UniProtKB - P9WMN1 (HIS1_MYCTU)
Protein
ATP phosphoribosyltransferase
Gene
hisG
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity (By similarity).By similarity
Catalytic activityi
- EC:2.4.2.17
Cofactori
Activity regulationi
Feedback inhibited by histidine. Also inhibited by AMP.
: L-histidine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.Proteins known to be involved in the 9 steps of the subpathway in this organism are:
- ATP phosphoribosyltransferase (hisG)
- Phosphoribosyl-ATP pyrophosphatase (hisE)
- Phosphoribosyl-AMP cyclohydrolase (hisI)
- Phosphoribosyl isomerase A (priA)
- Imidazole glycerol phosphate synthase subunit HisH (hisH), Imidazole glycerol phosphate synthase subunit HisF (hisF)
- Imidazoleglycerol-phosphate dehydratase (hisB)
- Histidinol-phosphate aminotransferase (hisC)
- Histidinol-phosphatase (hisN)
- Histidinol dehydrogenase (hisD)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.
GO - Molecular functioni
- AMP binding Source: MTBBASE
- ATP binding Source: MTBBASE
- ATP phosphoribosyltransferase activity Source: MTBBASE
- magnesium ion binding Source: MTBBASE
GO - Biological processi
- histidine biosynthetic process Source: MTBBASE
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Amino-acid biosynthesis, Histidine biosynthesis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
UniPathwayi | UPA00031;UER00006 |
Names & Taxonomyi
Protein namesi | Recommended name: ATP phosphoribosyltransferase (EC:2.4.2.17)Short name: ATP-PRT Short name: ATP-PRTase |
Gene namesi | Name:hisG Ordered Locus Names:Rv2121c ORF Names:MTCY261.17c |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv2121c |
Subcellular locationi
- Cytoplasm By similarity
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
- plasma membrane Source: MTBBASE
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000151857 | 1 – 284 | ATP phosphoribosyltransferaseAdd BLAST | 284 |
Proteomic databases
PaxDbi | P9WMN1 |
Interactioni
Subunit structurei
Equilibrium between an active dimeric form, an inactive hexameric form and higher aggregates. Interconversion between the various forms is largely reversible and is influenced by the natural substrates and inhibitors of the enzyme.
1 PublicationProtein-protein interaction databases
STRINGi | 83332.Rv2121c |
Chemistry databases
BindingDBi | P9WMN1 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P9WMN1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0040, Bacteria |
OMAi | YVMMDYD |
PhylomeDBi | P9WMN1 |
Family and domain databases
Gene3Di | 3.30.70.120, 1 hit |
HAMAPi | MF_00079, HisG_Long, 1 hit |
InterProi | View protein in InterPro IPR013820, ATP_PRibTrfase_cat IPR018198, ATP_PRibTrfase_CS IPR001348, ATP_PRibTrfase_HisG IPR020621, ATP_PRibTrfase_HisG_long IPR013115, HisG_C IPR011322, N-reg_PII-like_a/b IPR015867, N-reg_PII/ATP_PRibTrfase_C |
PANTHERi | PTHR21403, PTHR21403, 1 hit |
Pfami | View protein in Pfam PF01634, HisG, 1 hit PF08029, HisG_C, 1 hit |
SUPFAMi | SSF54913, SSF54913, 1 hit |
TIGRFAMsi | TIGR00070, hisG, 1 hit TIGR03455, HisG_C-term, 1 hit |
PROSITEi | View protein in PROSITE PS01316, ATP_P_PHORIBOSYLTR, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WMN1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLRVAVPNKG ALSEPATEIL AEAGYRRRTD SKDLTVIDPV NNVEFFFLRP
60 70 80 90 100
KDIAIYVGSG ELDFGITGRD LVCDSGAQVR ERLALGFGSS SFRYAAPAGR
110 120 130 140 150
NWTTADLAGM RIATAYPNLV RKDLATKGIE ATVIRLDGAV EISVQLGVAD
160 170 180 190 200
AIADVVGSGR TLSQHDLVAF GEPLCDSEAV LIERAGTDGQ DQTEARDQLV
210 220 230 240 250
ARVQGVVFGQ QYLMLDYDCP RSALKKATAI TPGLESPTIA PLADPDWVAI
260 270 280
RALVPRRDVN GIMDELAAIG AKAILASDIR FCRF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP44896.1 |
PIRi | D70513 |
RefSeqi | NP_216637.1, NC_000962.3 WP_003411047.1, NZ_NVQJ01000058.1 |
Genome annotation databases
EnsemblBacteriai | CCP44896; CCP44896; Rv2121c |
GeneIDi | 23491691 888689 |
KEGGi | mtu:Rv2121c mtv:RVBD_2121c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP44896.1 |
PIRi | D70513 |
RefSeqi | NP_216637.1, NC_000962.3 WP_003411047.1, NZ_NVQJ01000058.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1NH7 | X-ray | 2.70 | A | 1-284 | [»] | |
1NH8 | X-ray | 1.80 | A | 1-284 | [»] | |
5LHT | X-ray | 2.06 | A | 1-284 | [»] | |
5LHU | X-ray | 2.02 | A | 1-284 | [»] | |
5U99 | X-ray | 2.40 | A | 1-284 | [»] | |
SMRi | P9WMN1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv2121c |
Chemistry databases
BindingDBi | P9WMN1 |
ChEMBLi | CHEMBL6086 |
DrugCentrali | P9WMN1 |
Proteomic databases
PaxDbi | P9WMN1 |
Genome annotation databases
EnsemblBacteriai | CCP44896; CCP44896; Rv2121c |
GeneIDi | 23491691 888689 |
KEGGi | mtu:Rv2121c mtv:RVBD_2121c |
Organism-specific databases
TubercuListi | Rv2121c |
Phylogenomic databases
eggNOGi | COG0040, Bacteria |
OMAi | YVMMDYD |
PhylomeDBi | P9WMN1 |
Enzyme and pathway databases
UniPathwayi | UPA00031;UER00006 |
Family and domain databases
Gene3Di | 3.30.70.120, 1 hit |
HAMAPi | MF_00079, HisG_Long, 1 hit |
InterProi | View protein in InterPro IPR013820, ATP_PRibTrfase_cat IPR018198, ATP_PRibTrfase_CS IPR001348, ATP_PRibTrfase_HisG IPR020621, ATP_PRibTrfase_HisG_long IPR013115, HisG_C IPR011322, N-reg_PII-like_a/b IPR015867, N-reg_PII/ATP_PRibTrfase_C |
PANTHERi | PTHR21403, PTHR21403, 1 hit |
Pfami | View protein in Pfam PF01634, HisG, 1 hit PF08029, HisG_C, 1 hit |
SUPFAMi | SSF54913, SSF54913, 1 hit |
TIGRFAMsi | TIGR00070, hisG, 1 hit TIGR03455, HisG_C-term, 1 hit |
PROSITEi | View protein in PROSITE PS01316, ATP_P_PHORIBOSYLTR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HIS1_MYCTU | |
Accessioni | P9WMN1Primary (citable) accession number: P9WMN1 Secondary accession number(s): L0TBL0, O33256, P60759 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | December 2, 2020 | |
This is version 33 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families