N1P8M1 · N1P8M1_YEASC

Function

function

Catalyzes the first intracellular reaction of sulfate assimilation, forming adenosine-5'-phosphosulfate (APS) from inorganic sulfate and ATP. Plays an important role in sulfate activation as a component of the biosynthesis pathway of sulfur-containing amino acids.

Catalytic activity

Pathway

Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.

Features

Showing features for binding site, active site, site.

151150100150200250300350400450500
TypeIDPosition(s)Description
Binding site195sulfate (UniProtKB | ChEBI)
Binding site195-198ATP (UniProtKB | ChEBI)
Active site196
Active site197
Binding site197sulfate (UniProtKB | ChEBI)
Active site198
Site201Transition state stabilizer
Site204Transition state stabilizer
Binding site289-292ATP (UniProtKB | ChEBI)
Binding site293sulfate (UniProtKB | ChEBI)
Site328Induces change in substrate recognition on ATP binding
Binding site331ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionsulfate adenylyltransferase (ATP) activity
Biological Processcysteine biosynthetic process
Biological Processhydrogen sulfide biosynthetic process
Biological Processmethionine biosynthetic process
Biological Processsulfate assimilation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Sulfate adenylyltransferase
  • EC number
  • Alternative names
    • ATP-sulfurylase
    • Methionine-requiring protein 3
    • Sulfate adenylate transferase
      (SAT
      )

Gene names

    • Name
      MET3
    • ORF names
      CENPK1137D_1305

Organism names

Accessions

  • Primary accession
    N1P8M1

Proteomes

Subcellular Location

Keywords

PTM/Processing

Keywords

Interaction

Subunit

Homohexamer. Dimer of trimers.

Structure

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-167N-terminal
Domain3-162ATP-sulfurylase PUA-like
Region168-393Catalytic
Domain172-386Sulphate adenylyltransferase catalytic
Region394-511Required for oligomerization; adenylyl-sulfate kinase-like

Domain

The oligomerization domain is distantly related to APS kinases, but it is not functional and does not bind APS. It is required for oligomerization of the enzyme, although the oligomerization state has no effect on the catalytic activity of the enzyme.

Sequence similarities

Belongs to the sulfate adenylyltransferase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    511
  • Mass (Da)
    57,725
  • Last updated
    2013-06-26 v1
  • Checksum
    A1E7B994A9C24DFD
MPAPHGGILQDLIARDALKKNELLSEAQSSDILVWNLTPRQLCDIELILNGGFSPLTGFLNENDYSSVVTDSRLADGTLWTIPITLDVDEAFANQIKPDTRIALFQDDEIPIAILTVQDVYKPNKTIEAEKVFRGDPEHPAISYLFNVAGDYYVGGSLEAIQLPQHYDYPGLRKTPAQLRLEFQSRQWDRVVAFQTRNPMHRAHRELTVRAAREANAKVLIHPVVGLTKPGDIDHHTRVRVYQEIIKRYPNGIAFLSLLPLAMRMSGDREAVWHAIIRKNYGASHFIVGRDHAGPGKNSKGVDFYGPYDAQELVESYKHELDIEVVPFRMVTYLPDEDRYAPIDQIDTTKTRTLNISGTELRRRLRVGGEIPEWFSYPEVVKILRESNPPRPKQGFSIVLGNSLTVSREQLSIALLSTFLQFGGGRYYKIFEHNNKTELLSLIQDFIGSGSGLIIPNQWEDDKDSVVGKQNVYLLDTSSSADIQLESADEPISHIVQKVVLFLEDNGFFVF

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CM001531
EMBL· GenBank· DDBJ
EIW09535.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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