UniProtKB - P9WNC3 (FPG1_MYCTU)
Protein
Formamidopyrimidine-DNA glycosylase 1
Gene
fpg1
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG) when paired with C, G or T, as well as methyl-faPy (formanidopyrimidine residues) in poly(dG-dC) and spiroiminodihydantoin:C base pairs. Unlike its E.coli ortholog has no activity on 8-oxoG:A. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Cleaves ssDNA containing an AP site. Complements the H2O2 sensitivity of an M.smegmatis fpg disruption mutant; upon expression in M.smegmatis excises 8-oxoG from dsDNA.3 Publications
Catalytic activityi
- Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine. EC:3.2.2.23
- The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. EC:4.2.99.18
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 2 | Schiff-base intermediate with DNABy similarity | 1 | |
Active sitei | 3 | Proton donorBy similarity | 1 | |
Active sitei | 61 | Proton donor; for beta-elimination activityBy similarity | 1 | |
Binding sitei | 100 | DNABy similarity | 1 | |
Binding sitei | 119 | DNABy similarity | 1 | |
Binding sitei | 165 | DNABy similarity | 1 | |
Active sitei | 275 | Proton donor; for delta-elimination activityBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 251 – 285 | FPG-typeAdd BLAST | 35 |
GO - Molecular functioni
- 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity Source: MTBBASE
- class I DNA-(apurinic or apyrimidinic site) endonuclease activity Source: UniProtKB-EC
- damaged DNA binding Source: InterPro
- DNA-(apurinic or apyrimidinic site) endonuclease activity Source: GO_Central
- DNA N-glycosylase activity Source: GO_Central
- double-stranded DNA binding Source: MTBBASE
- oxidized purine nucleobase lesion DNA N-glycosylase activity Source: MTBBASE
- zinc ion binding Source: UniProtKB-UniRule
GO - Biological processi
- base-excision repair Source: MTBBASE
- base-excision repair, AP site formation Source: GO_Central
- DNA repair Source: MTBBASE
- nucleotide-excision repair Source: InterPro
- response to oxidative stress Source: MTBBASE
Keywordsi
Molecular function | DNA-binding, Glycosidase, Hydrolase, Lyase, Multifunctional enzyme |
Biological process | DNA damage, DNA repair |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-7177-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:fpg1 Synonyms:mutM Ordered Locus Names:Rv2924c ORF Names:MTCY338.13c |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv2924c |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000170840 | 2 – 289 | Formamidopyrimidine-DNA glycosylase 1Add BLAST | 288 |
Proteomic databases
PaxDbi | P9WNC3 |
PRIDEi | P9WNC3 |
Expressioni
Inductioni
Expressed in mid-log phase.1 Publication
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
STRINGi | 83332.Rv2924c |
Family & Domainsi
Sequence similaritiesi
Belongs to the FPG family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 251 – 285 | FPG-typeAdd BLAST | 35 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | ENOG4105ERD Bacteria COG0266 LUCA |
KOi | K10563 |
OMAi | GVHLRMT |
PhylomeDBi | P9WNC3 |
Family and domain databases
Gene3Di | 3.20.190.10, 1 hit |
HAMAPi | MF_00103 Fapy_DNA_glycosyl, 1 hit |
