UniProtKB - P9WIQ5 (RECC_MYCTU)
Protein
RecBCD enzyme subunit RecC
Gene
recC
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit recognizes the wild-type Chi sequence, and when added to isolated RecB increases its ATP-dependent helicase processivity.UniRule annotation
Catalytic activityi
- Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides.UniRule annotation EC:3.1.11.5
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- DNA binding Source: UniProtKB-UniRule
- DNA helicase activity Source: UniProtKB-UniRule
- exodeoxyribonuclease V activity Source: UniProtKB-EC
GO - Biological processi
- DNA recombination Source: GO_Central
- double-strand break repair via homologous recombination Source: UniProtKB-UniRule
Keywordsi
Molecular function | DNA-binding, Exonuclease, Helicase, Hydrolase, Nuclease |
Biological process | DNA damage, DNA repair |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-4769-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: RecBCD enzyme subunit RecCUniRule annotation (EC:3.1.11.5UniRule annotation)Alternative name(s): Exonuclease V subunit RecCUniRule annotation Short name: ExoV subunit RecCUniRule annotation Helicase/nuclease RecBCD subunit RecCUniRule annotation |
Gene namesi | Name:recCUniRule annotation Ordered Locus Names:Rv0631c ORF Names:MTCY20H10.12c |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv0631c |
Subcellular locationi
GO - Cellular componenti
- cell wall Source: MTBBASE
- exodeoxyribonuclease V complex Source: InterPro
- extracellular region Source: MTBBASE
- plasma membrane Source: MTBBASE
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000087122 | 1 – 1097 | RecBCD enzyme subunit RecCAdd BLAST | 1097 |
Proteomic databases
PaxDbi | P9WIQ5 |
PRIDEi | P9WIQ5 |
Interactioni
Subunit structurei
Heterotrimer of RecB, RecC and RecD. All subunits contribute to DNA-binding.
UniRule annotationProtein-protein interaction databases
STRINGi | 83332.Rv0631c |
Family & Domainsi
Sequence similaritiesi
Belongs to the RecC family.UniRule annotation
Phylogenomic databases
eggNOGi | ENOG4105DSY Bacteria COG1330 LUCA |
KOi | K03583 |
OMAi | MCPDIET |
PhylomeDBi | P9WIQ5 |
Family and domain databases
Gene3Di | 1.10.10.160, 1 hit |
HAMAPi | MF_01486 RecC, 1 hit |
InterProi | View protein in InterPro IPR013986 DExx_box_DNA_helicase_dom_sf IPR027417 P-loop_NTPase IPR006697 RecC IPR041500 RecC_C IPR011335 Restrct_endonuc-II-like |
Pfami | View protein in Pfam PF17946 RecC_C, 1 hit |
PIRSFi | PIRSF000980 RecC, 1 hit |
SUPFAMi | SSF52540 SSF52540, 2 hits SSF52980 SSF52980, 1 hit |
TIGRFAMsi | TIGR01450 recC, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WIQ5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALHLHRAER TDLLADGLGA LLADPQPDPF AQELVLVAAR GVERWLSQRL
60 70 80 90 100
SLVLGCGPGR ADGVCAGIAF RNPQSLIAEI TGTLDDDPWS PEALAWPLLA
110 120 130 140 150
VIDASLDEPW CRTLASHLGH FATTDAEAEL RRGRRYSVAR RLAGLFASYA
160 170 180 190 200
RQRPGLLAAW LDGDLGELPG DLAWQPPLWR ALVTTVGADP PHVRHDKTIA
210 220 230 240 250
