P0A8V6 · FADR_ECOLI

Function

function

Multifunctional regulator of fatty acid metabolism (PubMed:11859088, PubMed:1569108, PubMed:19854834, PubMed:21276098, PubMed:8446033, PubMed:9388199).
Represses transcription of at least eight genes required for fatty acid transport and beta-oxidation including fadA, fadB, fadD, fadL and fadE (PubMed:9388199).
Activates transcription of at least three genes required for unsaturated fatty acid biosynthesis: fabA, fabB and iclR, the gene encoding the transcriptional regulator of the aceBAK operon encoding the glyoxylate shunt enzymes (PubMed:9388199).

Activity regulation

Binding of FadR to DNA is specifically inhibited by long chain acyl-CoA compounds, but not by long chain fatty acids (PubMed:1569108).
Long chain acyl-CoA binds directly to the protein preventing it from binding DNA, which derepresses genes for beta-oxidation and prevents activation of genes for unsaturated fatty acid biosynthesis (PubMed:19854834, PubMed:7836365).

Features

Showing features for dna binding, binding site.

123920406080100120140160180200220
Type
IDPosition(s)Description
DNA binding34-69H-T-H motif
Binding site99CoA (UniProtKB | ChEBI)
Binding site103-107CoA (UniProtKB | ChEBI)
Binding site213CoA (UniProtKB | ChEBI)
Binding site219CoA (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionDNA binding
Molecular FunctionDNA-binding transcription factor activity
Molecular Functionfatty-acyl-CoA binding
Molecular Functionprotein homodimerization activity
Biological Processfatty acid metabolic process
Biological Processnegative regulation of DNA-templated transcription
Biological Processpositive regulation of DNA-templated transcription
Biological Processpositive regulation of fatty acid biosynthetic process
Biological Processregulation of fatty acid metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Fatty acid metabolism regulator protein

Gene names

    • Name
      fadR
    • Synonyms
      oleR, thdB
    • Ordered locus names
      b1187, JW1176

Organism names

  • Taxonomic identifier
  • Strains
    • K12
    • K12 / W3110 / ATCC 27325 / DSM 5911
    • K12 / MG1655 / ATCC 47076
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    P0A8V6
  • Secondary accessions
    • P09371
    • P76827

Proteomes

Subcellular Location

Keywords

Phenotypes & Variants

Disruption phenotype

Decreased transcription of fabA, low to no change in levels of fabB (PubMed:11859088, PubMed:21276098).
Up-regulation of genes involved in beta-oxidation (fatty acid degradation, at least fadA, fadB, fadE, fadH, fadI and fadJ) (PubMed:11859088).
Increased levels of fatty acids; double fadR-fabR deletions have the same phenotype, suggesting a functional fadR gene is required for fabR function (PubMed:11859088).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis9Dominant negative to wild-type, decreased DNA-binding.
Mutagenesis35Dominant negative to wild-type, decreased DNA-binding.
Mutagenesis49Dominant negative to wild-type.
Mutagenesis65Dominant negative to wild-type, decreased DNA-binding.
Mutagenesis66Dominant negative to wild-type, decreased DNA-binding.
Mutagenesis67Dominant negative to wild-type, decreased DNA-binding.
Mutagenesis215Loss of FadR repression.
Mutagenesis216Super-repressor, non-inducible phenotype, cells cannot use long chain fatty acids as carbon source.
Mutagenesis218Reduced ability to repress.
Mutagenesis219Reduced ability to repress.
Mutagenesis219Super-repressor, non-inducible phenotype, cells cannot use long chain fatty acids as carbon source. Protein has 10-fold decreased affinity for C18:1-CoA, can still bind fabADNA and alter transcription.
Mutagenesis220Loss of FadR repression.
Mutagenesis223Super-repressor, non-inducible phenotype, cells cannot use long chain fatty acids as carbon source.
Mutagenesis227Loss of FadR repression.
Mutagenesis228Reduced ability to repress.
Mutagenesis229Loss of FadR repression.

Chemistry

PTM/Processing

Features

Showing features for initiator methionine, chain.

Type
IDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00000506262-239Fatty acid metabolism regulator protein

Proteomic databases

Expression

Induction

By growth in the presence of long chain fatty acids (C14-C18) (PubMed:7836365).

Interaction

Subunit

Homodimer (PubMed:11013219, PubMed:11279025, PubMed:11296236, PubMed:9388199).
Binding of acyl-CoA alters conformation of the dimer, separating the DNA-binding regions and disrupting DNA-binding (PubMed:11296236).

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain6-74HTH gntR-type
Region215-230Binds acyl-CoA

Domain

The N-terminus binds DNA, the C-terminus (residues 83-239) is responsible for dimerization (PubMed:9388199).
The C-terminal domain binds acyl-CoA (PubMed:11296236, PubMed:7836365).

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    239
  • Mass (Da)
    26,969
  • Last updated
    2007-01-23 v2
  • MD5 Checksum
    48835AF5C3E5C8F2B7ABA321DFBE6AFF
MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVTRTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLLSVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPIYGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHESGEIWHRMQKNLPGDLAIQGR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X08087
EMBL· GenBank· DDBJ
CAA30881.1
EMBL· GenBank· DDBJ
Genomic DNA
U00096
EMBL· GenBank· DDBJ
AAC74271.1
EMBL· GenBank· DDBJ
Genomic DNA
AP009048
EMBL· GenBank· DDBJ
BAA36042.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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