P9WII3 · MAZF6_MYCTU

Function

function

Toxic component of a type II toxin-antitoxin (TA) system. Upon expression in E.coli and in M.smegmatis partially inhibits cell growth and colony formation; its toxic effect is neutralized by coexpression with cognate antitoxin MazE6. Acts as an mRNA interferase on ssRNA, cleaving between the second and third bases in the sequences CUCCU and UUCCU (PubMed:18485066).
Further experiments demonstrate that it digests between the first and second bases of UCCUU, yielding a 5'-hydroxyl end; digests M.tuberculosis mRNA (in coding as well as the 5'- and 3'-UTR regions) and 23S rRNA, digests E.coli 16S rRNA both alone and in the 70S ribosome but no data for M.tuberculosis 16S rRNA cleavage was presented. 23S and 16S rRNA digestion occurs in predicted single-stranded regions, the 16S rRNA UCCUU site is in the anti-Shine-Dalgarno site and would cleave off the last 7 nucleotides (PubMed:24709835).
Non-cognate antitoxins VapB27 and VapB40 partially neutralize toxicity in vivo (PubMed:20876537).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentextracellular region
Molecular FunctionDNA binding
Molecular FunctionRNA endonuclease activity
Molecular FunctionRNA nuclease activity
Biological Processmodulation by symbiont of host process
Biological ProcessmRNA catabolic process
Biological Processnegative regulation of growth
Biological Processpositive regulation of growth
Biological ProcessrRNA catabolic process

Keywords

Enzyme and pathway databases

Community curation (1)

Analysis of paired-end RNA sequencing data revealed that the over expression of MazF6 resulted in differential expression of 323 transcripts in M. tuberculosis. The transcription profiles obtained in M. tuberculosis upon over expression of MazF6, were similar to those obtained from bacteria exposed to stress conditions such as nutrient starvation, enduring hypoxic response (EHR), non-replicating persisters (NRP), and drug-induced persisters. Toxin over expression led to the up regulation of transcripts involved in cell wall and cell processes, intermediary metabolism and respiration.

Names & Taxonomy

Protein names

  • Recommended name
    Endoribonuclease MazF6
  • EC number
  • Alternative names
    • Toxin MazF6
    • mRNA interferase MazF-mt3

Gene names

    • Name
      mazF6
    • Synonyms
      mazF-mt3
    • ORF names
      MTCY39.28
    • Ordered locus names
      Rv1991c
Community curation (1)

Organism names

Accessions

  • Primary accession
    P9WII3
  • Secondary accessions
    • L0T8B0
    • P64911
    • Q10867

Proteomes

Organism-specific databases

Subcellular Location

Note: Following 6 weeks of nutrient starvation.

Keywords

Phenotypes & Variants

Disruption phenotype

Individual deletion of mazF3, mazF6 and mazF9 have little to no phenotype, but a triple mutant shows increased sensitivity to oxidative and antibiotic stress and starvation, decreased formation of persisters cells, and a decreased bacterial load and pathogenic damage in infected guinea pigs.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002019041-114Endoribonuclease MazF6

Proteomic databases

Expression

Induction

Strongly induced (17 to 25-fold) by nitrosative stress, starvation, when grown in a non-replicating state, in the presence of gentamycin or rifampicin.

Interaction

Subunit

Forms a complex with cognate toxin MazE6, which neutralizes the toxin. Interacts physically with non-cognate antitoxins VapB27 and VapB40 which neutralize the toxin.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the PemK/MazF family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    114
  • Mass (Da)
    12,262
  • Last updated
    2014-04-16 v1
  • Checksum
    59A18C54B9EDDBCE
MVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTAIVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL123456
EMBL· GenBank· DDBJ
CCP44762.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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