P21853 · PHNS_NITV9
- ProteinPeriplasmic [NiFe] hydrogenase small subunit
- GenehydA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids317 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the reversible oxidoreduction of molecular hydrogen, in conjunction with a specific electron acceptor, cytochrome c3.
Catalytic activity
- 2 Fe(III)-[cytochrome c3] + H2 = 2 Fe(II)-[cytochrome c3] + 2 H+
Cofactor
Protein has several cofactor binding sites:
Note: Binds 2 [4Fe-4S] clusters.
Note: Binds 1 [3Fe-4S] cluster.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 67 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 70 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 164 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 200 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 238 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 241 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 266 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 272 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 281 | [3Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 299 | [3Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 302 | [3Fe-4S] cluster (UniProtKB | ChEBI) | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | [Ni-Fe] hydrogenase complex | |
Cellular Component | ferredoxin hydrogenase complex | |
Cellular Component | membrane | |
Cellular Component | periplasmic space | |
Molecular Function | 3 iron, 4 sulfur cluster binding | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | cytochrome-c3 hydrogenase activity | |
Molecular Function | electron transfer activity | |
Molecular Function | ferredoxin hydrogenase activity | |
Molecular Function | metal ion binding | |
Biological Process | anaerobic respiration |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePeriplasmic [NiFe] hydrogenase small subunit
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Thermodesulfobacteriota > Desulfovibrionia > Desulfovibrionales > Desulfovibrionaceae > Nitratidesulfovibrio
Accessions
- Primary accessionP21853
- Secondary accessions
Subcellular Location
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-50 | Tat-type signal | ||||
Sequence: MKISIGLGKEGVEERLAERGVSRRDFLKFCTAIAVTMGMGPAFAPEVARA | ||||||
Chain | PRO_0000013419 | 51-317 | Periplasmic [NiFe] hydrogenase small subunit | |||
Sequence: LMGPRRPSVVYLHNAECTGCSESVLRAFEPYIDTLILDTLSLDYHETIMAAAGDAAEAALEQAVNSPHGFIAVVEGGIPTAANGIYGKVANHTMLDICSRILPKAQAVIAYGTCATFGGVQAAKPNPTGAKGVNDALKHLGVKAINIAGCPPNPYNLVGTIVYYLKNKAAPELDSLNRPTMFFGQTVHEQCPRLPHFDAGEFAPSFESEEARKGWCLYELGCKGPVTMNNCPKIKFNQTNWPVDAGHPCIGCSEPDFWDAMTPFYQN |
Post-translational modification
Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length317
- Mass (Da)34,113
- Last updated1991-05-01 v1
- Checksum2EAF2D471932C8A8
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M58339 EMBL· GenBank· DDBJ | AAA23369.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP001197 EMBL· GenBank· DDBJ | ACL07228.1 EMBL· GenBank· DDBJ | Genomic DNA |