Q09517 · LE767_CAEEL
- ProteinVery-long-chain 3-oxooacyl-coA reductase let-767
- Genelet-767
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids316 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Required for branched-chain fatty acid synthesis (such as (omega-1)-methyl-fatty acids) (PubMed:18390550).
Catalyzes the reduction of the 3-keto-fatty acyl-CoA intermediate that is formed in each cycle of fatty acid elongation (PubMed:18390550).
Very long-chain fatty acids (VLCFAs) serve as precursors for ceramide and sphingolipids (PubMed:12905072, PubMed:18390550, PubMed:21926990).
Involved in hormone production as it metabolizes 4-androstendione (androst-4-ene-3,17-dione) into testosterone and estrone into estradiol (17beta-estradiol) in vitro, but the physiological steroid substrate is unknown (PubMed:17951538).
Catalyzes the reduction of the 3-keto-fatty acyl-CoA intermediate that is formed in each cycle of fatty acid elongation (PubMed:18390550).
Very long-chain fatty acids (VLCFAs) serve as precursors for ceramide and sphingolipids (PubMed:12905072, PubMed:18390550, PubMed:21926990).
Involved in hormone production as it metabolizes 4-androstendione (androst-4-ene-3,17-dione) into testosterone and estrone into estradiol (17beta-estradiol) in vitro, but the physiological steroid substrate is unknown (PubMed:17951538).
Catalytic activity
- a very-long-chain (3R)-3-hydroxyacyl-CoA + NADP+ = a very-long-chain 3-oxoacyl-CoA + NADPH + H+This reaction proceeds in the backward direction.
- (omega-1)-methyl-(3R)-hydroxy-fatty acyl-CoA + NADP+ = (omega-1)-methyl-3-oxo-fatty acyl-CoA + NADPH + H+This reaction proceeds in the backward direction.
- a 17beta-hydroxy steroid + NADP+ = a 17-oxo steroid + NADPH + H+This reaction proceeds in the backward direction.
Pathway
Lipid metabolism; fatty acid biosynthesis.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | apical cortex | |
Cellular Component | endoplasmic reticulum | |
Molecular Function | 17-beta-hydroxysteroid dehydrogenase (NADP+) activity | |
Molecular Function | long-chain-fatty-acyl-CoA reductase activity | |
Molecular Function | testosterone dehydrogenase [NAD(P)+] activity | |
Molecular Function | very-long-chain 3-oxoacyl-CoA reductase activity | |
Biological Process | androgen metabolic process | |
Biological Process | developmental process | |
Biological Process | establishment or maintenance of epithelial cell apical/basal polarity | |
Biological Process | estrogen metabolic process | |
Biological Process | fatty acid elongation | |
Biological Process | hormone metabolic process | |
Biological Process | long-chain fatty acid biosynthetic process | |
Biological Process | molting cycle, collagen and cuticulin-based cuticle | |
Biological Process | steroid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Chemistry
Names & Taxonomy
Protein names
- Recommended nameVery-long-chain 3-oxooacyl-coA reductase let-767
- EC number
- Short namesLET-767
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionQ09517
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
Disruption phenotype
Worms exhibit slow and retarded growth, reproductive and molting defects, defects in embryogenesis, and hypersensitivity to cholesterol limitation (PubMed:12905072).
Increased polarity and tubulogenesis defects in an allelic series of let-767 (PubMed:21926990).
Increased polarity and tubulogenesis defects in an allelic series of let-767 (PubMed:21926990).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 135 | Arrests at early larval stage. | ||||
Sequence: G → R |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000054578 | 1-316 | Very-long-chain 3-oxooacyl-coA reductase let-767 | |||
Sequence: MACQCFLVGAGYVALAAVAYRLLTIFSNILGPYVLLSPIDLKKRAGASWAVVTGATDGIGKAYAFELARRGFNVLLVSRTQSKLDETKKEILEKYSSIEVRTAAFDFTNAAPSAYKDLLATLNQVEIGVLINNVGMSYEYPDVLHKVDGGIERLANITTINTLPPTLLSAGILPQMVARKAGVIVNVGSSAGANQMALWAVYSATKKYVSWLTAILRKEYEHQGITVQTIAPMMVATKMSKVKRTSFFTPDGAVFAKSALNTVGNTSDTTGYITHQLQLELMDLIPTFIRDKILTNMSVGTRAAALRKKEREAKSQ |
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in the gut of larva and adult.
Developmental stage
Expressed from first larval stage to adult.
Gene expression databases
Structure
Sequence & Isoforms
- Sequence statusComplete
This entry describes 3 isoforms produced by Alternative initiation.
Q09517-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length316
- Mass (Da)34,309
- Last updated2002-06-20 v2
- ChecksumDA3C6377AC4C12CE
Q09517-2
- Name2
- Differences from canonical
- 1-1: M → MESSDNLHDIDNLENGNM
Q09517-3
- Name3
Features
Showing features for alternative sequence.
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FO080744 EMBL· GenBank· DDBJ | CCD66337.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284603 EMBL· GenBank· DDBJ | CCD66339.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284603 EMBL· GenBank· DDBJ | CDG24102.1 EMBL· GenBank· DDBJ | Genomic DNA |