P00642 · T2E1_ECOLX

  • Protein
    Type II restriction enzyme EcoRI
  • Gene
    ecoRIR
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

A P subtype restriction enzyme that recognizes the double-stranded sequence 5'-GAATTC-3' and cleaves after G-1.

Catalytic activity

  • Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
    EC:3.1.21.4 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )
Note: Binds 2 magnesium ions per subunit.

Features

Showing features for active site, binding site.

127720406080100120140160180200220240260
TypeIDPosition(s)Description
Active site91
Binding site91Mg2+ 1 (UniProtKB | ChEBI)
Binding site91Mg2+ 2 (UniProtKB | ChEBI)
Active site111
Binding site111Mg2+ 1 (UniProtKB | ChEBI)
Active site113

GO annotations

AspectTerm
Molecular FunctionDNA binding
Molecular Functionmagnesium ion binding
Molecular Functiontype II site-specific deoxyribonuclease activity
Biological ProcessDNA restriction-modification system

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Type II restriction enzyme EcoRI
  • EC number
  • Short names
    R.EcoRI
  • Alternative names
    • Endonuclease EcoRI
    • Type-2 restriction enzyme EcoRI

Gene names

    • Name
      ecoRIR

Encoded on

  • Plasmid pMB1
  • Plasmid pMB4

Organism names

  • Taxonomic identifier
  • Strain
    • C
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    P00642

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis144Only nicks double strand DNA.
Mutagenesis144Inactivates the enzyme.

Chemistry

PTM/Processing

Features

Showing features for initiator methionine, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00000773032-277Type II restriction enzyme EcoRI

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Chemistry

Family & Domains

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    277
  • Mass (Da)
    31,059
  • Last updated
    2007-01-23 v2
  • Checksum
    83EDC82868261B4B
MSNKKQSNRLTEQHKLSQGVIGIFGDYAKAHDLAVGEVSKLVKKALSNEYPQLSFRYRDSIKKTEINEALKKIDPDLGGTLFVSNSSIKPDGGIVEVKDDYGEWRVVLVAEAKHQGKDIINIRNGLLVGKRGDQDLMAAGNAIERSHKNISEIANFMLSESHFPYVLFLEGSNFLTENISITRPDGRVVNLEYNSGILNRLDRLTAANYGMPINSNLCINKFVNHKDKSIMLQAASIYTQGDGREWDSKIMFEIMFDISTTSLRVLGRDLFEQLTSK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
J01675
EMBL· GenBank· DDBJ
AAA26371.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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