P93324 · CHOMT_MEDSA
- ProteinIsoliquiritigenin 2'-O-methyltransferase
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids372 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Methylates the 2'-hydroxyl of isoliquiritigenin and licodione. Does not methylate narigenin chalcone, caffeic acid or daidzein. Involved in the root nodulation initiation by promoting the biosynthesis of nod-inducing molecules.
Catalytic activity
- isoliquiritigenin + S-adenosyl-L-methionine = 2'-O-methylisoliquiritigenin + S-adenosyl-L-homocysteine + H+
Activity regulation
Inhibited by 1 mM Co2+, Cu2+, Zn2+ or Fe2+. Non-competitively inhibited by S-adenosyl-L-homocysteine. Competitively inhibited by 2'-O-methylisoliquiritigenin.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
2.2 μM | isoliquiritigenin | |||||
17.7 μM | S-adenosyl-L-methionine |
pH Dependence
Optimum pH is 9.0.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 217 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 240 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 260 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 261 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 274 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Active site | 278 | Proton acceptor | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | isoliquiritigenin 2'-O-methyltransferase activity | |
Molecular Function | licodione 2'-O-methyltransferase activity | |
Molecular Function | protein dimerization activity | |
Biological Process | methylation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameIsoliquiritigenin 2'-O-methyltransferase
- EC number
- Short namesMsCHMT
- Alternative names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fabales > Fabaceae > Papilionoideae > 50 kb inversion clade > NPAAA clade > Hologalegina > IRL clade > Trifolieae > Medicago
Accessions
- Primary accessionP93324
PTM/Processing
Features
Showing features for initiator methionine, chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Initiator methionine | 1 | Removed | |||
Chain | PRO_0000204439 | 2-372 | Isoliquiritigenin 2'-O-methyltransferase | ||
Expression
Tissue specificity
Roots (at protein level). Expressed mainly in roots, and to a lesser extent in root nodules. In the roots, expression is not detected in the root tip or the cells immediately behind the tip, but is detected in tissues starting 1.5-2.0 mm distal to the root tip. Detected in the epidermal and cortical cells of 2 day old roots, with lower levels in vascular tissue.
Induction
By fungal elicitor.
Developmental stage
Levels are highest one week after planting.
Structure
Family & Domains
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. COMT subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length372
- Mass (Da)41,138
- Last updated1997-05-01 v1
- MD5 ChecksumC8C6098F29B0043CD932E2B4D03D84DE
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
L10211 EMBL· GenBank· DDBJ | AAB48059.1 EMBL· GenBank· DDBJ | mRNA |