UniProtKB - P9WQJ9 (IRTA_MYCTU)
Protein
Mycobactin import ATP-binding/permease protein IrtA
Gene
irtA
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Part of the ABC transporter complex IrtAB involved in the import of iron-bound mycobactin (Fe-MBT) and carboxymycobactin (Fe-cMBT) (PubMed:16385031, PubMed:19948799) (By similarity). Mycobactins are then reduced by the siderophore interaction domain to facilitate iron release in the bacterial cell (By similarity). Transmembrane domains (TMD) form a pore in the membrane and the ATP-binding domain (NBD) is responsible for energy generation (By similarity). Required for replication in human macrophages and in mouse lungs (PubMed:16385031).By similarity2 Publications
Caution
PubMed:18461140 reports that IrtA is a siderophore exporter, however this activity could be due to functional differences of IrtA in the molecular context of M.smegmatis and M.tuberculosis.Curated
Cofactori
FAD1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 70 – 73 | FADBy similarity | 4 | |
Nucleotide bindingi | 87 – 91 | FADBy similarity | 5 | |
Nucleotide bindingi | 97 – 98 | FADBy similarity | 2 | |
Nucleotide bindingi | 643 – 650 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATPase activity Source: InterPro
- ATPase-coupled transmembrane transporter activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- FAD binding Source: MTBBASE
- oxidoreductase activity Source: InterPro
- siderophore transmembrane transporter activity Source: MTBBASE
GO - Biological processi
- cellular response to iron ion starvation Source: MTBBASE
- iron acquisition from host Source: MTBBASE
- iron ion homeostasis Source: MTBBASE
- pathogenesis Source: MTBBASE
- response to host iron concentration Source: MTBBASE
- siderophore-dependent iron import into cell Source: GO_Central
Keywordsi
Molecular function | Translocase |
Biological process | Transport |
Ligand | ATP-binding, FAD, Flavoprotein, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-HSA-1222449, Mtb iron assimilation by chelation |
Names & Taxonomyi
Protein namesi | Recommended name: Mycobactin import ATP-binding/permease protein IrtACurated (EC:7.2.2.-)Alternative name(s): Iron-regulated transporter A |
Gene namesi | Name:irtA Ordered Locus Names:Rv1348 ORF Names:MTCY02B10.12 |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv1348 |
Subcellular locationi
- Cell inner membrane 1 Publication; Multi-pass membrane protein PROSITE-ProRule annotation1 Publication
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 292 | Cytoplasmic1 PublicationAdd BLAST | 292 | |
Transmembranei | 293 – 313 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 314 – 334 | Periplasmic1 PublicationAdd BLAST | 21 | |
Transmembranei | 335 – 355 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 356 – 408 | Cytoplasmic1 PublicationAdd BLAST | 53 | |
Transmembranei | 409 – 429 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 430 – 432 | Periplasmic1 Publication | 3 | |
Transmembranei | 433 – 453 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 454 – 519 | Cytoplasmic1 PublicationAdd BLAST | 66 | |
Transmembranei | 520 – 540 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 541 – 548 | Periplasmic1 Publication | 8 | |
Transmembranei | 549 – 569 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Topological domaini | 570 – 859 | Cytoplasmic1 PublicationAdd BLAST | 290 |
GO - Cellular componenti
- integral component of plasma membrane Source: GO_Central
- plasma membrane Source: MTBBASE
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 70 | R → A: No change in FAD-binding and in activity. 1 Publication | 1 | |
Mutagenesisi | 72 | Y → A: Decrease in FAD-binding and loss of activity. 1 Publication | 1 | |
Mutagenesisi | 73 | T → A: Decrease in FAD-binding and loss of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000093255 | 1 – 859 | Mycobactin import ATP-binding/permease protein IrtAAdd BLAST | 859 |
Proteomic databases
PaxDbi | P9WQJ9 |
Expressioni
Inductioni
Repressed by iron and IdeR.2 Publications
Interactioni
Subunit structurei
Forms a heterodimer with IrtB.
