P0AD49 · YFIA_ECOLI
- ProteinRibosome-associated inhibitor A
- GeneraiA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids113 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
During stationary phase prevents 70S dimer formation, probably in order to regulate translation efficiency during transition between the exponential and the stationary phases (PubMed:16324148).
During environmental stress such as cold shock or excessive cell density at stationary phase, stabilizes the 70S ribosome against dissociation, inhibits translation elongation and increases translation accuracy (PubMed:11375931, PubMed:15219834).
When normal growth conditions are restored, is quickly released from the ribosome (PubMed:11375931).
Has been suggested to inhibit translation elongation by blocking the A-site (aminoacyl-tRNA site) (PubMed:11375931).
Has also been suggested to inhibit translation initiation by blocking the A-site and P-site (peptidyl-tRNA site) of the ribosome (PubMed:15502846, PubMed:23420694).
At 15 degrees Celsius binds 30S subunits and stimulates their association with 50S subunits into idle 70S ribosomes (PubMed:23420694).
Crystallization with T.thermophilus 70S ribosomes shows it binds in the channel between the head and body of the 30S subunit, where mRNA, tRNAs, initiation factors IF1 and IF3 and elongation factor G would bind; this protein's extended tail follows the mRNA channel and probably prevents RMF binding, which would prevent ribosome dimerization (PubMed:22605777).
This protein also stabilizes the 30S head relative to the rest of the ribosome, which may also prevent dimerization (PubMed:22605777).
Counteracts miscoding (translation errors) particularly efficiently at magnesium concentrations close to those observed in vivo but less efficiently at higher concentrations (PubMed:15219834).
Counteraction of miscoding was shown to be stronger than inhibition of translation, suggesting that the former activity could be the main function of this protein in vivo (PubMed:15219834).
During environmental stress such as cold shock or excessive cell density at stationary phase, stabilizes the 70S ribosome against dissociation, inhibits translation elongation and increases translation accuracy (PubMed:11375931, PubMed:15219834).
When normal growth conditions are restored, is quickly released from the ribosome (PubMed:11375931).
Has been suggested to inhibit translation elongation by blocking the A-site (aminoacyl-tRNA site) (PubMed:11375931).
Has also been suggested to inhibit translation initiation by blocking the A-site and P-site (peptidyl-tRNA site) of the ribosome (PubMed:15502846, PubMed:23420694).
At 15 degrees Celsius binds 30S subunits and stimulates their association with 50S subunits into idle 70S ribosomes (PubMed:23420694).
Crystallization with T.thermophilus 70S ribosomes shows it binds in the channel between the head and body of the 30S subunit, where mRNA, tRNAs, initiation factors IF1 and IF3 and elongation factor G would bind; this protein's extended tail follows the mRNA channel and probably prevents RMF binding, which would prevent ribosome dimerization (PubMed:22605777).
This protein also stabilizes the 30S head relative to the rest of the ribosome, which may also prevent dimerization (PubMed:22605777).
Counteracts miscoding (translation errors) particularly efficiently at magnesium concentrations close to those observed in vivo but less efficiently at higher concentrations (PubMed:15219834).
Counteraction of miscoding was shown to be stronger than inhibition of translation, suggesting that the former activity could be the main function of this protein in vivo (PubMed:15219834).
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | cytosolic small ribosomal subunit | |
Molecular Function | ribosomal small subunit binding | |
Molecular Function | rRNA binding | |
Biological Process | dormancy process | |
Biological Process | negative regulation of translational elongation | |
Biological Process | negative regulation of translational initiation | |
Biological Process | response to cold |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRibosome-associated inhibitor A
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP0AD49
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
Disruption phenotype
Non-essential gene, increased formation of inactive 100S ribosomes in stationary phase, which persist longer than in wild-type. Double hpr-yfiA deletion mutants form 90S ribosomes (PubMed:16324148).
A quadruple yfiA-hpf-rmf-sra knockout strain is significantly outcompeted by wild-type after 4 days growth (PubMed:17277072).
No visible effect on viability or growth rate at 37 or 10 degrees Celsius, slight effect on bulk translation and timing of expression of some cold-shock proteins (PubMed:23420694).
A quadruple yfiA-hpf-rmf-sra knockout strain is significantly outcompeted by wild-type after 4 days growth (PubMed:17277072).
No visible effect on viability or growth rate at 37 or 10 degrees Celsius, slight effect on bulk translation and timing of expression of some cold-shock proteins (PubMed:23420694).
Interaction
Subunit
Associates mainly with 70S ribosomes (PubMed:11168583, PubMed:11375931).
Localized at the surface of the 30S ribosomal subunit, at the interface with the 50S subunit (PubMed:10535924, PubMed:15502846, PubMed:22605777).
Binds across the channel in the 30S subunit where tRNAs and mRNA interact during protein biosynthesis (PubMed:15502846, PubMed:22605777).
Can also associate with 100S ribosomes, which are inactive dimers of 70S ribosomes (PubMed:11168583).
Contacts modifed methylated residues of 16S rRNA (in complex with T.thermophilus ribosome, 2/3 residues are also modified in E.coli) (PubMed:25775268).
Localized at the surface of the 30S ribosomal subunit, at the interface with the 50S subunit (PubMed:10535924, PubMed:15502846, PubMed:22605777).
Binds across the channel in the 30S subunit where tRNAs and mRNA interact during protein biosynthesis (PubMed:15502846, PubMed:22605777).
Can also associate with 100S ribosomes, which are inactive dimers of 70S ribosomes (PubMed:11168583).
Contacts modifed methylated residues of 16S rRNA (in complex with T.thermophilus ribosome, 2/3 residues are also modified in E.coli) (PubMed:25775268).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | IntAct | |
---|---|---|---|---|---|
BINARY | P0AD49 | frr P0A805 | 4 | EBI-1129692, EBI-1114349 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 23-26 | Interaction with 16S rRNA | |||
Region | 83-91 | Interaction with 16S rRNA | |||
Region | 91-113 | Disordered | |||
Sequence similarities
Belongs to the HPF/YfiA ribosome-associated protein family. YfiA subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length113
- Mass (Da)12,785
- Last updated2007-01-23 v2
- MD5 Checksum56A38EA72F0BE578BFEF7F17B93BD275
Mass Spectrometry
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M10431 EMBL· GenBank· DDBJ | AAA24328.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z70523 EMBL· GenBank· DDBJ | CAA94436.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U00096 EMBL· GenBank· DDBJ | AAC75646.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAA16481.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
M58024 EMBL· GenBank· DDBJ | AAA62782.1 EMBL· GenBank· DDBJ | Genomic DNA |