UniProtKB - P9WQ85 (BIOF2_MYCTU)
Protein
Putative 8-amino-7-oxononanoate synthase 2
Gene
bioF2
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide.By similarity
Catalytic activityi
- EC:2.3.1.47
Cofactori
pyridoxal 5'-phosphateBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 407 | SubstrateBy similarity | 1 | |
Binding sitei | 510 | SubstrateBy similarity | 1 | |
Binding sitei | 556 | Pyridoxal phosphateBy similarity | 1 |
GO - Molecular functioni
- 8-amino-7-oxononanoate synthase activity Source: UniProtKB-EC
- pyridoxal phosphate binding Source: InterPro
GO - Biological processi
- biosynthetic process Source: InterPro
Keywordsi
Molecular function | Acyltransferase, Transferase |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-4146-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Putative 8-amino-7-oxononanoate synthase 2 (EC:2.3.1.47)Short name: AONS Alternative name(s): 7-keto-8-amino-pelargonic acid synthase Short name: 7-KAP synthase Short name: KAPA synthase 8-amino-7-ketopelargonate synthase |
Gene namesi | Name:bioF2 Ordered Locus Names:Rv0032 |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv0032 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000420959 | 1 – 771 | Putative 8-amino-7-oxononanoate synthase 2Add BLAST | 771 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 615 | N6-(pyridoxal phosphate)lysineBy similarity | 1 |
Proteomic databases
PaxDbi | P9WQ85 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 418 | UnknownAdd BLAST | 418 | |
Regioni | 419 – 771 | KAPA synthaseAdd BLAST | 353 | |
Regioni | 485 – 486 | Pyridoxal phosphate bindingBy similarity | 2 | |
Regioni | 581 – 584 | Pyridoxal phosphate bindingBy similarity | 4 |
Sequence similaritiesi
In the C-terminal section; belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily.Curated
Phylogenomic databases
eggNOGi | ENOG4107EEK Bacteria COG0156 LUCA |
KOi | K00652 |
OMAi | DALNHRG |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR016181 Acyl_CoA_acyltransferase IPR001917 Aminotrans_II_pyridoxalP_BS IPR004839 Aminotransferase_I/II IPR038740 BioF2-like_GNAT_dom IPR015424 PyrdxlP-dep_Trfase IPR015422 PyrdxlP-dep_Trfase_dom1 IPR015421 PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF13480 Acetyltransf_6, 1 hit PF00155 Aminotran_1_2, 1 hit |
SUPFAMi | SSF53383 SSF53383, 1 hit SSF55729 SSF55729, 1 hit |
PROSITEi | View protein in PROSITE PS00599 AA_TRANSFER_CLASS_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WQ85-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPTGLGYDFL RPVEDSGIND LKHYYFMADL ADGQPLGRAN LYSVCFDLAT
60 70 80 90 100
TDRKLTPAWR TTIKRWFPGF MTFRFLECGL LTMVSNPLAL RSDTDLERVL
110 120 130 140 150
PVLAGQMDQL AHDDGSDFLM IRDVDPEHYQ RYLDILRPLG FRPALGFSRV
160 170 180 190 200
DTTISWSSVE EALGCLSHKR RLPLKTSLEF RERFGIEVEE LDEYAEHAPV
210 220 230 240 250
LARLWRNVKT EAKDYQREDL NPEFFAACSR HLHGRSRLWL FRYQGTPIAF
260 270 280 290 300
FLNVWGADEN YILLEWGIDR DFEHYRKANL YRAALMLSLK DAISRDKRRM
310 320 330 340 350
EMGITNYFTK LRIPGARVIP TIYFLRHSTD PVHTATLARM MMHNIQRPTL
360 370 380 390 400
PDDMSEEFCR WEERIRLDQD GLPEHDIFRK IDRQHKYTGL KLGGVYGFYP
410 420 430 440 450
RFTGPQRSTV KAAELGEIVL LGTNSYLGLA THPEVVEASA EATRRYGTGC
460 470 480 490 500
SGSPLLNGTL DLHVSLEQEL ACFLGKPAAV LCSTGYQSNL AAISALCESG
510 520 530 540 550
DMIIQDALNH RSLFDAARLS GADFTLYRHN DMDHLARVLR RTEGRRRIIV
560 570 580 590 600
VDAVFSMEGT VADLATIAEL ADRHGCRVYV DESHALGVLG PDGRGASAAL
610 620 630 640 650
GVLARMDVVM GTFSKSFASV GGFIAGDRPV VDYIRHNGSG HVFSASLPPA
660 670 680 690 700
AAAATHAALR VSRREPDRRA RVLAAAEYMA TGLARQGYQA EYHGTAIVPV
710 720 730 740 750
ILGNPTVAHA GYLRLMRSGV YVNPVAPPAV PEERSGFRTS YLADHRQSDL
760 770
DRALHVFAGL AEDLTPQGAA L
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP42754.1 |
PIRi | F70701 |
RefSeqi | NP_214546.1, NC_000962.3 WP_003905217.1, NZ_NVQJ01000005.1 |
Genome annotation databases
EnsemblBacteriai | CCP42754; CCP42754; Rv0032 |
GeneIDi | 887050 |
KEGGi | mtu:Rv0032 mtv:RVBD_0032 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP42754.1 |
PIRi | F70701 |
RefSeqi | NP_214546.1, NC_000962.3 WP_003905217.1, NZ_NVQJ01000005.1 |
3D structure databases
SMRi | P9WQ85 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv0032 |
Proteomic databases
PaxDbi | P9WQ85 |
Genome annotation databases
EnsemblBacteriai | CCP42754; CCP42754; Rv0032 |
GeneIDi | 887050 |
KEGGi | mtu:Rv0032 mtv:RVBD_0032 |
Organism-specific databases
TubercuListi | Rv0032 |
Phylogenomic databases
eggNOGi | ENOG4107EEK Bacteria COG0156 LUCA |
KOi | K00652 |
OMAi | DALNHRG |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-4146-MONOMER |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR016181 Acyl_CoA_acyltransferase IPR001917 Aminotrans_II_pyridoxalP_BS IPR004839 Aminotransferase_I/II IPR038740 BioF2-like_GNAT_dom IPR015424 PyrdxlP-dep_Trfase IPR015422 PyrdxlP-dep_Trfase_dom1 IPR015421 PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF13480 Acetyltransf_6, 1 hit PF00155 Aminotran_1_2, 1 hit |
SUPFAMi | SSF53383 SSF53383, 1 hit SSF55729 SSF55729, 1 hit |
PROSITEi | View protein in PROSITE PS00599 AA_TRANSFER_CLASS_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BIOF2_MYCTU | |
Accessioni | P9WQ85Primary (citable) accession number: P9WQ85 Secondary accession number(s): L0T270, P71602, Q7DAK0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | September 18, 2019 | |
This is version 27 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names