UniProtKB - P9WJM9 (MSHC_MYCTU)
Protein
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase
Gene
mshC
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins.
Catalytic activityi
- 1D-myo-inositol 2-amino-2-deoxy-α-D-glucopyranoside + ATP + L-cysteine = 1D-myo-inositol 2-(L-cysteinylamino)-2-deoxy-α-D-glucopyranoside + AMP + diphosphate + H+1 PublicationEC:6.3.1.131 Publication
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 43 | ZincBy similarity | 1 | |
Binding sitei | 58 | Cysteinyl adenylateBy similarity | 1 | |
Binding sitei | 229 | Cysteinyl adenylateBy similarity | 1 | |
Metal bindingi | 233 | ZincBy similarity | 1 | |
Metal bindingi | 258 | ZincBy similarity | 1 | |
Binding sitei | 285 | Cysteinyl adenylate; via amide nitrogen and carbonyl oxygenBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: GO_Central
- cysteine-glucosaminylinositol ligase activity Source: MTBBASE
- cysteine-tRNA ligase activity Source: GO_Central
- zinc ion binding Source: UniProtKB-UniRule
GO - Biological processi
- cysteinyl-tRNA aminoacylation Source: GO_Central
- mycothiol biosynthetic process Source: MTBBASE
Keywordsi
Molecular function | Ligase |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-6336-MONOMER |
Reactomei | R-MTU-879299 Mycothiol biosynthesis |
Names & Taxonomyi
Protein namesi | Recommended name: L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (EC:6.3.1.13)Short name: L-Cys:GlcN-Ins ligase Alternative name(s): Mycothiol ligase Short name: MSH ligase |
Gene namesi | Name:mshC Synonyms:cysS2 Ordered Locus Names:Rv2130c ORF Names:MTCY261.29c |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv2130c |
Subcellular locationi
GO - Cellular componenti
- cytoplasm Source: GO_Central
- cytosol Source: GO_Central
- plasma membrane Source: MTBBASE
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000159442 | 1 – 414 | L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligaseAdd BLAST | 414 |
Proteomic databases
PaxDbi | P9WJM9 |
PRIDEi | P9WJM9 |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
STRINGi | 83332.Rv2130c |
Chemistry databases
BindingDBi | P9WJM9 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 43 – 46 | Cysteinyl adenylate bindingBy similarity | 4 | |
Regioni | 81 – 83 | Cysteinyl adenylate bindingBy similarity | 3 | |
Regioni | 251 – 253 | Cysteinyl adenylate bindingBy similarity | 3 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 45 – 55 | 'HIGH' regionAdd BLAST | 11 | |
Motifi | 189 – 194 | 'ERGGDP' region | 6 | |
Motifi | 291 – 295 | 'KMSKS' region | 5 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG4105C8N Bacteria COG0215 LUCA |
KOi | K15526 |
OMAi | YRSDWEW |
PhylomeDBi | P9WJM9 |
Family and domain databases
CDDi | cd00672 CysRS_core, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
HAMAPi | MF_01697 MshC, 1 hit |
InterProi | View protein in InterPro IPR024909 Cys-tRNA/MSH_ligase IPR017812 Mycothiol_ligase_MshC IPR014729 Rossmann-like_a/b/a_fold IPR032678 tRNA-synt_1_cat_dom |
PANTHERi | PTHR10890 PTHR10890, 1 hit PTHR10890:SF13 PTHR10890:SF13, 1 hit |
Pfami | View protein in Pfam PF01406 tRNA-synt_1e, 1 hit |
PRINTSi | PR00983 TRNASYNTHCYS |
TIGRFAMsi | TIGR03447 mycothiol_MshC, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WJM9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQSWYCPPVP VLPGRGPQLR LYDSADRQVR PVAPGSKATM YVCGITPYDA
60 70 80 90 100
THLGHAATYV TFDLIHRLWL DLGHELHYVQ NITDIDDPLF ERADRDGVDW
110 120 130 140 150
RDLAQAEVAL FCEDMAALRV LPPQDYVGAT EAIAEMVELI EKMLACGAAY
160 170 180 190 200
VIDREMGEYQ DIYFRADATL QFGYESGYDR DTMLRLCEER GGDPRRPGKS
210 220 230 240 250
DELDALLWRA ARPGEPSWPS PFGPGRPGWH VECAAIALSR IGSGLDIQGG
260 270 280 290 300
GSDLIFPHHE FTAAHAECVS GERRFARHYV HAGMIGWDGH KMSKSRGNLV
310 320 330 340 350
LVSALRAQDV EPSAVRLGLL AGHYRADRFW SQQVLDEATA RLHRWRTATA
360 370 380 390 400
LPAGPAAVDV VARVRRYLAD DLDTPKAIAA LDGWVTDAVE YGGHDAGAPK
410
LVATAIDALL GVDL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP44905.1 |
PIRi | E70514 |
RefSeqi | NP_216646.1, NC_000962.3 WP_003411091.1, NZ_NVQJ01000044.1 |
Genome annotation databases
EnsemblBacteriai | CCP44905; CCP44905; Rv2130c |
GeneIDi | 887492 |
KEGGi | mtu:Rv2130c mtv:RVBD_2130c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP44905.1 |
PIRi | E70514 |
RefSeqi | NP_216646.1, NC_000962.3 WP_003411091.1, NZ_NVQJ01000044.1 |
3D structure databases
SMRi | P9WJM9 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv2130c |
Chemistry databases
BindingDBi | P9WJM9 |
ChEMBLi | CHEMBL1744527 |
Proteomic databases
PaxDbi | P9WJM9 |
PRIDEi | P9WJM9 |
Genome annotation databases
EnsemblBacteriai | CCP44905; CCP44905; Rv2130c |
GeneIDi | 887492 |
KEGGi | mtu:Rv2130c mtv:RVBD_2130c |
Organism-specific databases
TubercuListi | Rv2130c |
Phylogenomic databases
eggNOGi | ENOG4105C8N Bacteria COG0215 LUCA |
KOi | K15526 |
OMAi | YRSDWEW |
PhylomeDBi | P9WJM9 |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-6336-MONOMER |
Reactomei | R-MTU-879299 Mycothiol biosynthesis |
Miscellaneous databases
PROi | PR:P9WJM9 |
Family and domain databases
CDDi | cd00672 CysRS_core, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
HAMAPi | MF_01697 MshC, 1 hit |
InterProi | View protein in InterPro IPR024909 Cys-tRNA/MSH_ligase IPR017812 Mycothiol_ligase_MshC IPR014729 Rossmann-like_a/b/a_fold IPR032678 tRNA-synt_1_cat_dom |
PANTHERi | PTHR10890 PTHR10890, 1 hit PTHR10890:SF13 PTHR10890:SF13, 1 hit |
Pfami | View protein in Pfam PF01406 tRNA-synt_1e, 1 hit |
PRINTSi | PR00983 TRNASYNTHCYS |
TIGRFAMsi | TIGR03447 mycothiol_MshC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MSHC_MYCTU | |
Accessioni | P9WJM9Primary (citable) accession number: P9WJM9 Secondary accession number(s): L0T8X7, O33264, P67017 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | October 16, 2019 | |
This is version 38 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names