P28002 · COMT1_MEDSA
- ProteinCaffeic acid 3-O-methyltransferase
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids365 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the conversion of caffeic acid to ferulic acid and of 5-hydroxyferulic acid to sinapic acid (Probable) (PubMed:1898010).
The resulting products may subsequently be converted to the corresponding alcohols that are incorporated into lignins (Probable) (PubMed:1898010).
The resulting products may subsequently be converted to the corresponding alcohols that are incorporated into lignins (Probable) (PubMed:1898010).
Catalytic activity
- (E)-caffeate + S-adenosyl-L-methionine = (E)-ferulate + S-adenosyl-L-homocysteine + H+This reaction proceeds in the forward direction.
- (E)-5-hydroxyferulate + S-adenosyl-L-methionine = (E)-sinapate + S-adenosyl-L-homocysteine + H+This reaction proceeds in the forward direction.
Pathway
Aromatic compound metabolism; phenylpropanoid biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 131 | (E)-ferulate (UniProtKB | ChEBI) | |||
Binding site | 208 | S-adenosyl-L-homocysteine (UniProtKB | ChEBI) | |||
Binding site | 231 | S-adenosyl-L-homocysteine (UniProtKB | ChEBI) | |||
Binding site | 251 | S-adenosyl-L-homocysteine (UniProtKB | ChEBI) | |||
Binding site | 252 | S-adenosyl-L-homocysteine (UniProtKB | ChEBI) | |||
Binding site | 264 | S-adenosyl-L-homocysteine (UniProtKB | ChEBI) | |||
Binding site | 265 | S-adenosyl-L-homocysteine (UniProtKB | ChEBI) | |||
Active site | 269 | Proton acceptor | |||
Binding site | 270 | (E)-5-hydroxyferulate (UniProtKB | ChEBI) | |||
Active site | 297 | ||||
Active site | 329 | ||||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | caffeate O-methyltransferase activity | |
Molecular Function | protein dimerization activity | |
Molecular Function | S-adenosylmethionine-dependent methyltransferase activity | |
Biological Process | lignin biosynthetic process | |
Biological Process | methylation | |
Biological Process | phenylpropanoid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCaffeic acid 3-O-methyltransferase
- EC number
- Short namesCAOMT ; COMT
- Alternative names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fabales > Fabaceae > Papilionoideae > 50 kb inversion clade > NPAAA clade > Hologalegina > IRL clade > Trifolieae > Medicago
Accessions
- Primary accessionP28002
- Secondary accessions
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000063204 | 1-365 | Caffeic acid 3-O-methyltransferase | ||
Expression
Tissue specificity
More abundant in roots and stems.
Induction
By infection, plant wounding, or elicitor treatment of cell cultures.
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Sequence similarities
Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family. COMT subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length365
- Mass (Da)39,946
- Last updated1992-08-01 v1
- MD5 ChecksumDCB1AAF03F93D8D0B6D174AC70078416
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 88 | in Ref. 2; ACY06328 | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M63853 EMBL· GenBank· DDBJ | AAB46623.1 EMBL· GenBank· DDBJ | mRNA | ||
GU066087 EMBL· GenBank· DDBJ | ACY06328.1 EMBL· GenBank· DDBJ | mRNA |