UniProtKB - P0A2G9 (CBIF_SALTY)
Protein
Cobalt-precorrin-4 C(11)-methyltransferase
Gene
cbiF
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Functioni
Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5A.1 Publication
Catalytic activityi
- Co-precorrin-4 + S-adenosyl-L-methionine = Co-precorrin-5A + H+ + S-adenosyl-L-homocysteine1 PublicationEC:2.1.1.2711 Publication
: adenosylcobalamin biosynthesis Pathwayi
This protein is involved in step 4 of the subpathway that synthesizes cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route).Proteins known to be involved in the 10 steps of the subpathway in this organism are:
- Sirohydrochlorin cobaltochelatase (cbiK)
- Cobalt-precorrin-2 C(20)-methyltransferase (cbiL)
- Probable cobalt-factor III C(17)-methyltransferase (cbiH)
- Cobalt-precorrin-4 C(11)-methyltransferase (cbiF)
- Cobalt-precorrin-5A hydrolase (cbiG)
- Cobalt-precorrin-5B C(1)-methyltransferase (cbiD)
- Cobalt-precorrin-6A reductase (cbiJ)
- Cobalt-precorrin-7 C(5)-methyltransferase (cbiE), Cobalt-precorrin-6B C(15)-methyltransferase (decarboxylating) (cbiT)
- Cobalt-precorrin-8 methylmutase (cbiC)
- Cobyrinate a,c-diamide synthase (cbiA)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route), the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.
GO - Molecular functioni
- precorrin-4 C11-methyltransferase activity Source: InterPro
GO - Biological processi
- cobalamin biosynthetic process Source: UniProtKB-UniPathway
- oxidation-reduction process Source: InterPro
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | Cobalamin biosynthesis |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-13218 SENT99287:STM2029-MONOMER |
UniPathwayi | UPA00148;UER00226 |
Names & Taxonomyi
Protein namesi | Recommended name: Cobalt-precorrin-4 C(11)-methyltransferase (EC:2.1.1.271)Alternative name(s): Cobalt-precorrin-3 methylase |
Gene namesi | Name:cbiF Ordered Locus Names:STM2029 |
Organismi | Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) |
Taxonomic identifieri | 99287 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Salmonella › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000150394 | 1 – 257 | Cobalt-precorrin-4 C(11)-methyltransferaseAdd BLAST | 257 |
Proteomic databases
PaxDbi | P0A2G9 |
PRIDEi | P0A2G9 |
Family & Domainsi
Sequence similaritiesi
Belongs to the precorrin methyltransferase family.Curated
Phylogenomic databases
eggNOGi | ENOG4105CIA Bacteria COG2875 LUCA |
HOGENOMi | HOG000290520 |
KOi | K05936 |
OMAi | WSAMGEQ |
PhylomeDBi | P0A2G9 |
Family and domain databases
CDDi | cd11641 Precorrin-4_C11-MT, 1 hit |
Gene3Di | 3.30.950.10, 1 hit 3.40.1010.10, 1 hit |
InterProi | View protein in InterPro IPR000878 4pyrrol_Mease IPR035996 4pyrrol_Methylase_sf IPR014777 4pyrrole_Mease_sub1 IPR014776 4pyrrole_Mease_sub2 IPR006362 Cbl_synth_CobM/CibF IPR003043 Uropor_MeTrfase_CS |
Pfami | View protein in Pfam PF00590 TP_methylase, 1 hit |
SUPFAMi | SSF53790 SSF53790, 1 hit |
TIGRFAMsi | TIGR01465 cobM_cbiF, 1 hit |
PROSITEi | View protein in PROSITE PS00839 SUMT_1, 1 hit PS00840 SUMT_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0A2G9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSETFDPRCV WFVGAGPGDR ELITLKGYRL LQQAQVVIYA GSLINTELLD
60 70 80 90 100
YCPAQAERYD SAELHLEQII ELMAAGVKAG KTVVRLQTGD VSLYGSVREQ
110 120 130 140 150
GEELTRRGID WQVVPGVSAF LGAAAELGVE YTVPEVSQSL IITRLEGRTP
160 170 180 190 200
VPAREQLEAF ASHQTSMAIY LSVQRIHRVA ERLIAGGYPA TTPVAVIYKA
210 220 230 240 250
TWPESQTVRG TLADISDKVR DAGIRKTALI LVGNFLGKEY HYSRLYAADF
SHEYRKA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L12006 Genomic DNA Translation: AAA27258.1 AE006468 Genomic DNA Translation: AAL20933.1 |
RefSeqi | NP_460974.1, NC_003197.2 WP_000004870.1, NC_003197.2 |
Genome annotation databases
EnsemblBacteriai | AAL20933; AAL20933; STM2029 |
GeneIDi | 1253550 |
KEGGi | stm:STM2029 |
PATRICi | fig|99287.12.peg.2151 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L12006 Genomic DNA Translation: AAA27258.1 AE006468 Genomic DNA Translation: AAL20933.1 |
RefSeqi | NP_460974.1, NC_003197.2 WP_000004870.1, NC_003197.2 |
3D structure databases
SMRi | P0A2G9 |
ModBasei | Search... |
Proteomic databases
PaxDbi | P0A2G9 |
PRIDEi | P0A2G9 |
Genome annotation databases
EnsemblBacteriai | AAL20933; AAL20933; STM2029 |
GeneIDi | 1253550 |
KEGGi | stm:STM2029 |
PATRICi | fig|99287.12.peg.2151 |
Phylogenomic databases
eggNOGi | ENOG4105CIA Bacteria COG2875 LUCA |
HOGENOMi | HOG000290520 |
KOi | K05936 |
OMAi | WSAMGEQ |
PhylomeDBi | P0A2G9 |
Enzyme and pathway databases
UniPathwayi | UPA00148;UER00226 |
BioCyci | MetaCyc:MONOMER-13218 SENT99287:STM2029-MONOMER |
Family and domain databases
CDDi | cd11641 Precorrin-4_C11-MT, 1 hit |
Gene3Di | 3.30.950.10, 1 hit 3.40.1010.10, 1 hit |
InterProi | View protein in InterPro IPR000878 4pyrrol_Mease IPR035996 4pyrrol_Methylase_sf IPR014777 4pyrrole_Mease_sub1 IPR014776 4pyrrole_Mease_sub2 IPR006362 Cbl_synth_CobM/CibF IPR003043 Uropor_MeTrfase_CS |
Pfami | View protein in Pfam PF00590 TP_methylase, 1 hit |
SUPFAMi | SSF53790 SSF53790, 1 hit |
TIGRFAMsi | TIGR01465 cobM_cbiF, 1 hit |
PROSITEi | View protein in PROSITE PS00839 SUMT_1, 1 hit PS00840 SUMT_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CBIF_SALTY | |
Accessioni | P0A2G9Primary (citable) accession number: P0A2G9 Secondary accession number(s): Q05630 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2005 |
Last sequence update: | March 15, 2005 | |
Last modified: | September 18, 2019 | |
This is version 78 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - PATHWAY comments
Index of metabolic and biosynthesis pathways