UniProtKB - P9WP97 (COBB_MYCTU)
Protein
Hydrogenobyrinate a,c-diamide synthase
Gene
cobB
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Catalyzes the ATP-dependent amidation of the two carboxylate groups at positions a and c of hydrogenobyrinate, using either L-glutamine or ammonia as the nitrogen source.UniRule annotation
Miscellaneous
The a and c carboxylates of hydrogenobyrinate are activated for nucleophilic attack via formation of a phosphorylated intermediate by ATP. CobB catalyzes first the amidation of the c-carboxylate, and then that of the a-carboxylate.UniRule annotation
Catalytic activityi
- 2 ATP + 2 H2O + hydrogenobyrinate + 2 L-glutamine = 2 ADP + 2 H+ + hydrogenobyrinate a,c-diamide + 2 L-glutamate + 2 phosphateUniRule annotationEC:6.3.5.9UniRule annotation
Cofactori
Mg2+UniRule annotation
: adenosylcobalamin biosynthesis Pathwayi
This protein is involved in step 9 of the subpathway that synthesizes cob(II)yrinate a,c-diamide from precorrin-2 (aerobic route).UniRule annotationProteins known to be involved in the 10 steps of the subpathway in this organism are:
- Cobalamin biosynthesis protein CobIJ (cobIJ)
- no protein annotated in this organism
- Cobalamin biosynthesis protein CobIJ (cobIJ)
- Precorrin-4 C(11)-methyltransferase (cobM)
- no protein annotated in this organism
- Precorrin-6A reductase (cobK)
- Precorrin-6Y C(5,15)-methyltransferase [decarboxylating] (cobL)
- Precorrin-8X methylmutase (cobH)
- Hydrogenobyrinate a,c-diamide synthase (cobB)
- no protein annotated in this organism
View all proteins of this organism that are known to be involved in the subpathway that synthesizes cob(II)yrinate a,c-diamide from precorrin-2 (aerobic route), the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 337 | NucleophileUniRule annotation | 1 | |
Sitei | 433 | Increases nucleophilicity of active site CysUniRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- cobyrinic acid a,c-diamide synthase activity Source: InterPro
- hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) activity Source: UniProtKB-UniRule
GO - Biological processi
- cobalamin biosynthetic process Source: UniProtKB-UniRule
- glutamine metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Ligase |
Biological process | Cobalamin biosynthesis |
Ligand | ATP-binding, Magnesium, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-7099-MONOMER |
UniPathwayi | UPA00148;UER00220 |
Names & Taxonomyi
Protein namesi | Recommended name: Hydrogenobyrinate a,c-diamide synthaseUniRule annotation (EC:6.3.5.9UniRule annotation)Alternative name(s): Hydrogenobyrinic acid a,c-diamide synthaseUniRule annotation |
Gene namesi | Name:cobBUniRule annotation Ordered Locus Names:Rv2848c ORF Names:MTCY24A1.09 |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv2848c |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000141262 | 1 – 457 | Hydrogenobyrinate a,c-diamide synthaseAdd BLAST | 457 |
Proteomic databases
PaxDbi | P9WP97 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 255 – 441 | GATase cobBQ-typeUniRule annotationAdd BLAST | 187 |
Domaini
Comprises of two domains. The C-terminal domain contains the binding site for glutamine and catalyzes the hydrolysis of this substrate to glutamate and ammonia. The N-terminal domain is anticipated to bind ATP and hydrogenobyrinate and catalyzes the ultimate synthesis of the diamide product. The ammonia produced via the glutaminase domain is probably translocated to the adjacent domain via a molecular tunnel, where it reacts with an activated intermediate.UniRule annotation
