UniProtKB - P96264 (LPQL_MYCTU)
Protein
Probable lipoprotein aminopeptidase LpqL
Gene
lpqL
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
An aminopeptidase; acts on free N-terminal amino groups with a very strong preference for Leu in the first position.By similarity
Catalytic activityi
- Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.By similarity EC:3.4.11.1
Cofactori
Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 271 | Zinc 1; catalyticBy similarity | 1 | |
Metal bindingi | 283 | Zinc 1; catalyticBy similarity | 1 | |
Metal bindingi | 283 | Zinc 2; catalyticBy similarity | 1 | |
Active sitei | 316 | Proton acceptorBy similarity | 1 | |
Metal bindingi | 317 | Zinc 2; catalyticBy similarity | 1 | |
Metal bindingi | 345 | Zinc 1; catalyticBy similarity | 1 | |
Sitei | 447 | Transition state stabilizerBy similarity | 1 | |
Metal bindingi | 448 | Zinc 2; catalyticBy similarity | 1 |
GO - Molecular functioni
- aminopeptidase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- proteolysis Source: GO_Central
Keywordsi
Molecular function | Aminopeptidase, Hydrolase, Protease |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-4543-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Probable lipoprotein aminopeptidase LpqL1 Publication (EC:3.4.11.1By similarity)Alternative name(s): Leucine aminopeptidase Lipoprotein LpqL |
Gene namesi | Name:lpqL Ordered Locus Names:Rv0418 ORF Names:LH57_02235 |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv0418 |
Subcellular locationi
- Cell membrane PROSITE-ProRule annotation; Lipid-anchor PROSITE-ProRule annotation1 Publication
GO - Cellular componenti
- extracellular region Source: MTBBASE
- plasma membrane Source: MTBBASE
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | PROSITE-ProRule annotationAdd BLAST | 24 | |
ChainiPRO_0000434901 | 25 – 500 | Probable lipoprotein aminopeptidase LpqLPROSITE-ProRule annotation1 PublicationAdd BLAST | 476 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 25 | N-palmitoyl cysteine1 Publication | 1 | |
Lipidationi | 25 | S-diacylglycerol cysteine1 Publication | 1 |
Post-translational modificationi
Modified by Lgt on Cys-25 with an S-linked diacylglycerol with a mixture of C16 and C19 fatty acids (palmitic and tuberculostearic acid), signal peptide is removed by LspA, modified by Lnt with an amide-linked mixture of C16 and C19 fatty acids, expressed in M.bovis (PubMed:24093492).1 Publication
Keywords - PTMi
Lipoprotein, PalmitateProteomic databases
PaxDbi | P96264 |
PRIDEi | P96264 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 140 – 231 | PASequence analysisAdd BLAST | 92 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG4105IIU Bacteria COG2234 LUCA |
HOGENOMi | HOG000051000 |
OMAi | YGHPTRV |
PhylomeDBi | P96264 |
Family and domain databases
CDDi | cd03876 M28_SGAP_like, 1 hit |
InterProi | View protein in InterPro IPR041756 M28_SGAP-like IPR003137 PA_domain IPR007484 Peptidase_M28 |
Pfami | View protein in Pfam PF02225 PA, 1 hit PF04389 Peptidase_M28, 1 hit |
PROSITEi | View protein in PROSITE PS51257 PROKAR_LIPOPROTEIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P96264-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVNKSRMMPA VLAVAVVVAF LTTGCIRWST QSRPVVNGPA AAEFAVALRN
60 70 80 90 100
RVSTDAMMAH LSKLQDIANA NDGTRAVGTP GYQASVDYVV NTLRNSGFDV
110 120 130 140 150
QTPEFSARVF KAEKGVVTLG GNTVEARALE YSLGTPPDGV TGPLVAAPAD
160 170 180 190 200
DSPGCSPSDY DRLPVSGAVV LVDRGVCPFA QKEDAAAQRG AVALIIADNI
210 220 230 240 250
DEQAMGGTLG ANTDVKIPVV SVTKSVGFQL RGQSGPTTVK LTASTQSFKA
260 270 280 290 300
RNVIAQTKTG SSANVVMAGA HLDSVPEGPG INDNGSGVAA VLETAVQLGN
310 320 330 340 350
SPHVSNAVRF AFWGAEEFGL IGSRNYVESL DIDALKGIAL YLNFDMLASP
360 370 380 390 400
NPGYFTYDGD QSLPLDARGQ PVVPEGSAGI ERTFVAYLKM AGKTAQDTSF
410 420 430 440 450
DGRSDYDGFT LAGIPSGGLF SGAEVKKSAE QAELWGGTAD EPFDPNYHQK
460 470 480 490 500
TDTLDHIDRT ALGINGAGVA YAVGLYAQDL GGPNGVPVMA DRTRHLIAKP
Mass spectrometryi
Molecular mass is 54200 Da from positions 25 - 500. Determined by MALDI. Expressed in M.bovis, lipidated.1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP009480 Genomic DNA Translation: AIR13138.1 AL123456 Genomic DNA Translation: CCP43149.1 |
RefSeqi | NP_214932.1, NC_000962.3 WP_003402133.1, NZ_NVQJ01000002.1 |
Genome annotation databases
EnsemblBacteriai | AIR13138; AIR13138; LH57_02235 CCP43149; CCP43149; Rv0418 |
GeneIDi | 886381 |
KEGGi | mtu:Rv0418 mtv:RVBD_0418 |
PATRICi | fig|83332.111.peg.459 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP009480 Genomic DNA Translation: AIR13138.1 AL123456 Genomic DNA Translation: CCP43149.1 |
RefSeqi | NP_214932.1, NC_000962.3 WP_003402133.1, NZ_NVQJ01000002.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 83332.Rv0418 |
Proteomic databases
PaxDbi | P96264 |
PRIDEi | P96264 |
Genome annotation databases
EnsemblBacteriai | AIR13138; AIR13138; LH57_02235 CCP43149; CCP43149; Rv0418 |
GeneIDi | 886381 |
KEGGi | mtu:Rv0418 mtv:RVBD_0418 |
PATRICi | fig|83332.111.peg.459 |
Organism-specific databases
TubercuListi | Rv0418 |
Phylogenomic databases
eggNOGi | ENOG4105IIU Bacteria COG2234 LUCA |
HOGENOMi | HOG000051000 |
OMAi | YGHPTRV |
PhylomeDBi | P96264 |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-4543-MONOMER |
Family and domain databases
CDDi | cd03876 M28_SGAP_like, 1 hit |
InterProi | View protein in InterPro IPR041756 M28_SGAP-like IPR003137 PA_domain IPR007484 Peptidase_M28 |
Pfami | View protein in Pfam PF02225 PA, 1 hit PF04389 Peptidase_M28, 1 hit |
PROSITEi | View protein in PROSITE PS51257 PROKAR_LIPOPROTEIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LPQL_MYCTU | |
Accessioni | P96264Primary (citable) accession number: P96264 Secondary accession number(s): F2GMD5, I6XV80, L0T5B2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 9, 2015 |
Last sequence update: | May 1, 1997 | |
Last modified: | October 16, 2019 | |
This is version 114 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names