RLRDGPADLP ARLSLFGHTR LACTDVQLLD ALAVHHDLHL WLPHPSDELW
260 270 280 290 300
RALAGFQGAD GLLPRRQDTS RRAAQHPLLE TLGRDVRELQ RALPAARATD
310 320 330 340 350
EFLGATTKPD TLLGWLQADI AGNAPRPAGR SLSDADRSVQ VHACHGPARQ
360 370 380 390 400
IDVLREVLLG LLEDDPTLQP RDIVVMCPDI DTYAPLIVAG FGLGEVAGDC
410 420 430 440 450
HPAHRLRVRL ADRALTQTNP LLSVAAELLT IAETRATASQ LLNLAQAAPV
460 470 480 490 500
RAKFGFADDD LDTITTWVRE SNIRWGFDPT HRRRYGLDTV VHNTWRFGLD
510 520 530 540 550
RILTGVAMSE DSQAWLDTAL PLDDVGSNRV ELAGRLAEFV ERLHHVVGGL
560 570 580 590 600
SGARPLVAWL DALATGIDLL TACNDGWQRA QVQREFADVL ARAGSRAAPL
610 620 630 640 650
LRLPDVRALL DAQLAGRPTR ANFRTGTLTV CTMVPMRSVP HRVVCLVGLD
660 670 680 690 700
DGVFPRLSHP DGDDVLAREP MTGERDIRSE DRQLLLDAIG AATQTLVITY
710 720 730 740 750
TGADERTGQP RPPAVPLAEL LDALDQTTSA PVRERILVTH PLQPFDRKNV
760 770 780 790 800
TPGALLGAKP FTFDPAALAA AQAAAGKRCP PTAFISGRLP APPAADVTLA
810 820 830 840 850
DLLDFFKDPV KGFFRALDYT LPWDVDTVED SIPVQVDALA EWTVGERMLR
860 870 880 890 900
DMLRGLHPDD AAHSEWRRGT LPPGRLGVRR AKEIRNRARD LAAAALAHRD
910 920 930 940 950
GHGQAHDVDV DLGDGRRLSG TVTPVFGGRT VSVTYSKLAP KHVLPAWIGL
960 970 980 990 1000
VTLAAQEPGR EWSALCIGRS KTRNHIARRL FVPPPDPVAV LRELVLLYDA
1010 1020 1030 1040 1050
GRREPLPLPL KTSCAWAQAR RDGQDPYPPA RECWQTNRFR PGDDDAPAHV
1060 1070 1080 1090
RAWGPRAPFE VLLGKPRAGE EVAGEETRLG ALAARLWLPL LAAEGSV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP43372.1 |
PIRi | D70612 |
RefSeqi | NP_215145.1, NC_000962.3 WP_003900193.1, NZ_KK339370.1 |
Genome annotation databases
EnsemblBacteriai | CCP43372; CCP43372; Rv0631c |
GeneIDi | 888008 |
KEGGi | mtu:Rv0631c mtv:RVBD_0631c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP43372.1 |
PIRi | D70612 |
RefSeqi | NP_215145.1, NC_000962.3 WP_003900193.1, NZ_KK339370.1 |
3D structure databases
SMRi | P9WIQ5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv0631c |
Proteomic databases
PaxDbi | P9WIQ5 |
PRIDEi | P9WIQ5 |
Genome annotation databases
EnsemblBacteriai | CCP43372; CCP43372; Rv0631c |
GeneIDi | 888008 |
KEGGi | mtu:Rv0631c mtv:RVBD_0631c |
Organism-specific databases
TubercuListi | Rv0631c |
Phylogenomic databases
eggNOGi | ENOG4105DSY Bacteria COG1330 LUCA |
KOi | K03583 |
OMAi | MCPDIET |
PhylomeDBi | P9WIQ5 |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-4769-MONOMER |
Family and domain databases
Gene3Di | 1.10.10.160, 1 hit |
HAMAPi | MF_01486 RecC, 1 hit |
InterProi | View protein in InterPro IPR013986 DExx_box_DNA_helicase_dom_sf IPR027417 P-loop_NTPase IPR006697 RecC IPR041500 RecC_C IPR011335 Restrct_endonuc-II-like |
Pfami | View protein in Pfam PF17946 RecC_C, 1 hit |
PIRSFi | PIRSF000980 RecC, 1 hit |
SUPFAMi | SSF52540 SSF52540, 2 hits SSF52980 SSF52980, 1 hit |
TIGRFAMsi | TIGR01450 recC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RECC_MYCTU | |
Accessioni | P9WIQ5Primary (citable) accession number: P9WIQ5 Secondary accession number(s): L0T7A5, P96921 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | October 16, 2019 | |
This is version 34 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names