P9WII3 · MAZF6_MYCTU
- ProteinEndoribonuclease MazF6
- GenemazF6
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids114 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Toxic component of a type II toxin-antitoxin (TA) system. Upon expression in E.coli and in M.smegmatis partially inhibits cell growth and colony formation; its toxic effect is neutralized by coexpression with cognate antitoxin MazE6. Acts as an mRNA interferase on ssRNA, cleaving between the second and third bases in the sequences CUCCU and UUCCU (PubMed:18485066).
Further experiments demonstrate that it digests between the first and second bases of UCCUU, yielding a 5'-hydroxyl end; digests M.tuberculosis mRNA (in coding as well as the 5'- and 3'-UTR regions) and 23S rRNA, digests E.coli 16S rRNA both alone and in the 70S ribosome but no data for M.tuberculosis 16S rRNA cleavage was presented. 23S and 16S rRNA digestion occurs in predicted single-stranded regions, the 16S rRNA UCCUU site is in the anti-Shine-Dalgarno site and would cleave off the last 7 nucleotides (PubMed:24709835).
Non-cognate antitoxins VapB27 and VapB40 partially neutralize toxicity in vivo (PubMed:20876537).
Further experiments demonstrate that it digests between the first and second bases of UCCUU, yielding a 5'-hydroxyl end; digests M.tuberculosis mRNA (in coding as well as the 5'- and 3'-UTR regions) and 23S rRNA, digests E.coli 16S rRNA both alone and in the 70S ribosome but no data for M.tuberculosis 16S rRNA cleavage was presented. 23S and 16S rRNA digestion occurs in predicted single-stranded regions, the 16S rRNA UCCUU site is in the anti-Shine-Dalgarno site and would cleave off the last 7 nucleotides (PubMed:24709835).
Non-cognate antitoxins VapB27 and VapB40 partially neutralize toxicity in vivo (PubMed:20876537).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | DNA binding | |
Molecular Function | RNA endonuclease activity | |
Molecular Function | RNA nuclease activity | |
Biological Process | modulation by symbiont of host process | |
Biological Process | mRNA catabolic process | |
Biological Process | negative regulation of growth | |
Biological Process | positive regulation of growth | |
Biological Process | rRNA catabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Analysis of paired-end RNA sequencing data revealed that the over expression of MazF6 resulted in differential expression of 323 transcripts in M. tuberculosis. The transcription profiles obtained in M. tuberculosis upon over expression of MazF6, were similar to those obtained from bacteria exposed to stress conditions such as nutrient starvation, enduring hypoxic response (EHR), non-replicating persisters (NRP), and drug-induced persisters. Toxin over expression led to the up regulation of transcripts involved in cell wall and cell processes, intermediary metabolism and respiration.
Names & Taxonomy
Protein names
- Recommended nameEndoribonuclease MazF6
- EC number
- Alternative names
Gene names
- Community suggested namesMazF6; mazF6.
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium tuberculosis complex
Accessions
- Primary accessionP9WII3
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Following 6 weeks of nutrient starvation.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Individual deletion of mazF3, mazF6 and mazF9 have little to no phenotype, but a triple mutant shows increased sensitivity to oxidative and antibiotic stress and starvation, decreased formation of persisters cells, and a decreased bacterial load and pathogenic damage in infected guinea pigs.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000201904 | 1-114 | Endoribonuclease MazF6 | |||
Sequence: MVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTAIVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL |
Proteomic databases
Expression
Induction
Strongly induced (17 to 25-fold) by nitrosative stress, starvation, when grown in a non-replicating state, in the presence of gentamycin or rifampicin.
Interaction
Subunit
Forms a complex with cognate toxin MazE6, which neutralizes the toxin. Interacts physically with non-cognate antitoxins VapB27 and VapB40 which neutralize the toxin.
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length114
- Mass (Da)12,262
- Last updated2014-04-16 v1
- Checksum59A18C54B9EDDBCE
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL123456 EMBL· GenBank· DDBJ | CCP44762.1 EMBL· GenBank· DDBJ | Genomic DNA |