UniProtKB - P9WPZ5 (DAPC_MYCTU)
Protein
Probable N-succinyldiaminopimelate aminotransferase DapC
Gene
dapC
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Involved in the lysine biosynthetic pathways. It catalyzes the transfer of an amino group from L-glutamate to N-succinyl-2-l-amino-6-oxoheptanedioate (N-succinyl-2-l-amino-6-ketopimelate) in a PLP-dependent reaction, yielding as products N-succinyl-l-2,6-diaminoheptanedioate (N-succinyl-diaminopimelate) and 2-oxoglutarate (Probable).1 Publication
Catalytic activityi
- EC:2.6.1.17
Cofactori
pyridoxal 5'-phosphateNote: Binds 1 pyridoxal phosphate per subunit.
: L-lysine biosynthesis via DAP pathway Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route).Proteins known to be involved in the 3 steps of the subpathway in this organism are:
- 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD)
- Probable N-succinyldiaminopimelate aminotransferase DapC (dapC)
- Putative succinyl-diaminopimelate desuccinylase DapE (dapE)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route), the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.
GO - Molecular functioni
- kynurenine-oxoglutarate transaminase activity Source: GO_Central
- pyridoxal phosphate binding Source: InterPro
- succinyldiaminopimelate transaminase activity Source: UniProtKB-EC
GO - Biological processi
- lysine biosynthetic process via diaminopimelate Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Aminotransferase, Transferase |
Biological process | Amino-acid biosynthesis, Lysine biosynthesis |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-5012-MONOMER |
UniPathwayi | UPA00034;UER00020 |
Names & Taxonomyi
Protein namesi | Recommended name: Probable N-succinyldiaminopimelate aminotransferase DapCAlternative name(s): DAP-AT (EC:2.6.1.17) |
Gene namesi | Name:dapC Ordered Locus Names:Rv0858c |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv0858c |
Subcellular locationi
- Cytoplasm Curated
GO - Cellular componenti
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000414591 | 1 – 397 | Probable N-succinyldiaminopimelate aminotransferase DapCAdd BLAST | 397 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 232 | N6-(pyridoxal phosphate)lysine | 1 |
Proteomic databases
PaxDbi | P9WPZ5 |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsProtein-protein interaction databases
STRINGi | 83332.Rv0858c |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P9WPZ5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 109 – 110 | Pyridoxal phosphate bindingBy similarity | 2 | |
Regioni | 218 – 222 | Pyridoxal phosphate bindingBy similarity | 5 |
Sequence similaritiesi
Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.Curated
Phylogenomic databases
eggNOGi | ENOG4105CHM Bacteria COG0436 LUCA |
KOi | K14267 |
OMAi | SVAMTGW |
PhylomeDBi | P9WPZ5 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839 Aminotransferase_I/II IPR015424 PyrdxlP-dep_Trfase IPR015422 PyrdxlP-dep_Trfase_dom1 IPR015421 PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF00155 Aminotran_1_2, 1 hit |
SUPFAMi | SSF53383 SSF53383, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WPZ5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTVSRLRPYA TTVFAEMSAL ATRIGAVNLG QGFPDEDGPP KMLQAAQDAI
60 70 80 90 100
AGGVNQYPPG PGSAPLRRAI AAQRRRHFGV DYDPETEVLV TVGATEAIAA
110 120 130 140 150
AVLGLVEPGS EVLLIEPFYD SYSPVVAMAG AHRVTVPLVP DGRGFALDAD
160 170 180 190 200
ALRRAVTPRT RALIINSPHN PTGAVLSATE LAAIAEIAVA ANLVVITDEV
210 220 230 240 250
YEHLVFDHAR HLPLAGFDGM AERTITISSA AKMFNCTGWK IGWACGPAEL
260 270 280 290 300
IAGVRAAKQY LSYVGGAPFQ PAVALALDTE DAWVAALRNS LRARRDRLAA
310 320 330 340 350
GLTEIGFAVH DSYGTYFLCA DPRPLGYDDS TEFCAALPEK VGVAAIPMSA
360 370 380 390
FCDPAAGQAS QQADVWNHLV RFTFCKRDDT LDEAIRRLSV LAERPAT
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP43606.1 |
PIRi | B70815 |
RefSeqi | NP_215373.1, NC_000962.3 WP_003404420.1, NZ_NVQJ01000040.1 |
Genome annotation databases
EnsemblBacteriai | CCP43606; CCP43606; Rv0858c |
GeneIDi | 885784 |
KEGGi | mtu:Rv0858c mtv:RVBD_0858c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP43606.1 |
PIRi | B70815 |
RefSeqi | NP_215373.1, NC_000962.3 WP_003404420.1, NZ_NVQJ01000040.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2O0R | X-ray | 2.00 | A/B | 2-397 | [»] | |
SMRi | P9WPZ5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv0858c |
Proteomic databases
PaxDbi | P9WPZ5 |
Genome annotation databases
EnsemblBacteriai | CCP43606; CCP43606; Rv0858c |
GeneIDi | 885784 |
KEGGi | mtu:Rv0858c mtv:RVBD_0858c |
Organism-specific databases
TubercuListi | Rv0858c |
Phylogenomic databases
eggNOGi | ENOG4105CHM Bacteria COG0436 LUCA |
KOi | K14267 |
OMAi | SVAMTGW |
PhylomeDBi | P9WPZ5 |
Enzyme and pathway databases
UniPathwayi | UPA00034;UER00020 |
BioCyci | MTBH37RV:G185E-5012-MONOMER |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839 Aminotransferase_I/II IPR015424 PyrdxlP-dep_Trfase IPR015422 PyrdxlP-dep_Trfase_dom1 IPR015421 PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF00155 Aminotran_1_2, 1 hit |
SUPFAMi | SSF53383 SSF53383, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DAPC_MYCTU | |
Accessioni | P9WPZ5Primary (citable) accession number: P9WPZ5 Secondary accession number(s): L0T542, O53870, Q7D954 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | October 16, 2019 | |
This is version 28 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references