2 PublicationsProtein-protein interaction databases
STRINGi | 83332.Rv1348 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 15 – 122 | FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST | 108 | |
Domaini | 293 – 575 | ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST | 283 | |
Domaini | 610 – 843 | ABC transporterPROSITE-ProRule annotationAdd BLAST | 234 |
Domaini
In IrtA the ATP-binding domain (NBD) and the transmembrane domain (TMD) are fused (PubMed:16385031). In addition, IrtA contains an N-terminal siderophore interaction domain (SID) that binds FAD (PubMed:19948799).2 Publications
Sequence similaritiesi
Belongs to the ABC transporter superfamily. Siderophore-Fe(3+) uptake transporter (SIUT) (TC 3.A.1.21) family. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1132, Bacteria COG2375, Bacteria |
OMAi | GAYLEGQ |
PhylomeDBi | P9WQJ9 |
Family and domain databases
Gene3Di | 1.20.1560.10, 1 hit 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR013113, FAD-bd_9_SIP IPR017927, FAD-bd_FR_type IPR039261, FNR_nucleotide-bd IPR027417, P-loop_NTPase IPR017938, Riboflavin_synthase-like_b-brl IPR007037, SIP_C |
Pfami | View protein in Pfam PF00664, ABC_membrane, 1 hit PF00005, ABC_tran, 1 hit PF08021, FAD_binding_9, 1 hit PF04954, SIP, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF63380, SSF63380, 1 hit SSF90123, SSF90123, 1 hit |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 1 hit PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit PS51384, FAD_FR, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WQJ9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MARGLQGVML RSFGARDHTA TVIETISIAP HFVRVRMVSP TLFQDAEAEP
60 70 80 90 100
AAWLRFWFPD PNGSNTEFQR AYTISEADPA AGRFAVDVVL HDPAGPASSW
110 120 130 140 150
ARTVKPGATI AVMSLMGSSR FDVPEEQPAG YLLIGDSASI PGMNGIIETV
160 170 180 190 200
PNDVPIEMYL EQHDDNDTLI PLAKHPRLRV RWVMRRDEKS LAEAIENRDW
210 220 230 240 250
SDWYAWATPE AAALKCVRVR LRDEFGFPKS EIHAQAYWNA GRAMGTHRAT
260 270 280 290 300
EPAATEPEVG AAPQPESAVP APARGSWRAQ AASRLLAPLK LPLVLSGVLA
310 320 330 340 350
ALVTLAQLAP FVLLVELSRL LVSGAGAHRL FTVGFAAVGL LGTGALLAAA
360 370 380 390 400
LTLWLHVIDA RFARALRLRL LSKLSRLPLG WFTSRGSGSI KKLVTDDTLA
410 420 430 440 450
LHYLVTHAVP DAVAAVVAPV GVLVYLFVVD WRVALVLFGP VLVYLTITSS
460 470 480 490 500
LTIQSGPRIV QAQRWAEKMN GEAGSYLEGQ PVIRVFGAAS SSFRRRLDEY
510 520 530 540 550
IGFLVAWQRP LAGKKTLMDL ATRPATFLWL IAATGTLLVA THRMDPVNLL
560 570 580 590 600
PFMFLGTTFG ARLLGIAYGL GGLRTGLLAA RHLQVTLDET ELAVREHPRE
610 620 630 640 650
PLDGEAPATV VFDHVTFGYR PGVPVIQDVS LTLRPGTVTA LVGPSGSGKS
660 670 680 690 700
TLATLLARFH DVERGAIRVG GQDIRSLAAD ELYTRVGFVL QEAQLVHGTA
710 720 730 740 750
AENIALAVPD APAEQVQVAA REAQIHDRVL RLPDGYDTVL GANSGLSGGE
760 770 780 790 800
RQRLTIARAI LGDTPVLILD EATAFADPES EYLVQQALNR LTRDRTVLVI
810 820 830 840 850
AHRLHTITRA DQIVVLDHGR IVERGTHEEL LAAGGRYCRL WDTGQGSRVA
VAAAQDGTR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP44106.1 |
PIRi | C70740 |
RefSeqi | NP_215864.1, NC_000962.3 WP_003406958.1, NZ_NVQJ01000031.1 |
Genome annotation databases
EnsemblBacteriai | CCP44106; CCP44106; Rv1348 |
GeneIDi | 23492497 886853 |
KEGGi | mtu:Rv1348 mtv:RVBD_1348 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP44106.1 |
PIRi | C70740 |
RefSeqi | NP_215864.1, NC_000962.3 WP_003406958.1, NZ_NVQJ01000031.1 |
3D structure databases
SMRi | P9WQJ9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv1348 |
Proteomic databases
PaxDbi | P9WQJ9 |
Genome annotation databases
EnsemblBacteriai | CCP44106; CCP44106; Rv1348 |
GeneIDi | 23492497 886853 |
KEGGi | mtu:Rv1348 mtv:RVBD_1348 |
Organism-specific databases
TubercuListi | Rv1348 |
Phylogenomic databases
eggNOGi | COG1132, Bacteria COG2375, Bacteria |
OMAi | GAYLEGQ |
PhylomeDBi | P9WQJ9 |
Enzyme and pathway databases
Reactomei | R-HSA-1222449, Mtb iron assimilation by chelation |
Family and domain databases
Gene3Di | 1.20.1560.10, 1 hit 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011527, ABC1_TM_dom IPR036640, ABC1_TM_sf IPR003439, ABC_transporter-like IPR017871, ABC_transporter_CS IPR013113, FAD-bd_9_SIP IPR017927, FAD-bd_FR_type IPR039261, FNR_nucleotide-bd IPR027417, P-loop_NTPase IPR017938, Riboflavin_synthase-like_b-brl IPR007037, SIP_C |
Pfami | View protein in Pfam PF00664, ABC_membrane, 1 hit PF00005, ABC_tran, 1 hit PF08021, FAD_binding_9, 1 hit PF04954, SIP, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF63380, SSF63380, 1 hit SSF90123, SSF90123, 1 hit |
PROSITEi | View protein in PROSITE PS50929, ABC_TM1F, 1 hit PS00211, ABC_TRANSPORTER_1, 1 hit PS50893, ABC_TRANSPORTER_2, 1 hit PS51384, FAD_FR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IRTA_MYCTU | |
Accessioni | P9WQJ9Primary (citable) accession number: P9WQJ9 Secondary accession number(s): L0T6D6, P63391, Q11018 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | December 2, 2020 | |
This is version 44 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families