKKRAGASWAVVTGATDGIGKAYAFELARRGFNVLLVSRTQSKLDETKKEILEKYSSIEVRTAAFDFTNAAPSAYKDLLATLNQVEIGVLINNVGMSYEYPDVLHKVDGGIERLANITTINTLPPTLLSAGILPQMVARKAGVIVNVGSSAGANQMALWAVYSATKKYVSWLTAILRKEYEHQGITVQTIAPMMVATKMSKVKRTSFFTPDGAVFAKSALNTVGNTSDTTGYITHQLQLELMDLIPTFIRDKILTNMSVGTRAAALRKKEREAKSQ

Q09517-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 1-1: M → MESSDNLHDIDNLENGNM

Q09517-3

  • Name
    3
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_0610631in isoform 2 and isoform 3
Alternative sequenceVSP_061064167in isoform 3

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FO080744
EMBL· GenBank· DDBJ
CCD66337.1
EMBL· GenBank· DDBJ
Genomic DNA
BX284603
EMBL· GenBank· DDBJ
CCD66339.1
EMBL· GenBank· DDBJ
Genomic DNA
BX284603
EMBL· GenBank· DDBJ
CDG24102.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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