Sequence similaritiesi
Belongs to the CobB/CbiA family.UniRule annotation
Keywords - Domaini
Glutamine amidotransferasePhylogenomic databases
eggNOGi | ENOG4105C0Y Bacteria COG1797 LUCA |
KOi | K02224 |
OMAi | QPFKCGP |
PhylomeDBi | P9WP97 |
Family and domain databases
Gene3Di | 3.40.50.880, 1 hit |
HAMAPi | MF_00027 CobB_CbiA, 1 hit |
InterProi | View protein in InterPro IPR004484 CbiA_synth IPR029062 Class_I_gatase-like IPR017929 CobB/CobQ_GATase IPR002586 CobQ/CobB/MinD/ParA_Nub-bd_dom IPR011698 GATase_3 IPR027417 P-loop_NTPase |
PANTHERi | PTHR43873 PTHR43873, 1 hit |
Pfami | View protein in Pfam PF01656 CbiA, 1 hit PF07685 GATase_3, 1 hit |
SUPFAMi | SSF52317 SSF52317, 1 hit SSF52540 SSF52540, 1 hit |
TIGRFAMsi | TIGR00379 cobB, 1 hit |
PROSITEi | View protein in PROSITE PS51274 GATASE_COBBQ, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WP97-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRVSAVAVAA PASGSGKTTI ATGLIGALRQ AGHTVAPFKV GPDFIDPGYH
60 70 80 90 100
ALAAGRPGRN LDPVLVGERL IGPLYAHGVA GADIAVIEGV LGLFDGRIGP
110 120 130 140 150
AGGAPAAGST AHVAALLGAP VILVVDARGQ SHSVAALLHG FSTFDTATRI
160 170 180 190 200
AGVILNRVGS ARHEQVLRQA CDQAGVAVLG AIPRTAELEL PTRYLGLVTA
210 220 230 240 250
VEYGRRARLA VQAMTAVVAR HVDLAAVIAC AGSQAAHPPW DPVIAVGNTA
260 270 280 290 300
RQPATVAIAA GRAFTFGYAE HAEMLRAAGA EVVEFDPLSE TLPEGTDAVV
310 320 330 340 350
LPGGFPEQFT AELSANDTVR RQINELAAAG APVHAECAGL LYLVSELDGH
360 370 380 390 400
PMCGVVAGSA RFTQHLKLGY RDAVAVVDSA LYSVGERVVG HEFHRTAVTF
410 420 430 440 450
ADSYQPAWVY QGQDVDDVRD GAVHSGVHAS YLHTHPAATP GAVARFVAHA
ACNTPRA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45649.1 |
PIRi | C70589 |
RefSeqi | NP_217364.1, NC_000962.3 WP_003899509.1, NZ_NVQJ01000006.1 |
Genome annotation databases
EnsemblBacteriai | CCP45649; CCP45649; Rv2848c |
GeneIDi | 888499 |
KEGGi | mtu:Rv2848c mtv:RVBD_2848c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45649.1 |
PIRi | C70589 |
RefSeqi | NP_217364.1, NC_000962.3 WP_003899509.1, NZ_NVQJ01000006.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 83332.Rv2848c |
Proteomic databases
PaxDbi | P9WP97 |
Genome annotation databases
EnsemblBacteriai | CCP45649; CCP45649; Rv2848c |
GeneIDi | 888499 |
KEGGi | mtu:Rv2848c mtv:RVBD_2848c |
Organism-specific databases
TubercuListi | Rv2848c |
Phylogenomic databases
eggNOGi | ENOG4105C0Y Bacteria COG1797 LUCA |
KOi | K02224 |
OMAi | QPFKCGP |
PhylomeDBi | P9WP97 |
Enzyme and pathway databases
UniPathwayi | UPA00148;UER00220 |
BioCyci | MTBH37RV:G185E-7099-MONOMER |
Family and domain databases
Gene3Di | 3.40.50.880, 1 hit |
HAMAPi | MF_00027 CobB_CbiA, 1 hit |
InterProi | View protein in InterPro IPR004484 CbiA_synth IPR029062 Class_I_gatase-like IPR017929 CobB/CobQ_GATase IPR002586 CobQ/CobB/MinD/ParA_Nub-bd_dom IPR011698 GATase_3 IPR027417 P-loop_NTPase |
PANTHERi | PTHR43873 PTHR43873, 1 hit |
Pfami | View protein in Pfam PF01656 CbiA, 1 hit PF07685 GATase_3, 1 hit |
SUPFAMi | SSF52317 SSF52317, 1 hit SSF52540 SSF52540, 1 hit |
TIGRFAMsi | TIGR00379 cobB, 1 hit |
PROSITEi | View protein in PROSITE PS51274 GATASE_COBBQ, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | COBB_MYCTU | |
Accessioni | P9WP97Primary (citable) accession number: P9WP97 Secondary accession number(s): L0TCF1, O05811, P63835 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | September 18, 2019 | |
This is version 28 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways