UniProtKB - P9WFE7 (URE3_MYCTU)
Protein
Urease subunit gamma
Gene
ureA
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
Catalytic activityi
- EC:3.5.1.5UniRule annotation1 Publication
Kineticsi
- KM=0.3 mM for urea1 Publication
- Vmax=0.05 mmol/min/mg enzyme1 Publication
pH dependencei
Optimum pH is 7.2.1 Publication
: urea degradation Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotationProteins known to be involved in this subpathway in this organism are:
- Urease subunit alpha (ureC), Urease subunit gamma (ureA), Urease subunit beta (ureB)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.
GO - Molecular functioni
- nickel cation binding Source: InterPro
- urease activity Source: UniProtKB-UniRule
GO - Biological processi
- urea catabolic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Hydrolase |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-6043-MONOMER |
UniPathwayi | UPA00258;UER00370 |
Names & Taxonomyi
Protein namesi | Recommended name: Urease subunit gammaUniRule annotation (EC:3.5.1.5UniRule annotation)Alternative name(s): Urea amidohydrolase subunit gammaUniRule annotation |
Gene namesi | Name:ureAUniRule annotation Ordered Locus Names:Rv1848 ORF Names:MTCY359.25c |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv1848 |
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000098019 | 1 – 100 | Urease subunit gammaAdd BLAST | 100 |
Proteomic databases
PaxDbi | P9WFE7 |
Interactioni
Subunit structurei
Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.
Protein-protein interaction databases
STRINGi | 83332.Rv1848 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P9WFE7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the urease gamma subunit family.UniRule annotation
Phylogenomic databases
eggNOGi | ENOG4108YZ9 Bacteria COG0831 LUCA |
KOi | K01430 |
OMAi | QLTPHEQ |
PhylomeDBi | P9WFE7 |
Family and domain databases
CDDi | cd00390 Urease_gamma, 1 hit |
Gene3Di | 3.30.280.10, 1 hit |
HAMAPi | MF_00739 Urease_gamma, 1 hit |
InterProi | View protein in InterPro IPR012010 Urease_gamma IPR002026 Urease_gamma/gamma-beta_su IPR036463 Urease_gamma_sf |
Pfami | View protein in Pfam PF00547 Urease_gamma, 1 hit |
PIRSFi | PIRSF001223 Urease_gamma, 1 hit |
SUPFAMi | SSF54111 SSF54111, 1 hit |
TIGRFAMsi | TIGR00193 urease_gam, 1 hit |
i Sequence
Sequence statusi: Complete.
P9WFE7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRLTPHEQER LLLSYAAELA RRRRARGLRL NHPEAIAVIA DHILEGARDG
60 70 80 90 100
RTVAELMASG REVLGRDDVM EGVPEMLAEV QVEATFPDGT KLVTVHQPIA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 75 | E → V in AAC37005 (PubMed:7568014).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U33011 Genomic DNA Translation: AAC43473.1 L41141 Genomic DNA Translation: AAC37005.1 AL123456 Genomic DNA Translation: CCP44614.1 |
PIRi | H70664 |
RefSeqi | NP_216364.1, NC_000962.3 WP_003409305.1, NZ_NVQJ01000013.1 |
Genome annotation databases
EnsemblBacteriai | CCP44614; CCP44614; Rv1848 |
GeneIDi | 885414 |
KEGGi | mtu:Rv1848 mtv:RVBD_1848 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U33011 Genomic DNA Translation: AAC43473.1 L41141 Genomic DNA Translation: AAC37005.1 AL123456 Genomic DNA Translation: CCP44614.1 |
PIRi | H70664 |
RefSeqi | NP_216364.1, NC_000962.3 WP_003409305.1, NZ_NVQJ01000013.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2FVH | X-ray | 1.80 | A/B/C | 1-100 | [»] | |
SMRi | P9WFE7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv1848 |
Proteomic databases
PaxDbi | P9WFE7 |
Genome annotation databases
EnsemblBacteriai | CCP44614; CCP44614; Rv1848 |
GeneIDi | 885414 |
KEGGi | mtu:Rv1848 mtv:RVBD_1848 |
Organism-specific databases
TubercuListi | Rv1848 |
Phylogenomic databases
eggNOGi | ENOG4108YZ9 Bacteria COG0831 LUCA |
KOi | K01430 |
OMAi | QLTPHEQ |
PhylomeDBi | P9WFE7 |
Enzyme and pathway databases
UniPathwayi | UPA00258;UER00370 |
BioCyci | MTBH37RV:G185E-6043-MONOMER |
Family and domain databases
CDDi | cd00390 Urease_gamma, 1 hit |
Gene3Di | 3.30.280.10, 1 hit |
HAMAPi | MF_00739 Urease_gamma, 1 hit |
InterProi | View protein in InterPro IPR012010 Urease_gamma IPR002026 Urease_gamma/gamma-beta_su IPR036463 Urease_gamma_sf |
Pfami | View protein in Pfam PF00547 Urease_gamma, 1 hit |
PIRSFi | PIRSF001223 Urease_gamma, 1 hit |
SUPFAMi | SSF54111 SSF54111, 1 hit |
TIGRFAMsi | TIGR00193 urease_gam, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | URE3_MYCTU | |
Accessioni | P9WFE7Primary (citable) accession number: P9WFE7 Secondary accession number(s): L0TAS5, P0A676, P50043 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 16, 2014 |
Last sequence update: | April 16, 2014 | |
Last modified: | October 16, 2019 | |
This is version 31 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references