P28241 · IDH2_YEAST
- ProteinIsocitrate dehydrogenase [NAD] subunit 2, mitochondrial
- GeneIDH2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids369 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Performs an essential role in the oxidative function of the citric acid cycle. Also binds RNA; specifically to the 5'-untranslated leaders of mitochondrial mRNAs.
Miscellaneous
Present with 43100 molecules/cell in log phase SD medium.
Catalytic activity
- D-threo-isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Binds 1 Mg2+ or Mn2+ ion per subunit.
Activity regulation
Allosterically regulated by several compounds including AMP, NAD+, and citrate.
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 119 | substrate | ||||
Sequence: R | ||||||
Binding site | 129 | substrate | ||||
Sequence: R | ||||||
Binding site | 150 | substrate | ||||
Sequence: R | ||||||
Site | 157 | Critical for catalysis | ||||
Sequence: Y | ||||||
Site | 204 | Critical for catalysis | ||||
Sequence: K | ||||||
Binding site | 237 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 237 | substrate | ||||
Sequence: D | ||||||
Binding site | 263 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 267 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | isocitrate dehydrogenase complex (NAD+) | |
Cellular Component | mitochondrial matrix | |
Cellular Component | mitochondrion | |
Molecular Function | isocitrate dehydrogenase (NAD+) activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | NAD binding | |
Molecular Function | RNA binding | |
Biological Process | glutamate biosynthetic process | |
Biological Process | isocitrate metabolic process | |
Biological Process | tricarboxylic acid cycle |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameIsocitrate dehydrogenase [NAD] subunit 2, mitochondrial
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionP28241
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for transit peptide, chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-15 | Mitochondrion | ||||
Sequence: MLRNTFFRNTSRRFL | ||||||
Chain | PRO_0000014434 | 16-369 | Isocitrate dehydrogenase [NAD] subunit 2, mitochondrial | |||
Sequence: ATVKQPSIGRYTGKPNPSTGKYTVSFIEGDGIGPEISKSVKKIFSAANVPIEWESCDVSPIFVNGLTTIPDPAVQSITKNLVALKGPLATPIGKGHRSLNLTLRKTFGLFANVRPAKSIEGFKTTYENVDLVLIRENTEGEYSGIEHIVCPGVVQSIKLITRDASERVIRYAFEYARAIGRPRVIVVHKSTIQRLADGLFVNVAKELSKEYPDLTLETELIDNSVLKVVTNPSAYTDAVSVCPNLYGDILSDLNSGLSAGSLGLTPSANIGHKISIFEAVHGSAPDIAGQDKANPTALLLSSVMMLNHMGLTNHADQIQNAVLSTIASGPENRTGDLAGTATTSSFTEAVIKRL | ||||||
Modified residue | 105 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 153 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 327 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 349 | Phosphothreonine | ||||
Sequence: T |
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Octamer of two non-identical subunits IDH1 and IDH2.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | P28241 | IDH1 P28834 | 7 | EBI-8883, EBI-8878 |
Protein-protein interaction databases
Miscellaneous
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length369
- Mass (Da)39,739
- Last updated1992-12-01 v1
- Checksum3A48C999776CE373
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 25 | in Ref. 6; AA sequence | ||||
Sequence: R → G |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M74131 EMBL· GenBank· DDBJ | AAA34702.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X94335 EMBL· GenBank· DDBJ | CAA64054.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z75043 EMBL· GenBank· DDBJ | CAA99335.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X90518 EMBL· GenBank· DDBJ | CAA62110.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006948 EMBL· GenBank· DDBJ | DAA10909.1 EMBL· GenBank· DDBJ | Genomic DNA |