UniProtKB - P9WPA9 (KCY_MYCTU)
Protein
Cytidylate kinase
Gene
cmk
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Catalytic activityi
Kineticsi
- KM=120 µM for CMP1 Publication
- KM=165 µM for dCMP1 Publication
- KM=13.9 mM for UMP1 Publication
- Vmax=131 µmol/min/mg enzyme with CMP as substrate1 Publication
- Vmax=75 µmol/min/mg enzyme with dCMP as substrate1 Publication
- Vmax=32 µmol/min/mg enzyme with UMP as substrate1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 13 – 21 | ATPUniRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- cytidylate kinase activity Source: MTBBASE
GO - Biological processi
- nucleobase-containing small molecule interconversion Source: GO_Central
- pyrimidine nucleotide metabolic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Cytidylate kinaseUniRule annotation (EC:2.7.4.25UniRule annotation)Short name: CKUniRule annotation Alternative name(s): Cytidine monophosphate kinaseUniRule annotation Short name: CMP kinaseUniRule annotation |
Gene namesi | Name:cmkUniRule annotation Ordered Locus Names:Rv1712 ORF Names:MTCI125.34 |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv1712 |
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytosol Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000131943 | 2 – 230 | Cytidylate kinaseAdd BLAST | 229 |
Proteomic databases
PaxDbi | P9WPA9 |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
STRINGi | 83332.Rv1712 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0283, Bacteria |
OMAi | RAITWWM |
PhylomeDBi | P9WPA9 |
Family and domain databases
CDDi | cd02020, CMPK, 1 hit |
HAMAPi | MF_00238, Cytidyl_kinase_type1, 1 hit |
InterProi | View protein in InterPro IPR003136, Cytidylate_kin IPR011994, Cytidylate_kinase_dom IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF02224, Cytidylate_kin, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00017, cmk, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P9WPA9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSRLSAAVVA IDGPAGTGKS SVSRRLAREL GARFLDTGAM YRIVTLAVLR
60 70 80 90 100
AGADPSDIAA VETIASTVQM SLGYDPDGDS CYLAGEDVSV EIRGDAVTRA
110 120 130 140 150
VSAVSSVPAV RTRLVELQRT MAEGPGSIVV EGRDIGTVVF PDAPVKIFLT
160 170 180 190 200
ASAETRARRR NAQNVAAGLA DDYDGVLADV RRRDHLDSTR AVSPLQAAGD
210 220 230
AVIVDTSDMT EAEVVAHLLE LVTRRSEAVR
Mass spectrometryi
Molecular mass is 23930.56 Da. Determined by ESI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP44478.1 |
PIRi | G70504 |
RefSeqi | NP_216228.1, NC_000962.3 WP_003408441.1, NZ_NVQJ01000010.1 |
Genome annotation databases
EnsemblBacteriai | CCP44478; CCP44478; Rv1712 |
GeneIDi | 23492120 885157 |
KEGGi | mtu:Rv1712 mtv:RVBD_1712 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP44478.1 |
PIRi | G70504 |
RefSeqi | NP_216228.1, NC_000962.3 WP_003408441.1, NZ_NVQJ01000010.1 |
3D structure databases
SMRi | P9WPA9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv1712 |
Proteomic databases
PaxDbi | P9WPA9 |
Genome annotation databases
EnsemblBacteriai | CCP44478; CCP44478; Rv1712 |
GeneIDi | 23492120 885157 |
KEGGi | mtu:Rv1712 mtv:RVBD_1712 |
Organism-specific databases
TubercuListi | Rv1712 |
Phylogenomic databases
eggNOGi | COG0283, Bacteria |
OMAi | RAITWWM |
PhylomeDBi | P9WPA9 |
Family and domain databases
CDDi | cd02020, CMPK, 1 hit |
HAMAPi | MF_00238, Cytidyl_kinase_type1, 1 hit |
InterProi | View protein in InterPro IPR003136, Cytidylate_kin IPR011994, Cytidylate_kinase_dom IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF02224, Cytidylate_kin, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00017, cmk, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KCY_MYCTU | |
Accessioni | P9WPA9Primary (citable) accession number: P9WPA9 Secondary accession number(s): L0TAE5, O33211, P63803 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | December 2, 2020 | |
This is version 37 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families