P56930 · RL25_THETH
- ProteinLarge ribosomal subunit protein bL25
- GenerplY
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids206 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
This is one of 3 proteins that mediate the attachment of the 5S rRNA onto the large ribosomal subunit.
Miscellaneous
Despite its considerably larger size this protein can substitute for the endogenous E.coli bL25 protein in vitro.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytosolic large ribosomal subunit | |
Molecular Function | 5S rRNA binding | |
Molecular Function | structural constituent of ribosome | |
Biological Process | translation |
Keywords
- Molecular function
Names & Taxonomy
Protein names
- Recommended nameLarge ribosomal subunit protein bL25
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Deinococcota > Deinococci > Thermales > Thermaceae > Thermus
Accessions
- Primary accessionP56930
- Secondary accessions
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 10 | Destabilizes formation of the 5S rRNA/N-terminal bL25 complex. | |||
Mutagenesis | 14 | No effect on 5S rRNA/N-terminal bL25 complex formation. | |||
Mutagenesis | 16 | No effect on 5S rRNA/N-terminal bL25 complex formation. | |||
Mutagenesis | 19 | Destabilizes formation of the 5S rRNA/N-terminal bL25 complex. | |||
Mutagenesis | 20 | No effect on 5S rRNA/N-terminal bL25 complex formation. | |||
Mutagenesis | 29 | No 5S rRNA/N-terminal bL25 complex formed. | |||
Mutagenesis | 85 | Destabilizes formation of the 5S rRNA/N-terminal bL25 complex. | |||
Mutagenesis | 85 | No 5S rRNA/N-terminal bL25 complex formed. | |||
Mutagenesis | 87 | Strongly destabilizes formation of the 5S rRNA/N-terminal bL25 complex. | |||
Mutagenesis | 87 | No 5S rRNA/N-terminal bL25 complex formed. | |||
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000181609 | 1-206 | Large ribosomal subunit protein bL25 | ||
Interaction
Subunit
Part of the 50S ribosomal subunit. Contacts the 5S rRNA.
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-91 | bL25 domain | |||
Region | 92-206 | CTC domain | |||
Region | 184-206 | Disordered | |||
Compositional bias | 195-206 | Basic and acidic residues | |||
Domain
The N-terminal 91 amino acids are capable of binding to 5S rRNA and also of displacing full-length protein bound to 5S rRNA. The first 80 amino acids are not sufficient.
Sequence similarities
Belongs to the bacterial ribosomal protein bL25 family. CTC subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length206
- Mass (Da)23,219
- Last updated2005-03-29 v3
- MD5 Checksum12ADD7DF7391FE76A0B77825A56EBAC2
Features
Showing features for sequence conflict, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 27 | in Ref. 2; AA sequence | |||
Compositional bias | 195-206 | Basic and acidic residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X94435 EMBL· GenBank· DDBJ | CAA64209.1 EMBL· GenBank· DDBJ | Genomic DNA |