UniProtKB - P9WQF5 (LEUC_MYCTU)
Protein
3-isopropylmalate dehydratase large subunit
Gene
leuC
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation
Catalytic activityi
- EC:4.2.1.33UniRule annotation
Cofactori
[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation
: L-leucine biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.UniRule annotationProteins known to be involved in the 4 steps of the subpathway in this organism are:
- 2-isopropylmalate synthase (leuA)
- 3-isopropylmalate dehydratase large subunit (leuC), 3-isopropylmalate dehydratase small subunit (leuD)
- 3-isopropylmalate dehydrogenase (leuB)
- Branched-chain-amino-acid aminotransferase (ilvE)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 354 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 414 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 417 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 |
GO - Molecular functioni
- 3-isopropylmalate dehydratase activity Source: UniProtKB-UniRule
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- leucine biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Lyase |
Biological process | Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-7243-MONOMER |
UniPathwayi | UPA00048;UER00071 |
Names & Taxonomyi
Protein namesi | Recommended name: 3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)Alternative name(s): Alpha-IPM isomeraseUniRule annotation Short name: IPMIUniRule annotation Isopropylmalate isomeraseUniRule annotation |
Gene namesi | Name:leuCUniRule annotation Ordered Locus Names:Rv2988c ORF Names:MTV012.02c |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv2988c |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000076766 | 1 – 473 | 3-isopropylmalate dehydratase large subunitAdd BLAST | 473 |
Proteomic databases
PaxDbi | P9WQF5 |
PRIDEi | P9WQF5 |
Interactioni
Subunit structurei
Heterodimer of LeuC and LeuD.
UniRule annotationProtein-protein interaction databases
STRINGi | 83332.Rv2988c |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG4105CQI Bacteria COG0065 LUCA |
KOi | K01703 |
OMAi | CNMSIEM |
PhylomeDBi | P9WQF5 |
Family and domain databases
CDDi | cd01583 IPMI, 1 hit |
Gene3Di | 3.30.499.10, 1 hit |
HAMAPi | MF_01026 LeuC_type1, 1 hit |
InterProi | View protein in InterPro IPR004430 3-IsopropMal_deHydase_lsu IPR015931 Acnase/IPM_dHydase_lsu_aba_1/3 IPR001030 Acoase/IPM_deHydtase_lsu_aba IPR018136 Aconitase_4Fe-4S_BS IPR036008 Aconitase_4Fe-4S_dom IPR033941 IPMI_cat |
Pfami | View protein in Pfam PF00330 Aconitase, 1 hit |
PRINTSi | PR00415 ACONITASE |
SUPFAMi | SSF53732 SSF53732, 1 hit |
TIGRFAMsi | TIGR00170 leuC, 1 hit |
PROSITEi | View protein in PROSITE PS00450 ACONITASE_1, 1 hit PS01244 ACONITASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WQF5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALQTGEPRT LAEKIWDDHI VVSGGGCAPD LIYIDLHLVH EVTSPQAFDG
60 70 80 90 100
LRLAGRRVRR PELTLATEDH NVPTVDIDQP IADPVSRTQV ETLRRNCAEF
110 120 130 140 150
GIRLHSMGDI EQGIVHVVGP QLGLTQPGMT IVCGDSHTST HGAFGALAMG
160 170 180 190 200
IGTSEVEHVL ATQTLPLRPF KTMAVNVDGR LPDGVSAKDI ILALIAKIGT
210 220 230 240 250
GGGQGHVIEY RGSAIESLSM EGRMTICNMS IEAGARAGMV APDETTYAFL
260 270 280 290 300
RGRPHAPTGA QWDTALVYWQ RLRTDVGAVF DTEVYLDAAS LSPFVTWGTN
310 320 330 340 350
PGQGVPLAAA VPDPQLMTDD AERQAAEKAL AYMDLRPGTA MRDIAVDAVF
360 370 380 390 400
VGSCTNGRIE DLRVVAEVLR GRKVADGVRM LIVPGSMRVR AQAEAEGLGE
410 420 430 440 450
IFTDAGAQWR QAGCSMCLGM NPDQLASGER CAATSNRNFE GRQGAGGRTH
460 470
LVSPAVAAAT AVRGTLSSPA DLN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45793.1 |
PIRi | G70853 |
RefSeqi | NP_217504.1, NC_000962.3 WP_003899570.1, NZ_NVQJ01000041.1 |
Genome annotation databases
EnsemblBacteriai | CCP45793; CCP45793; Rv2988c |
GeneIDi | 887875 |
KEGGi | mtu:Rv2988c mtv:RVBD_2988c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45793.1 |
PIRi | G70853 |
RefSeqi | NP_217504.1, NC_000962.3 WP_003899570.1, NZ_NVQJ01000041.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 83332.Rv2988c |
Proteomic databases
PaxDbi | P9WQF5 |
PRIDEi | P9WQF5 |
Genome annotation databases
EnsemblBacteriai | CCP45793; CCP45793; Rv2988c |
GeneIDi | 887875 |
KEGGi | mtu:Rv2988c mtv:RVBD_2988c |
Organism-specific databases
TubercuListi | Rv2988c |
Phylogenomic databases
eggNOGi | ENOG4105CQI Bacteria COG0065 LUCA |
KOi | K01703 |
OMAi | CNMSIEM |
PhylomeDBi | P9WQF5 |
Enzyme and pathway databases
UniPathwayi | UPA00048;UER00071 |
BioCyci | MTBH37RV:G185E-7243-MONOMER |
Family and domain databases
CDDi | cd01583 IPMI, 1 hit |
Gene3Di | 3.30.499.10, 1 hit |
HAMAPi | MF_01026 LeuC_type1, 1 hit |
InterProi | View protein in InterPro IPR004430 3-IsopropMal_deHydase_lsu IPR015931 Acnase/IPM_dHydase_lsu_aba_1/3 IPR001030 Acoase/IPM_deHydtase_lsu_aba IPR018136 Aconitase_4Fe-4S_BS IPR036008 Aconitase_4Fe-4S_dom IPR033941 IPMI_cat |
Pfami | View protein in Pfam PF00330 Aconitase, 1 hit |
PRINTSi | PR00415 ACONITASE |
SUPFAMi | SSF53732 SSF53732, 1 hit |
TIGRFAMsi | TIGR00170 leuC, 1 hit |
PROSITEi | View protein in PROSITE PS00450 ACONITASE_1, 1 hit PS01244 ACONITASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LEUC_MYCTU | |
Accessioni | P9WQF5Primary (citable) accession number: P9WQF5 Secondary accession number(s): L0TDZ8, O53237 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | October 16, 2019 | |
This is version 35 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways