P0A9G8 · MODE_ECOLI

  • Protein
    DNA-binding transcriptional dual regulator ModE
  • Gene
    modE
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Functions as an intracellular molybdate sensor. The ModE-Mo complex acts as a repressor of the modABC operon, which is involved in the transport of molybdate (PubMed:8550508).
Binds modA promoter DNA in the absence of molybdate, however molybdate binding confers increased DNA affinity (PubMed:9044285, PubMed:9210473).
Binds the promoter of moaA activating its transcription; binding is not enhanced by molybdate (PubMed:9044285).
The protein dimer binds the consensus palindrome sequence 5'-TATAT-N7-TAYAT-3' and a variant 5'-TGTGT-N7-TGYGT-3' (PubMed:16205910, PubMed:9044285, PubMed:9210473).
Acts as a regulator of the expression of 67 genes, many of which encode molybdoenzymes, acts both directly and indirectly (PubMed:10206709, PubMed:16205910, PubMed:9466267).
ModE also binds tungstate (PubMed:11259434, PubMed:9210473).

Activity regulation

The ModE dimer binds two molecules of molybdate (MoO42-) with a Kd of 0.8 uM, which results in major changes in the conformation of the DNA-binding domain and confers high-affinity DNA-binding to the transcription factor (PubMed:11259434, PubMed:12581638, PubMed:9044285, PubMed:9210473).
Additionally molybdate binding moves the 2 Mop domains closer together, trapping the ligand between them (PubMed:12581638).
Can also bind tungstate (PubMed:11259434, PubMed:9210473).
Molybdate is bound at the dimer interface using residues from each monomer (PubMed:11259434, PubMed:12581638).

Features

Showing features for dna binding, binding site.

Type
IDPosition(s)Description
DNA binding33-79H-T-H motif
Binding site126molybdate (UniProtKB | ChEBI)
Binding site128molybdate (UniProtKB | ChEBI)
Binding site163molybdate (UniProtKB | ChEBI)
Binding site166molybdate (UniProtKB | ChEBI)
Binding site183molybdate (UniProtKB | ChEBI)
Binding site184molybdate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular ComponentModE complex
Molecular Functioncis-regulatory region sequence-specific DNA binding
Molecular Functionmolybdenum ion binding
Biological Processmolybdate ion transport
Biological Processnegative regulation of DNA-templated transcription
Biological Processnegative regulation of DNA-templated transcription initiation
Biological Processpositive regulation of DNA-templated transcription

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA-binding transcriptional dual regulator ModE

Gene names

    • Name
      modE
    • Synonyms
      modR
    • Ordered locus names
      b0761, JW0744

Organism names

  • Taxonomic identifier
  • Strains
    • K12 / MC1000 / ATCC 39531
    • K12 / BW545
    • K12 / MG1655 / ATCC 47076
    • K12 / W3110 / ATCC 27325 / DSM 5911
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    P0A9G8
  • Secondary accessions
    • P46930

Proteomes

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Loss of repression of the modABC operon (PubMed:8564363, PubMed:8931336).
Not essential for molybdopterin cofactor synthesis (PubMed:8931336).
Increased dmsA expression under aerobic conditions and reduced expression under anaerobic conditions (PubMed:9466267).
Decreased expression of hyc and narG (PubMed:10206709).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis76Partial loss of repression by ModE.
Mutagenesis125Transcription repression by ModE even in the absence of molybdate.
Mutagenesis133Transcription repression by ModE even in the absence of molybdate.
Mutagenesis216-262Transcription repression by ModE even in the absence of molybdate.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002011251-262DNA-binding transcriptional dual regulator ModE

Proteomic databases

Expression

Induction

Constitutively expressed at low levels; probably part of the modE-modF operon (Probable) (PubMed:8564363, PubMed:8931336).
Does not seem to be autoregulated (PubMed:8931336).

Interaction

Subunit

Homodimer.

Complex viewer

View interactors in UniProtKB
View CPX-2115 in Complex Portal

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, domain.

Type
IDPosition(s)Description
Region1-121I
Domain124-191Mop 1
Region125-133Required for dimer formation and molybdate binding
Domain196-260Mop 2

Domain

Deletion of the C-terminus (from residues 122 on) results in constitutive repression of modA (PubMed:8931336).
Contains two major domains: the N-terminal domain I forms a winged helix-turn-helix motif and interacts with DNA (Probable). The C-terminal domain II is the olybdate-binding component and contains a tandem repeat of the Mop domain, each of which forms a beta-barrel (Probable) (PubMed:12581638).
The N-terminal domain plays a major role in the dimerization of the protein whereas the C-terminal domain contributes to the stability of the complex (Probable)

Sequence similarities

Belongs to the ModE family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    262
  • Mass (Da)
    28,281
  • Last updated
    2005-07-19 v1
  • MD5 Checksum
    0B06EAB3EFDE23CE54D4F3D54D8C0318
MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGISYKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDVLSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKVAITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCEVLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC

Mass Spectrometry

Molecular mass is 28,271 Da. Determined by MALDI.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U07867
EMBL· GenBank· DDBJ
AAB06892.1
EMBL· GenBank· DDBJ
Genomic DNA
U27192
EMBL· GenBank· DDBJ
AAB60175.1
EMBL· GenBank· DDBJ
Genomic DNA
U34275
EMBL· GenBank· DDBJ
AAA77051.1
EMBL· GenBank· DDBJ
Genomic DNA
U00096
EMBL· GenBank· DDBJ
AAC73848.1
EMBL· GenBank· DDBJ
Genomic DNA
AP009048
EMBL· GenBank· DDBJ
BAA35425.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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