P20047 · CADC_STAAU
- ProteinCadmium resistance transcriptional regulatory protein CadC
- GenecadC
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids122 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Metal-binding repressor for the cad operon. Involved in resistance to heavy metals, such as cadmium, bismuth, zinc or lead. Binds 2 metal ions per subunit. Metal binding to the N-terminal regulatory site causes the repressor to dissociate from the DNA.
Miscellaneous
The N-terminal, regulatory metal binding site 1 has higher affinity for cadmium than for zinc. The second metal binding site has higher affinity for zinc and has no regulatory function.
Features
Showing features for binding site, dna binding.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 7 | Cd2+ (UniProtKB | ChEBI); ligand shared between dimeric partners | |||
Binding site | 11 | Cd2+ (UniProtKB | ChEBI); ligand shared between dimeric partners | |||
Binding site | 58 | Cd2+ (UniProtKB | ChEBI); ligand shared between dimeric partners | |||
DNA binding | 59-78 | H-T-H motif | |||
Binding site | 60 | Cd2+ (UniProtKB | ChEBI); ligand shared between dimeric partners | |||
Binding site | 101 | Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners | |||
Binding site | 103 | Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners | |||
Binding site | 114 | Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners | |||
Binding site | 117 | Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | DNA binding | |
Molecular Function | DNA-binding transcription factor activity | |
Molecular Function | metal ion binding | |
Biological Process | response to cadmium ion |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameCadmium resistance transcriptional regulatory protein CadC
- Alternative names
Gene names
Encoded on
- Plasmid pI258
Organism names
- Organism
- Taxonomic lineageBacteria > Bacillati > Bacillota > Bacilli > Bacillales > Staphylococcaceae > Staphylococcus
Accessions
- Primary accessionP20047
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 7 | Abolishes dissociation from DNA; when associated with G-11; S-58 and S-60. | |||
Mutagenesis | 11 | No effect. Abolishes dissociation from DNA; when associated with S-7; S-58 and S-60. | |||
Mutagenesis | 58 | Abolishes dissociation from DNA; when associated with S-7; G-11 and S-60. | |||
Mutagenesis | 60 | Abolishes dissociation from DNA; when associated with S-7; G-11 and S-58. | |||
Mutagenesis | 101 | No effect on repressor activity; when associated with A-103. | |||
Mutagenesis | 103 | No effect on repressor activity; when associated with G-101. | |||
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000160620 | 1-122 | Cadmium resistance transcriptional regulatory protein CadC | ||
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length122
- Mass (Da)13,779
- Last updated1991-02-01 v1
- MD5 ChecksumED3942C77A4814591F1EACF5D3C36866
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
J04551 EMBL· GenBank· DDBJ | AAB59153.1 EMBL· GenBank· DDBJ | Genomic DNA |