UniProtKB - P9WMC3 (HTHR_MYCTU)
Protein
HTH-type transcriptional repressor Rv3405c
Gene
Rv3405c
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Negatively regulates the expression of sulfate ester dioxygenase Rv3406 and its own expression (PubMed:25427196, PubMed:29395080). Binds specifically to the inverted repeat sequence of the intergenic region between Rv3405c and Rv3406 (PubMed:29395080).2 Publications
Miscellaneous
Was identified as a high-confidence drug target.1 Publication
Activity regulationi
Specifically binds to tetracycline, which leads to a conformational change in the structure of the protein and inhibits the DNA binding activity.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 40 – 59 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- DNA-binding transcription factor activity Source: GO_Central
- transcription regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- regulation of transcription, DNA-templated Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: HTH-type transcriptional repressor Rv3405cCurated |
Gene namesi | Ordered Locus Names:Rv3405c ORF Names:MTCY78.23 |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv3405c |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 52 | H → A: Abolishes DNA-binding and repressor activity; when associated with A-57 and A-62. 1 Publication | 1 | |
Mutagenesisi | 57 | R → A: Abolishes DNA-binding and repressor activity; when associated with A-52 and A-62. 1 Publication | 1 | |
Mutagenesisi | 62 | K → A: Abolishes DNA-binding and repressor activity; when associated with A-52 and A-57. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000070672 | 1 – 188 | HTH-type transcriptional repressor Rv3405cAdd BLAST | 188 |
Proteomic databases
PaxDbi | P9WMC3 |
Expressioni
Inductioni
Negatively autoregulated.2 Publications
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 17 – 77 | HTH tetR-typePROSITE-ProRule annotationAdd BLAST | 61 |
Domaini
Contains an N-terminal DNA-binding domain and a C-terminal ligand binding pocket.1 Publication
Phylogenomic databases
eggNOGi | COG1309, Bacteria |
OMAi | MRVWART |
PhylomeDBi | P9WMC3 |
Family and domain databases
InterProi | View protein in InterPro IPR023772, DNA-bd_HTH_TetR-type_CS IPR009057, Homeobox-like_sf IPR001647, HTH_TetR |
Pfami | View protein in Pfam PF00440, TetR_N, 1 hit |
PRINTSi | PR00455, HTHTETR |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS01081, HTH_TETR_1, 1 hit PS50977, HTH_TETR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WMC3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTTRPATDRR KMPTGREEVA AAILQAATDL FAERGPAATS IRDIAARSKV
60 70 80 90 100
NHGLVFRHFG TKDQLVGAVL DHLGTKLTRL LHSEAPADII ERALDRHGRV
110 120 130 140 150
LARALLDGYP VGQLQQRFPN VAELLDAVRP RYDSDLGARL AVAHALALQF
160 170 180
GWRLFAPMLR SATGIDELTG DELRLSVNDA VARILEPH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP46227.1 |
PIRi | B70736 |
RefSeqi | NP_217922.1, NC_000962.3 WP_003417984.1, NZ_NVQJ01000027.1 |
Genome annotation databases
EnsemblBacteriai | CCP46227; CCP46227; Rv3405c |
GeneIDi | 23490322 887940 |
KEGGi | mtu:Rv3405c mtv:RVBD_3405c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP46227.1 |
PIRi | B70736 |
RefSeqi | NP_217922.1, NC_000962.3 WP_003417984.1, NZ_NVQJ01000027.1 |
3D structure databases
SMRi | P9WMC3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv3405c |
Proteomic databases
PaxDbi | P9WMC3 |
Genome annotation databases
EnsemblBacteriai | CCP46227; CCP46227; Rv3405c |
GeneIDi | 23490322 887940 |
KEGGi | mtu:Rv3405c mtv:RVBD_3405c |
Organism-specific databases
TubercuListi | Rv3405c |
Phylogenomic databases
eggNOGi | COG1309, Bacteria |
OMAi | MRVWART |
PhylomeDBi | P9WMC3 |
Family and domain databases
InterProi | View protein in InterPro IPR023772, DNA-bd_HTH_TetR-type_CS IPR009057, Homeobox-like_sf IPR001647, HTH_TetR |
Pfami | View protein in Pfam PF00440, TetR_N, 1 hit |
PRINTSi | PR00455, HTHTETR |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS01081, HTH_TETR_1, 1 hit PS50977, HTH_TETR_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HTHR_MYCTU | |
Accessioni | P9WMC3Primary (citable) accession number: P9WMC3 Secondary accession number(s): L0TE16, P67442, Q50720 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | December 2, 2020 | |
This is version 38 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |