P11828 · GLYG3_SOYBN

Function

function

Glycinin is the major seed storage protein of soybean (PubMed:2485233).
Glycinin basic peptides (GBPs), and, to a lower extent, glycinin exhibit antibacterial activity against Gram-negative and Gram-positive bacteria (e.g. L.monocytogenes, B.subtilis, E.coli and S.enteritidis) by forming pores and aggregating in transmembranes, leading to membrane permeability and, eventually, cell death (PubMed:22236762, PubMed:28590128, Ref.12).

Biotechnology

Emulsification efficiency of glycinin is improved by degree-dependent glycation with soy soluble polysaccharide (SSPS) at 60 degrees Celsius in both the acidic (A) and basic (B) polypeptides as a result of subunit dissociation at the quaternary level (PubMed:30372068).
Thermal treatment of soybean seed proteins leads to the aggregation of glycinin acidic and basic polypeptides (GAP and GBP, respectively) (PubMed:10867183, PubMed:25801436).
GBP improve sensory properties of meat (e.g. pork) during chilled storage and inhibit bacterial growth (e.g. L.monocytogenes, B.subtilis, E.coli and S.enteritidis) (PubMed:22236762, PubMed:30263339).
Antibacterial properties of the GBP antimicrobial peptides (AMPs) associated with no cytotoxicity on the viability of human embryonic kidney cells make them promising candidates as natural antibacterial agents (PubMed:22236762, PubMed:28590128, Ref.12). Fragmented peptides resulting from gastrointestinal digestion of germinated soybeans seem to have anticancer and anti-inflammatory actions on human colon cancer cells (e.g. Caco-2, HT-29, and HCT-116) and macrophages (LPS-stimulated RAW 264.7) (PubMed:29037738).
Such peptides resulting from digested germinated soybeans exhibit also anti-diabetic potential by inhibiting dipeptidyl peptidase IV (DPP-IV), salivary alpha-amylase and intestinal alpha-glucosidase enzymes (PubMed:30249015).

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum
Cellular Componentprotein storage vacuole
Molecular Functionnutrient reservoir activity

Keywords

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Glycinin G3
  • Short names
    Glycinin 11S G3
    ; Glycinin A1bB2
  • Cleaved into 2 chains
  • Allergen name
    Gly m 6

Gene names

    • Name
      GY3
    • Ordered locus names
      Glyma19g34780

Organism names

  • Taxonomic identifier
  • Strains
    • cv. Dare
    • cv. Matsuura
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Fabales > Fabaceae > Papilionoideae > 50 kb inversion clade > NPAAA clade > indigoferoid/millettioid clade > Phaseoleae > Glycine > Glycine subgen. Soja

Accessions

  • Primary accession
    P11828
  • Secondary accessions
    • Q852U4
    • Q852U5

Proteomes

Genome annotation databases

Subcellular Location

Endoplasmic reticulum
Note: Hexamers are assembled in the endoplasmic reticulum and later sorted to the protein storage vacuoles.

Keywords

Phenotypes & Variants

Allergenic properties

Causes an allergic reaction in human and animals (e.g. rats, mouse and piglets); the acidic subunit is particularly allergenic (PubMed:18996574, PubMed:23426933, PubMed:24499064).
Binds to IgE of patients with severe allergic reactions (anaphylaxis) to soybean (PubMed:18996574).
Allergy to soybean is most common for infants (usually appears at the age of three months) which frequently outgrow their soybean allergy by the age of two, but a severe soybean allergy can last a lifetime; various symptoms involve skin, gastrointestinal tract and respiratory tracts (PubMed:24499064).
Damaged intestinal function in piglets is associated with glycinin-mediated perturbation of nuclear factor-kappa B (NF-kappaB), Jun N-terminal kinase (JNK) and p38 levels (PubMed:30139257).
Juvenile Chinese mitten crabs (E.sinensis) supplemented with glycinin display impaired growth and altered intestinal health due to gut inflammation, reshaped community of gut microbiota and digestive dysfunction (PubMed:30300740).
Ingredient processing methods to reduce soybean allergenicity but keeping its nutritional values have been developed, among them physical processing includes extrusion, high-pressure (>300 MPa), heating (between 70 and 90 degrees Celsius), roasting, chemical processing includes ethanol extraction (55-76 percent between 70 and 80 degrees Celsius), in vitro glycation (e.g. with xylose at 55 degrees Celsius) and enzymatic hydrolysis with pepsin and trypsin, and biological processing includes fermentation with A.oryzae, S.cerevisiae, L.lactic subsplactis, B.subtilis, B.lactic and L.plantarum (PubMed:24499064, PubMed:27620509).
Resistant to hydrolysis by papain, alcalase, and fungal protease (PubMed:24499064).

Keywords

Protein family/group databases

PTM/Processing

Features

Showing features for signal, chain, disulfide bond, propeptide.

TypeIDPosition(s)Description
Signal1-21
ChainPRO_000003201722-296Glycinin A1b subunit
Disulfide bond31↔64
Disulfide bond107↔303Interchain (between A and B chains)
ChainPRO_0000032018297-476Glycinin B2 subunit
PropeptidePRO_0000032019477-481

Post-translational modification

During soybean germination, seed storage proteins are hydrolyzed by protease/26S proteasome.

Keywords

Proteomic databases

Expression

Tissue specificity

Confined to developing seeds.

Developmental stage

Progressive level increase from pod to full-size seed growth.

Interaction

Subunit

Hexamer; each subunit is composed of an acidic and a basic chain derived from a single precursor and linked by a disulfide bond.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, region, compositional bias, motif.

TypeIDPosition(s)Description
Domain36-240Cupin type-1 1
Region193-218Disordered
Region245-294Disordered
Compositional bias271-294Basic and acidic residues
Domain309-458Cupin type-1 2
Motif472-481Vacuolar targeting signal

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.

This entry describes 2 isoforms produced by Alternative splicing.

P11828-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    481
  • Mass (Da)
    54,242
  • Last updated
    1989-10-01 v1
  • Checksum
    5F3C3148DF6241A7
MAKLVLSLCFLLFSGCCFAFSFREQPQQNECQIQRLNALKPDNRIESEGGFIETWNPNNKPFQCAGVALSRCTLNRNALRRPSYTNAPQEIYIQQGSGIFGMIFPGCPSTFEEPQQKGQSSRPQDRHQKIYHFREGDLIAVPTGFAYWMYNNEDTPVVAVSLIDTNSFQNQLDQMPRRFYLAGNQEQEFLQYQPQKQQGGTQSQKGKRQQEEENEGGSILSGFAPEFLEHAFVVDRQIVRKLQGENEEEEKGAIVTVKGGLSVISPPTEEQQQRPEEEEKPDCDEKDKHCQSQSRNGIDETICTMRLRHNIGQTSSPDIFNPQAGSITTATSLDFPALSWLKLSAQFGSLRKNAMFVPHYNLNANSIIYALNGRALVQVVNCNGERVFDGELQEGQVLIVPQNFAVAARSQSDNFEYVSFKTNDRPSIGNLAGANSLLNALPEEVIQQTFNLRRQQARQVKNNNPFSFLVPPKESQRRVVA

P11828-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Features

Showing features for sequence conflict, alternative sequence, compositional bias.

TypeIDPosition(s)Description
Sequence conflict42in Ref. 3; BAC55938/BAC55937
Alternative sequenceVSP_06014472-75in isoform 2
Sequence conflict97in Ref. 3; BAC55938
Sequence conflict145in Ref. 3; BAC55938
Sequence conflict156in Ref. 3; BAC55938/BAC55937
Sequence conflict162in Ref. 3; BAC55938
Sequence conflict168-169in Ref. 3; BAC55938
Sequence conflict186in Ref. 3; BAC55938
Sequence conflict194in Ref. 3; BAC55938
Sequence conflict219in Ref. 3; BAC55938/BAC55937
Sequence conflict238in Ref. 3; BAC55938
Sequence conflict258in Ref. 3; BAC55938
Sequence conflict271-272in Ref. 3; BAC55938
Compositional bias271-294Basic and acidic residues
Sequence conflict306in Ref. 3; BAC55938
Sequence conflict399in Ref. 3; BAC55938

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X15123
EMBL· GenBank· DDBJ
CAA33217.1
EMBL· GenBank· DDBJ
Genomic DNA
AB030494
EMBL· GenBank· DDBJ
BAC55937.1
EMBL· GenBank· DDBJ
mRNA
AB030495
EMBL· GenBank· DDBJ
BAC55938.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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