UniProtKB - P9WI71 (PKNH_MYCTU)
Protein
Serine/threonine-protein kinase PknH
Gene
pknH
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
May regulate bacterial growth in response to external signals to facilitate adaptation to the host environment. In vitro, phosphorylates several substrates such as EmbR, DevR (DosR), DacB1 and Rv0681.7 Publications
Catalytic activityi
Cofactori
a divalent metal cation1 Publication
Activity regulationi
Inhibited by the kinase inhibitors staurosporine and H-7.1 Publication
Kineticsi
- KM=0.597 µM for EmbR1 Publication
- KM=1.59 µM for DacB11 Publication
- KM=19.82 µM for Rv06811 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 45 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 139 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 22 – 30 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- protein kinase activity Source: MTBBASE
- protein serine/threonine kinase activity Source: MTBBASE
GO - Biological processi
- negative regulation of catalytic activity Source: MTBBASE
- negative regulation of growth Source: MTBBASE
- positive regulation of catalytic activity Source: MTBBASE
- positive regulation of DNA binding Source: MTBBASE
- positive regulation of transcription, DNA-templated Source: MTBBASE
- protein autophosphorylation Source: MTBBASE
- regulation of lipid biosynthetic process Source: MTBBASE
- response to host immune response Source: MTBBASE
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Stress response |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
SABIO-RKi | P9WI71 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:pknH Ordered Locus Names:Rv1266c ORF Names:MTCY50.16 |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv1266c |
Subcellular locationi
- Cell membrane 1 Publication; Single-pass membrane protein 1 Publication
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 403 | CytoplasmicSequence analysisAdd BLAST | 403 | |
Transmembranei | 404 – 424 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 425 – 626 | ExtracellularSequence analysisAdd BLAST | 202 |
GO - Cellular componenti
- cell wall Source: MTBBASE
- integral component of plasma membrane Source: MTBBASE
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Deletion causes hypervirulence during the chronic phase of infection in BALB/c mice. Mutant displays increased resistance to acidified nitrite stress. Does not affect sensitivity to ethambutol.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 45 | K → M: Abolished kinase activity. 2 Publications | 1 | |
Mutagenesisi | 170 | T → A: Abolished autophosphorylation. 1 Publication | 1 |
Miscellaneous databases
PHI-basei | PHI:3624 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000171218 | 1 – 626 | Serine/threonine-protein kinase PknHAdd BLAST | 626 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 170 | Phosphothreonine1 Publication | 1 | |
Disulfide bondi | 482 ↔ 545 | 1 Publication | ||
Disulfide bondi | 587 ↔ 604 | 1 Publication |
Post-translational modificationi
Autophosphorylated on threonine and serine residues. Dephosphorylated by PstP.1 Publication
Keywords - PTMi
Disulfide bond, PhosphoproteinProteomic databases
PaxDbi | P9WI71 |
PRIDEi | P9WI71 |
PTM databases
iPTMneti | P9WI71 |
Expressioni
Inductioni
Repressed by low pH and heat shock. Up-regulated inside the host macrophages.2 Publications
Interactioni
Protein-protein interaction databases
IntActi | P9WI71, 2 interactors |
STRINGi | 83332.Rv1266c |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P9WI71 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 16 – 276 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 261 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 297 – 403 | Pro-richAdd BLAST | 107 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0515, Bacteria |
OMAi | MTTPYIV |
Family and domain databases
Gene3Di | 3.40.1000.70, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR026954, PknH-like_Extracell IPR038232, PknH-like_Extracell_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit PF14032, PknH_C, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WI71-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSDAQDSRVG SMFGPYHLKR LLGRGGMGEV YEAEHTVKEW TVAVKLMTAE
60 70 80 90 100
FSKDPVFRER MKREARIAGR LQEPHVVPIH DYGEVDGQMF LEMRLVEGTD
110 120 130 140 150
LDSVLKRFGP LTPPRAVAII TQIASALDAA HADGVMHRDV KPQNILITRD
160 170 180 190 200
DFAYLVDFGI ASATTDEKLT QLGTAVGTWK YMAPERFSND EVTYRADIYA
210 220 230 240 250
LACVLHECLT GAPPYRADSA GTLVSSHLMG PIPQPSAIRP GIPKAFDAVV
260 270 280 290 300
ARGMAKKPED RYASAGDLAL AAHEALSDPD QDHAADILRR SQESTLPAPP
310 320 330 340 350
KPVPPPTMPA TAMAPRQPPA PPVTPPGVQP APKPSYTPPA QPGPAGQRPG
360 370 380 390 400
PTGQPSWAPN SGPMPASGPT PTPQYYQGGG WGAPPSGGPS PWAQTPRKTN
410 420 430 440 450
PWPLVAGAAA VVLVLVLGAI GIWIAIRPKP VQPPQPVAEE RLSALLLNSS
460 470 480 490 500
EVNAVMGSSS MQPGKPITSM DSSPVTVSLP DCQGALYTSQ DPVYAGTGYT
510 520 530 540 550
AINGLISSEP GDNYEHWVNQ AVVAFPTADK ARAFVQTSAD KWKNCAGKTV
560 570 580 590 600
TVTNKAKTYR WTFADVKGSP PTITVIDTQE GAEGWECQRA MSVANNVVVD
610 620
VNACGYRITN QAGQIAAKIV DKVNKE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP44022.1 |
PIRi | B70754 |
RefSeqi | NP_215782.1, NC_000962.3 WP_010886110.1, NC_000962.3 |
Genome annotation databases
EnsemblBacteriai | CCP44022; CCP44022; Rv1266c |
GeneIDi | 887023 |
KEGGi | mtu:Rv1266c |
PATRICi | fig|83332.12.peg.1418 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP44022.1 |
PIRi | B70754 |
RefSeqi | NP_215782.1, NC_000962.3 WP_010886110.1, NC_000962.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4ESQ | X-ray | 1.70 | A | 435-626 | [»] | |
SMRi | P9WI71 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P9WI71, 2 interactors |
STRINGi | 83332.Rv1266c |
PTM databases
iPTMneti | P9WI71 |
Proteomic databases
PaxDbi | P9WI71 |
PRIDEi | P9WI71 |
Genome annotation databases
EnsemblBacteriai | CCP44022; CCP44022; Rv1266c |
GeneIDi | 887023 |
KEGGi | mtu:Rv1266c |
PATRICi | fig|83332.12.peg.1418 |
Organism-specific databases
TubercuListi | Rv1266c |
Phylogenomic databases
eggNOGi | COG0515, Bacteria |
OMAi | MTTPYIV |
Enzyme and pathway databases
SABIO-RKi | P9WI71 |
Miscellaneous databases
PHI-basei | PHI:3624 |
Family and domain databases
Gene3Di | 3.40.1000.70, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR026954, PknH-like_Extracell IPR038232, PknH-like_Extracell_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit PF14032, PknH_C, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PKNH_MYCTU | |
Accessioni | P9WI71Primary (citable) accession number: P9WI71 Secondary accession number(s): L0T6C9, Q11053 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | December 2, 2020 | |
This is version 41 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families