UniProtKB - P9WKC3 (TGS4_MYCTU)
Protein
Probable diacyglycerol O-acyltransferase Tgs4
Gene
tgs4
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Required for maintaining the appropriate mycolic acid composition and permeability of the envelope on its exposure to acidic pH. Upon expression in E.coli functions as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has very weak wax synthase activity, incorporating palmityl alcohol into wax esters in the presence of palmitoyl-CoA.2 Publications
Catalytic activityi
- EC:2.3.1.20
pH dependencei
Optimum pH is 6.5-7.2.1 Publication
: triacylglycerol biosynthesis Pathwayi
This protein is involved in the pathway triacylglycerol biosynthesis, which is part of Glycerolipid metabolism.View all proteins of this organism that are known to be involved in the pathway triacylglycerol biosynthesis and in Glycerolipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 135 | Proton acceptorSequence analysis | 1 |
GO - Molecular functioni
- diacylglycerol O-acyltransferase activity Source: MTBBASE
- long-chain-alcohol O-fatty-acyltransferase activity Source: GO_Central
GO - Biological processi
- glycerolipid biosynthetic process Source: MTBBASE
- glycerol metabolic process Source: UniProtKB-KW
- growth of symbiont in host cell Source: MTBBASE
- response to acidic pH Source: MTBBASE
- response to hypoxia Source: GO_Central
- response to nitric oxide Source: MTBBASE
- triglyceride biosynthetic process Source: MTBBASE
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Glycerol metabolism, Lipid biosynthesis, Lipid metabolism |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-7350-MONOMER |
UniPathwayi | UPA00282 |
Chemistry databases
SwissLipidsi | SLP:000001149 |
Names & Taxonomyi
Protein namesi | Recommended name: Probable diacyglycerol O-acyltransferase Tgs4 (EC:2.3.1.20)Short name: TGS4 Alternative name(s): Probable triacylglycerol synthase tgs4 |
Gene namesi | Name:tgs4 Ordered Locus Names:Rv3088 ORF Names:MTV013.09 |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv3088 |
Pathology & Biotechi
Disruption phenotypei
Inactivation of the mymA operon causes altered cell wall structure, reduced contents and altered composition of mycolic acids along with the accumulation of saturated C24 and C26 fatty acids, and enhanced susceptibility to antibiotics, detergents and acidic pH. Also impairs ability to survive in macrophages.2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000222914 | 1 – 474 | Probable diacyglycerol O-acyltransferase Tgs4Add BLAST | 474 |
Proteomic databases
PaxDbi | P9WKC3 |
Expressioni
Inductioni
Expression is controlled by VirS. Induced at acidic pH and in macrophages. Induced by low levels of nitric oxide (NO), but not by hypoxia.2 Publications
Family & Domainsi
Sequence similaritiesi
Belongs to the long-chain O-acyltransferase family.Curated
Phylogenomic databases
KOi | K00635 |
OMAi | GHPLWEC |
PhylomeDBi | P9WKC3 |
Family and domain databases
InterProi | View protein in InterPro IPR014292 Acyl_transf_WS/DGAT IPR009721 O-acyltransferase_WSD1_C IPR004255 O-acyltransferase_WSD1_N |
Pfami | View protein in Pfam PF06974 DUF1298, 1 hit PF03007 WES_acyltransf, 1 hit |
TIGRFAMsi | TIGR02946 acyl_WS_DGAT, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WKC3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTRINPIDLS FLLLERANRP NHMAAYTIFE KPKGQKSSFG PRLFDAYRHS
60 70 80 90 100
QAAKPFNHKL KWLGTDVAAW ETVEPDMGYH IRHLALPAPG SMQQFHETVS
110 120 130 140 150
FLNTGLLDRG HPMWECYIID GIERGRIAIL LKVHHALIDG EGGLRAMRNF
160 170 180 190 200
LSDSPDDTTL AGPWMSAQGA DRPRRTPATV SRRAQLQGQL QGMIKGLTKL
210 220 230 240 250
PSGLFGVSAD AADLGAQALS LKARKASLPF TARRTLFNNT AKSAARAYGN
260 270 280 290 300
VELPLADVKA LAKATGTSVN DVVMTVIDDA LHHYLAEHQA STDRPLVAFM
310 320 330 340 350
PMSLREKSGE GGGNRVSAEL VPMGAPKASP VERLKEINAA TTRAKDKGRG
360 370 380 390 400
MQTTSRQAYA LLLLGSLTVA DALPLLGKLP SANVVISNMK GPTEQLYLAG
410 420 430 440 450
APLVAFSGLP IVPPGAGLNV TFASINTALC IAIGAAPEAV HEPSRLAELM
460 470
QRAFTELQTE AGTTSPTTSK SRTP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45897.1 |
PIRi | D70853 |
RefSeqi | NP_217604.1, NC_000962.3 WP_003416079.1, NZ_NVQJ01000011.1 |
Genome annotation databases
EnsemblBacteriai | CCP45897; CCP45897; Rv3088 |
GeneIDi | 888669 |
KEGGi | mtu:Rv3088 mtv:RVBD_3088 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45897.1 |
PIRi | D70853 |
RefSeqi | NP_217604.1, NC_000962.3 WP_003416079.1, NZ_NVQJ01000011.1 |
3D structure databases
SMRi | P9WKC3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv3088 |
Chemistry databases
SwissLipidsi | SLP:000001149 |
Proteomic databases
PaxDbi | P9WKC3 |
Genome annotation databases
EnsemblBacteriai | CCP45897; CCP45897; Rv3088 |
GeneIDi | 888669 |
KEGGi | mtu:Rv3088 mtv:RVBD_3088 |
Organism-specific databases
TubercuListi | Rv3088 |
Phylogenomic databases
KOi | K00635 |
OMAi | GHPLWEC |
PhylomeDBi | P9WKC3 |
Enzyme and pathway databases
UniPathwayi | UPA00282 |
BioCyci | MTBH37RV:G185E-7350-MONOMER |
Family and domain databases
InterProi | View protein in InterPro IPR014292 Acyl_transf_WS/DGAT IPR009721 O-acyltransferase_WSD1_C IPR004255 O-acyltransferase_WSD1_N |
Pfami | View protein in Pfam PF06974 DUF1298, 1 hit PF03007 WES_acyltransf, 1 hit |
TIGRFAMsi | TIGR02946 acyl_WS_DGAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TGS4_MYCTU | |
Accessioni | P9WKC3Primary (citable) accession number: P9WKC3 Secondary accession number(s): L0TBT5, O53305, P67208 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | September 18, 2019 | |
This is version 27 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways