UniProtKB - P9WJU7 (MMPL7_MYCTU)
Protein
Phthiocerol dimycocerosate exporter MmpL7
Gene
mmpL7
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Required for export of phthiocerol dimycocerosate (PDIM) to the cell wall (PubMed:10573420, PubMed:11279114). Essential for normal replication during the active-growth phase of the murine tuberculosis model (PubMed:15908378).3 Publications
Confers a high level of resistance to isoniazid (INH) when overexpressed in M.smegmatis.1 Publication
GO - Biological processi
- cell wall organization Source: UniProtKB-KW
- drug transmembrane transport Source: MTBBASE
- growth of symbiont in host cell Source: MTBBASE
- lipid transport Source: MTBBASE
- pathogenesis Source: UniProtKB-KW
- response to host immune response Source: MTBBASE
Keywordsi
Biological process | Cell wall biogenesis/degradation, Lipid transport, Transport, Virulence |
Enzyme and pathway databases
Reactomei | R-MTU-9635470, Dimycocersyl phthiocerol biosynthesis |
Names & Taxonomyi
Protein namesi | Recommended name: Phthiocerol dimycocerosate exporter MmpL7Curated |
Gene namesi | Name:mmpL7 Ordered Locus Names:Rv2942 ORF Names:MTCY24G1.07c |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv2942 |
Subcellular locationi
- Cell inner membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 44 – 64 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 210 – 230 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 241 – 261 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 271 – 291 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 311 – 331 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 344 – 364 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 389 – 409 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 761 – 781 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 790 – 810 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 822 – 842 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 864 – 884 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 888 – 908 | HelicalSequence analysisAdd BLAST | 21 |
GO - Cellular componenti
- integral component of plasma membrane Source: MTBBASE
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Disruption causes altered colony morphology (PubMed:10573420). Inactivation increases mouse survival (PubMed:15908378). Mutants are unable to liberate PDIM into the culture medium and are compromised for growth in mouse lungs, but not in the liver or spleen (PubMed:10573420). DIM are found mostly in the cytosol and plasma membrane, and mutants exhibit higher cell wall permeability and are more sensitive to detergent (PubMed:11279114).3 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 611 | I → S: Leads to decreased interaction with PpsE, but does not decrease MmpL7 activity in vivo. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000103570 | 1 – 920 | Phthiocerol dimycocerosate exporter MmpL7Add BLAST | 920 |
Post-translational modificationi
Phosphorylated by PknD in vitro. Phosphorylation could regulate the formation of the cell wall.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P9WJU7 |
Interactioni
Subunit structurei
Interacts with PpsE.
1 PublicationProtein-protein interaction databases
STRINGi | 83332.Rv2942 |
Family & Domainsi
Sequence similaritiesi
Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family. MmpL subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2409, Bacteria |
OMAi | VRQSMFS |
PhylomeDBi | P9WJU7 |
Family and domain databases
InterProi | View protein in InterPro IPR004869, MMPL_dom |
Pfami | View protein in Pfam PF03176, MMPL, 2 hits |
i Sequence
Sequence statusi: Complete.
P9WJU7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPSPAGRLHR IRYIRLKKSS PDCRATITSG SADGQRRSPR LTNLLVVAAW
60 70 80 90 100
VAAAVIANLL LTFTQAEPHD TSPALLPQDA KTAAATSRIA QAFPGTGSNA
110 120 130 140 150
IAYLVVEGGS TLEPQDQPYY DAAVGALRAD TRHVGSVLDW WSDPVTAPLG
160 170 180 190 200
TSPDGRSATA MVWLRGEAGT TQAAESLDAV RSVLRQLPPS EGLRASIVVP
210 220 230 240 250
AITNDMPMQI TAWQSATIVT VAAVIAVLLL LRARLSVRAA AIVLLTADLS
260 270 280 290 300
LAVAWPLAAV VRGHDWGTDS VFSWTLAAVL TIGTITAATM LAARLGSDAG
310 320 330 340 350
HSAAPTYRDS LPAFALPGAC VAIFTGPLLL ARTPALHGVG TAGLGVFVAL
360 370 380 390 400
AASLTVLPAL IALAGASRQL PAPTTGAGWT GRLSLPVSSA SALGTAAVLA
410 420 430 440 450
ICMLPIIGMR WGVAENPTRQ GGAQVLPGNA LPDVVVIKSA RDLRDPAALI
460 470 480 490 500
AINQVSHRLV EVPGVRKVES AAWPAGVPWT DASLSSAAGR LADQLGQQAG
510 520 530 540 550
SFVPAVTAIK SMKSIIEQMS GAVDQLDSTV NVTLAGARQA QQYLDPMLAA
560 570 580 590 600
ARNLKNKTTE LSEYLETIHT WIVGFTNCPD DVLCTAMRKV IEPYDIVVTG
610 620 630 640 650
MNELSTGADR ISAISTQTMS ALSSAPRMVA QMRSALAQVR SFVPKLETTI
660 670 680 690 700
QDAMPQIAQA SAMLKNLSAD FADTGEGGFH LSRKDLADPS YRHVRESMFS
710 720 730 740 750
SDGTATRLFL YSDGQLDLAA AARAQQLEIA AGKAMKYGSL VDSQVTVGGA
760 770 780 790 800
AQIAAAVRDA LIHDAVLLAV ILLTVVALAS MWRGAVHGAA VGVGVLASYL
810 820 830 840 850
AALGVSIALW QHLLDRELNA LVPLVSFAVL ASCGVPYLVA GIKAGRIADE
860 870 880 890 900
ATGARSKGAV SGRGAVAPLA ALGGVFGAGL VLVSGGSFSV LSQIGTVVVL
910 920
GLGVLITVQR AWLPTTPGRR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45745.1 |
PIRi | C70668 |
RefSeqi | NP_217458.1, NC_000962.3 WP_003414861.1, NZ_NVQJ01000015.1 |
Genome annotation databases
EnsemblBacteriai | CCP45745; CCP45745; Rv2942 |
GeneIDi | 23490817 887548 |
KEGGi | mtu:Rv2942 mtv:RVBD_2942 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45745.1 |
PIRi | C70668 |
RefSeqi | NP_217458.1, NC_000962.3 WP_003414861.1, NZ_NVQJ01000015.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 83332.Rv2942 |
Proteomic databases
PaxDbi | P9WJU7 |
Genome annotation databases
EnsemblBacteriai | CCP45745; CCP45745; Rv2942 |
GeneIDi | 23490817 887548 |
KEGGi | mtu:Rv2942 mtv:RVBD_2942 |
Organism-specific databases
TubercuListi | Rv2942 |
Phylogenomic databases
eggNOGi | COG2409, Bacteria |
OMAi | VRQSMFS |
PhylomeDBi | P9WJU7 |
Enzyme and pathway databases
Reactomei | R-MTU-9635470, Dimycocersyl phthiocerol biosynthesis |
Family and domain databases
InterProi | View protein in InterPro IPR004869, MMPL_dom |
Pfami | View protein in Pfam PF03176, MMPL, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MMPL7_MYCTU | |
Accessioni | P9WJU7Primary (citable) accession number: P9WJU7 Secondary accession number(s): L0TB36, P65370, P96289 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | December 2, 2020 | |
This is version 28 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families