InterProi | View protein in InterPro IPR015886 DNA_glyclase/AP_lyase_DNA-bd IPR015887 DNA_glyclase_Znf_dom_DNA_BS IPR020629 Formamido-pyr_DNA_Glyclase IPR012319 FPG_cat IPR035937 MutM-like_N-ter IPR010979 Ribosomal_S13-like_H2TH IPR000214 Znf_DNA_glyclase/AP_lyase IPR010663 Znf_FPG/IleRS |
Pfami | View protein in Pfam PF01149 Fapy_DNA_glyco, 1 hit PF06831 H2TH, 1 hit PF06827 zf-FPG_IleRS, 1 hit |
SMARTi | View protein in SMART SM00898 Fapy_DNA_glyco, 1 hit SM01232 H2TH, 1 hit |
SUPFAMi | SSF46946 SSF46946, 1 hit SSF81624 SSF81624, 1 hit |
TIGRFAMsi | TIGR00577 fpg, 1 hit |
PROSITEi | View protein in PROSITE PS51068 FPG_CAT, 1 hit PS01242 ZF_FPG_1, 1 hit PS51066 ZF_FPG_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P9WNC3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPELPEVEVV RRGLQAHVTG RTITEVRVHH PRAVRRHDAG PADLTARLRG
60 70 80 90 100
ARINGTDRRG KYLWLTLNTA GVHRPTDTAL VVHLGMSGQM LLGAVPCAAH
110 120 130 140 150
VRISALLDDG TVLSFADQRT FGGWLLADLV TVDGSVVPVP VAHLARDPLD
160 170 180 190 200
PRFDCDAVVK VLRRKHSELK RQLLDQRVVS GIGNIYADEA LWRAKVNGAH
210 220 230 240 250
VAATLRCRRL GAVLHAAADV MREALAKGGT SFDSLYVNVN GESGYFERSL
260 270 280
DAYGREGENC RRCGAVIRRE RFMNRSSFYC PRCQPRPRK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45727.1 |
PIRi | D70748 |
RefSeqi | NP_217440.1, NC_000962.3 WP_003414814.1, NZ_NVQJ01000006.1 |
Genome annotation databases
EnsemblBacteriai | CCP45727; CCP45727; Rv2924c |
GeneIDi | 887438 |
KEGGi | mtu:Rv2924c mtv:RVBD_2924c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45727.1 |
PIRi | D70748 |
RefSeqi | NP_217440.1, NC_000962.3 WP_003414814.1, NZ_NVQJ01000006.1 |
3D structure databases
SMRi | P9WNC3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv2924c |
Proteomic databases
PaxDbi | P9WNC3 |
PRIDEi | P9WNC3 |
Genome annotation databases
EnsemblBacteriai | CCP45727; CCP45727; Rv2924c |
GeneIDi | 887438 |
KEGGi | mtu:Rv2924c mtv:RVBD_2924c |
Organism-specific databases
TubercuListi | Rv2924c |
Phylogenomic databases
eggNOGi | ENOG4105ERD Bacteria COG0266 LUCA |
KOi | K10563 |
OMAi | GVHLRMT |
PhylomeDBi | P9WNC3 |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-7177-MONOMER |
Family and domain databases
Gene3Di | 3.20.190.10, 1 hit |
HAMAPi | MF_00103 Fapy_DNA_glycosyl, 1 hit |
InterProi | View protein in InterPro IPR015886 DNA_glyclase/AP_lyase_DNA-bd IPR015887 DNA_glyclase_Znf_dom_DNA_BS IPR020629 Formamido-pyr_DNA_Glyclase IPR012319 FPG_cat IPR035937 MutM-like_N-ter IPR010979 Ribosomal_S13-like_H2TH IPR000214 Znf_DNA_glyclase/AP_lyase IPR010663 Znf_FPG/IleRS |
Pfami | View protein in Pfam PF01149 Fapy_DNA_glyco, 1 hit PF06831 H2TH, 1 hit PF06827 zf-FPG_IleRS, 1 hit |
SMARTi | View protein in SMART SM00898 Fapy_DNA_glyco, 1 hit SM01232 H2TH, 1 hit |
SUPFAMi | SSF46946 SSF46946, 1 hit SSF81624 SSF81624, 1 hit |
TIGRFAMsi | TIGR00577 fpg, 1 hit |
PROSITEi | View protein in PROSITE PS51068 FPG_CAT, 1 hit PS01242 ZF_FPG_1, 1 hit PS51066 ZF_FPG_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FPG1_MYCTU | |
Accessioni | P9WNC3Primary (citable) accession number: P9WNC3 Secondary accession number(s): L0TDY9, P64150, Q10959 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | September 18, 2019 | |
This is version 31 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names