ID EGTB_MYCTU Reviewed; 425 AA. AC O69671; L0TDB1; DT 19-OCT-2011, integrated into UniProtKB/Swiss-Prot. DT 01-AUG-1998, sequence version 1. DT 16-APR-2014, entry version 75. DE RecName: Full=Iron(II)-dependent oxidoreductase EgtB; DE EC=1.8.-.-; GN Name=egtB; OrderedLocusNames=Rv3703c; OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv). OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Corynebacterineae; Mycobacteriaceae; Mycobacterium; OC Mycobacterium tuberculosis complex. OX NCBI_TaxID=83332; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 25618 / H37Rv; RX PubMed=9634230; DOI=10.1038/31159; RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., RA Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, RA Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., RA Connor R., Davies R.M., Devlin K., Feltwell T., Gentles S., Hamlin N., RA Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., RA Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., RA Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., RA Sulston J.E., Taylor K., Whitehead S., Barrell B.G.; RT "Deciphering the biology of Mycobacterium tuberculosis from the RT complete genome sequence."; RL Nature 393:537-544(1998). CC -!- FUNCTION: Catalyzes the oxidative sulfurization of hercynine via CC the addition of oxygen and gamma-glutamyl-cysteine on hercynine CC (N-alpha,N-alpha,N-alpha-trimethyl-L-histidine) (By similarity). CC -!- CATALYTIC ACTIVITY: N(alpha),N(alpha),N(alpha)-trimethyl-L- CC histidine + gamma-glutamylcysteine + 0(2) = N-(gamma-glutamyl)- CC [N(alpha),N(alpha),N(alpha)-trimethyl-L-histidinyl]-cysteine CC sulfoxide. CC -!- COFACTOR: Iron (By similarity). CC -!- PATHWAY: Amino-acid biosynthesis; ergothioneine biosynthesis. CC -!- SIMILARITY: Belongs to the sulfatase-modifying factor family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AL123456; CCP46528.1; -; Genomic_DNA. DR PIR; H70793; H70793. DR RefSeq; NP_218220.1; NC_000962.3. DR RefSeq; YP_006517193.1; NC_018143.2. DR ProteinModelPortal; O69671; -. DR SMR; O69671; 160-425. DR STRING; 83332.Rv3703c; -. DR PRIDE; O69671; -. DR EnsemblBacteria; CCP46528; CCP46528; Rv3703c. DR GeneID; 13317315; -. DR GeneID; 885128; -. DR KEGG; mtu:Rv3703c; -. DR KEGG; mtv:RVBD_3703c; -. DR PATRIC; 18156840; VBIMycTub87468_4127. DR TubercuList; Rv3703c; -. DR HOGENOM; HOG000253478; -. DR OMA; SWATRPW; -. DR OrthoDB; EOG63RGMW; -. DR ProtClustDB; CLSK872236; -. DR BioCyc; MTBRV:RV3703C-MONOMER; -. DR UniPathway; UPA01014; -. DR GO; GO:0008198; F:ferrous iron binding; ISS:UniProtKB. DR GO; GO:0016491; F:oxidoreductase activity; ISS:UniProtKB. DR GO; GO:0052704; P:ergothioneine biosynthesis from histidine via N-alpha,N-alpha,N-alpha-trimethyl-L-histidine; ISS:UniProtKB. DR Gene3D; 3.90.1580.10; -; 1. DR InterPro; IPR016187; C-type_lectin_fold. DR InterPro; IPR024775; DinB-like. DR InterPro; IPR017806; EgtB. DR InterPro; IPR005532; FGE_dom. DR Pfam; PF12867; DinB_2; 1. DR Pfam; PF03781; FGE-sulfatase; 1. DR SUPFAM; SSF56436; SSF56436; 1. DR TIGRFAMs; TIGR03440; egtB_TIGR03440; 1. PE 3: Inferred from homology; KW Complete proteome; Iron; Oxidoreductase; Reference proteome. FT CHAIN 1 425 Iron(II)-dependent oxidoreductase EgtB. FT /FTId=PRO_0000413648. FT ACT_SITE 392 392 Proton acceptor (By similarity). SQ SEQUENCE 425 AA; 47139 MW; D8CB6CB0C41E39CD CRC64; MTSPEQLACH LARARARTLR LVDFDDAELC CQYDPLMSPL VWDLAHIGQQ EELWLLRGGD PGQPGLLPPA VEGLYDAFEH SRASRVELPL LSPARARSYC ATVRSAALDA LAALPEDGDS FVFAMVISHE NQHDETMLQA LNLRTGSPLL AATSALPAGR PRMAGTSVLV AGGPFVLGVD AADEPCSLDN ERPAHVVDVP AFRIGRVPVT NGEWQDFIDD GGYTQSRWWS ERGWQHRQRA GLTAPQFWRS GGRTRTRFGH VEDIPADEPV QHVSYFEAEA YAAWAGARLP TEVEWEKACA WDPATGSRRR YPWGTEEPTD TYANLGGQTL RPAPVGAYPA GASACGAEQM LGDVWEWTTS PLRPWPGFVP MVYERYSQPF FGGDYRVLRG GSWAVEPAIL RPSFRNWDHP YRRQIFAGVR LAWDI //