P93324 · CHOMT_MEDSA

  • Protein
    Isoliquiritigenin 2'-O-methyltransferase
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Methylates the 2'-hydroxyl of isoliquiritigenin and licodione. Does not methylate narigenin chalcone, caffeic acid or daidzein. Involved in the root nodulation initiation by promoting the biosynthesis of nod-inducing molecules.

Catalytic activity

Activity regulation

Inhibited by 1 mM Co2+, Cu2+, Zn2+ or Fe2+. Non-competitively inhibited by S-adenosyl-L-homocysteine. Competitively inhibited by 2'-O-methylisoliquiritigenin.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
2.2 μMisoliquiritigenin
17.7 μMS-adenosyl-L-methionine

pH Dependence

Optimum pH is 9.0.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site217S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site240S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site260S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site261S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site274S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site278Proton acceptor

GO annotations

AspectTerm
Molecular Functionisoliquiritigenin 2'-O-methyltransferase activity
Molecular Functionlicodione 2'-O-methyltransferase activity
Molecular Functionprotein dimerization activity
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Isoliquiritigenin 2'-O-methyltransferase
  • EC number
  • Short names
    MsCHMT
  • Alternative names
    • Chalcone O-methyltransferase (ChOMT)
    • Licodione 2'-O-methyltransferase (MsLMT) (EC:2.1.1.65
      ) . EC:2.1.1.65 (UniProtKB | ENZYME | Rhea)

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Apollo
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fabales > Fabaceae > Papilionoideae > 50 kb inversion clade > NPAAA clade > Hologalegina > IRL clade > Trifolieae > Medicago

Accessions

  • Primary accession
    P93324

PTM/Processing

Features

Showing features for initiator methionine, chain.

Type
IDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00002044392-372Isoliquiritigenin 2'-O-methyltransferase

Expression

Tissue specificity

Roots (at protein level). Expressed mainly in roots, and to a lesser extent in root nodules. In the roots, expression is not detected in the root tip or the cells immediately behind the tip, but is detected in tissues starting 1.5-2.0 mm distal to the root tip. Detected in the epidermal and cortical cells of 2 day old roots, with lower levels in vascular tissue.

Induction

By fungal elicitor.

Developmental stage

Levels are highest one week after planting.

Interaction

Subunit

Monomer (PubMed:1731632).
Homodimer (PubMed:11224575).

Family & Domains

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    372
  • Mass (Da)
    41,138
  • Last updated
    1997-05-01 v1
  • MD5 Checksum
    C8C6098F29B0043CD932E2B4D03D84DE
MGNSYITKEDNQISATSEQTEDSACLSAMVLTTNLVYPAVLNAAIDLNLFEIIAKATPPGAFMSPSEIASKLPASTQHSDLPNRLDRMLRLLASYSVLTSTTRTIEDGGAERVYGLSMVGKYLVPDESRGYLASFTTFLCYPALLQVWMNFKEAVVDEDIDLFKNVHGVTKYEFMGKDKKMNQIFNKSMVDVCATEMKRMLEIYTGFEGISTLVDVGGGSGRNLELIISKYPLIKGINFDLPQVIENAPPLSGIEHVGGDMFASVPQGDAMILKAVCHNWSDEKCIEFLSNCHKALSPNGKVIIVEFILPEEPNTSEESKLVSTLDNLMFITVGGRERTEKQYEKLSKLSGFSKFQVACRAFNSLGVMEFYK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
L10211
EMBL· GenBank· DDBJ
AAB48059.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our Privacy Notice.
Help