UniProtKB - P9WPW7 (ASSY_MYCTU)
Protein
Argininosuccinate synthase
Gene
argG
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Catalytic activityi
- ATP + L-aspartate + L-citrulline = 2-(Nω-L-arginino)succinate + AMP + diphosphate + H+UniRule annotationEC:6.3.4.5UniRule annotation
: L-arginine biosynthesis Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotationProteins known to be involved in the 3 steps of the subpathway in this organism are:
- Ornithine carbamoyltransferase (argF)
- Argininosuccinate synthase (argG)
- Argininosuccinate lyase (argH)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 87 | CitrullineUniRule annotation | 1 | |
Binding sitei | 117 | ATP; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 119 | AspartateUniRule annotation | 1 | |
Binding sitei | 123 | AspartateUniRule annotation | 1 | |
Binding sitei | 123 | CitrullineUniRule annotation | 1 | |
Binding sitei | 124 | AspartateUniRule annotation | 1 | |
Binding sitei | 127 | CitrullineUniRule annotation | 1 | |
Binding sitei | 175 | CitrullineUniRule annotation | 1 | |
Binding sitei | 260 | CitrullineUniRule annotation | 1 | |
Binding sitei | 272 | CitrullineUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 8 – 16 | ATPUniRule annotation | 9 |
GO - Molecular functioni
- argininosuccinate synthase activity Source: GO_Central
- ATP binding Source: UniProtKB-UniRule
GO - Biological processi
- arginine biosynthetic process Source: GO_Central
- argininosuccinate metabolic process Source: GO_Central
- urea cycle Source: GO_Central
Keywordsi
Molecular function | Ligase |
Biological process | Amino-acid biosynthesis, Arginine biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-5848-MONOMER |
UniPathwayi | UPA00068;UER00113 |
Names & Taxonomyi
Protein namesi | Recommended name: Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)Alternative name(s): Citrulline--aspartate ligaseUniRule annotation |
Gene namesi | Name:argGUniRule annotation Ordered Locus Names:Rv1658 ORF Names:MTCY06H11.23 |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv1658 |
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: GO_Central
- cytosol Source: MTBBASE
- plasma membrane Source: MTBBASE
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000148616 | 1 – 398 | Argininosuccinate synthaseAdd BLAST | 398 |
Proteomic databases
PaxDbi | P9WPW7 |
Interactioni
Subunit structurei
Homotetramer.
UniRule annotationProtein-protein interaction databases
STRINGi | 83332.Rv1658 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG4105CDH Bacteria COG0137 LUCA |
KOi | K01940 |
OMAi | QCEVVTF |
PhylomeDBi | P9WPW7 |
Family and domain databases
CDDi | cd01999 Argininosuccinate_Synthase, 1 hit |
Gene3Di | 3.40.50.620, 1 hit 3.90.1260.10, 1 hit |
HAMAPi | MF_00005 Arg_succ_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR001518 Arginosuc_synth IPR018223 Arginosuc_synth_CS IPR023434 Arginosuc_synth_type_1_subfam IPR024074 AS_cat/multimer_dom_body IPR014729 Rossmann-like_a/b/a_fold |
PANTHERi | PTHR11587 PTHR11587, 1 hit |
Pfami | View protein in Pfam PF00764 Arginosuc_synth, 1 hit |
SUPFAMi | SSF69864 SSF69864, 1 hit |
TIGRFAMsi | TIGR00032 argG, 1 hit |
PROSITEi | View protein in PROSITE PS00564 ARGININOSUCCIN_SYN_1, 1 hit PS00565 ARGININOSUCCIN_SYN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WPW7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSERVILAYS GGLDTSVAIS WIGKETGREV VAVAIDLGQG GEHMDVIRQR
60 70 80 90 100
ALDCGAVEAV VVDARDEFAE GYCLPTVLNN ALYMDRYPLV SAISRPLIVK
110 120 130 140 150
HLVAAAREHG GGIVAHGCTG KGNDQVRFEV GFASLAPDLE VLAPVRDYAW
160 170 180 190 200
TREKAIAFAE ENAIPINVTK RSPFSIDQNV WGRAVETGFL EHLWNAPTKD
210 220 230 240 250
IYAYTEDPTI NWGVPDEVIV GFERGVPVSV DGKPVSMLAA IEELNRRAGA
260 270 280 290 300
QGVGRLDVVE DRLVGIKSRE IYEAPGAMVL ITAHTELEHV TLERELGRFK
310 320 330 340 350
RQTDQRWAEL VYDGLWYSPL KAALEAFVAK TQEHVSGEVR LVLHGGHIAV
360 370 380 390
NGRRSAESLY DFNLATYDEG DSFDQSAARG FVYVHGLSSK LAARRDLR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP44423.1 |
PIRi | E70621 |
RefSeqi | NP_216174.1, NC_000962.3 WP_003408179.1, NZ_NVQJ01000069.1 |
Genome annotation databases
EnsemblBacteriai | CCP44423; CCP44423; Rv1658 |
GeneIDi | 885645 |
KEGGi | mtu:Rv1658 mtv:RVBD_1658 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP44423.1 |
PIRi | E70621 |
RefSeqi | NP_216174.1, NC_000962.3 WP_003408179.1, NZ_NVQJ01000069.1 |
3D structure databases
SMRi | P9WPW7 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv1658 |
Proteomic databases
PaxDbi | P9WPW7 |
Genome annotation databases
EnsemblBacteriai | CCP44423; CCP44423; Rv1658 |
GeneIDi | 885645 |
KEGGi | mtu:Rv1658 mtv:RVBD_1658 |
Organism-specific databases
TubercuListi | Rv1658 |
Phylogenomic databases
eggNOGi | ENOG4105CDH Bacteria COG0137 LUCA |
KOi | K01940 |
OMAi | QCEVVTF |
PhylomeDBi | P9WPW7 |
Enzyme and pathway databases
UniPathwayi | UPA00068;UER00113 |
BioCyci | MTBH37RV:G185E-5848-MONOMER |
Family and domain databases
CDDi | cd01999 Argininosuccinate_Synthase, 1 hit |
Gene3Di | 3.40.50.620, 1 hit 3.90.1260.10, 1 hit |
HAMAPi | MF_00005 Arg_succ_synth_type1, 1 hit |
InterProi | View protein in InterPro IPR001518 Arginosuc_synth IPR018223 Arginosuc_synth_CS IPR023434 Arginosuc_synth_type_1_subfam IPR024074 AS_cat/multimer_dom_body IPR014729 Rossmann-like_a/b/a_fold |
PANTHERi | PTHR11587 PTHR11587, 1 hit |
Pfami | View protein in Pfam PF00764 Arginosuc_synth, 1 hit |
SUPFAMi | SSF69864 SSF69864, 1 hit |
TIGRFAMsi | TIGR00032 argG, 1 hit |
PROSITEi | View protein in PROSITE PS00564 ARGININOSUCCIN_SYN_1, 1 hit PS00565 ARGININOSUCCIN_SYN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ASSY_MYCTU | |
Accessioni | P9WPW7Primary (citable) accession number: P9WPW7 Secondary accession number(s): L0TA96, P63642, P94993 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | November 13, 2019 | |
This is version 30 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways