P00642 · T2E1_ECOLX
- ProteinType II restriction enzyme EcoRI
- GeneecoRIR
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids277 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
A P subtype restriction enzyme that recognizes the double-stranded sequence 5'-GAATTC-3' and cleaves after G-1.
Catalytic activity
Cofactor
Note: Binds 2 magnesium ions per subunit.
Features
Showing features for active site, binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | DNA binding | |
Molecular Function | magnesium ion binding | |
Molecular Function | type II site-specific deoxyribonuclease activity | |
Biological Process | DNA restriction-modification system |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameType II restriction enzyme EcoRI
- EC number
- Short namesR.EcoRI
- Alternative names
Gene names
Encoded on
- Plasmid pMB1
- Plasmid pMB4
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionP00642
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 144 | Only nicks double strand DNA. | ||||
Sequence: E → D | ||||||
Mutagenesis | 144 | Inactivates the enzyme. | ||||
Sequence: E → Q |
Chemistry
PTM/Processing
Features
Showing features for initiator methionine, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Chain | PRO_0000077303 | 2-277 | Type II restriction enzyme EcoRI | |||
Sequence: SNKKQSNRLTEQHKLSQGVIGIFGDYAKAHDLAVGEVSKLVKKALSNEYPQLSFRYRDSIKKTEINEALKKIDPDLGGTLFVSNSSIKPDGGIVEVKDDYGEWRVVLVAEAKHQGKDIINIRNGLLVGKRGDQDLMAAGNAIERSHKNISEIANFMLSESHFPYVLFLEGSNFLTENISITRPDGRVVNLEYNSGILNRLDRLTAANYGMPINSNLCINKFVNHKDKSIMLQAASIYTQGDGREWDSKIMFEIMFDISTTSLRVLGRDLFEQLTSK |
Structure
Sequence
- Sequence statusComplete
- Length277
- Mass (Da)31,059
- Last updated2007-01-23 v2
- Checksum83EDC82868261B4B
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
J01675 EMBL· GenBank· DDBJ | AAA26371.1 EMBL· GenBank· DDBJ | Genomic DNA |