UniProtKB - I6XEI5 (LYS25_MYCTU)
Protein
Putative peptidoglycan hydrolase Rv2525c
Gene
Rv2525c
Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Functioni
May function as a peptidoglycan hydrolase with glycosidase activity (PubMed:25260828). In vitro, displays esterase activity toward p-nitrophenyl esters of various acyl chain length (C4 to C16), with a preference for p-nitrophenyl butyrate (C4) (PubMed:25869294).1 Publication1 Publication
Catalytic activityi
- Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.1 Publication EC:3.2.1.17
pH dependencei
Optimum pH is 8.0 for the hydrolysis of p-nitrophenyl butyrate.1 Publication
Temperature dependencei
Optimum temperature is 38 degrees Celsius for the hydrolysis of p-nitrophenyl butyrate.1 Publication
: peptidoglycan degradation Pathwayi
This protein is involved in the pathway peptidoglycan degradation, which is part of Cell wall degradation.1 PublicationView all proteins of this organism that are known to be involved in the pathway peptidoglycan degradation and in Cell wall degradation.
GO - Molecular functioni
- lysozyme activity Source: UniProtKB-EC
GO - Biological processi
- cell wall macromolecule catabolic process Source: UniProtKB-UniPathway
- cell wall organization Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Cell wall biogenesis/degradation |
Enzyme and pathway databases
BioCyci | MTBH37RV:G185E-6763-MONOMER |
UniPathwayi | UPA00549 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Ordered Locus Names:Rv2525cImported, LH57_13835Imported |
Organismi | Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) |
Taxonomic identifieri | 83332 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › |
Proteomesi |
|
Organism-specific databases
TubercuListi | Rv2525c |
Subcellular locationi
- Secreted 1 Publication
GO - Cellular componenti
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Disruption phenotypei
Cells lacking this gene display an increase in susceptibility to some beta-lactam antibiotics and, despite slower growth in vitro, enhanced virulence in both cellular and murine models of tuberculosis. No detectable difference in the lipid profiles.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 113 | G → A: Complete loss of esterase activity. 1 Publication | 1 | |
Mutagenesisi | 115 | S → A: 83% loss of esterase activity. 1 Publication | 1 | |
Mutagenesisi | 117 | G → A: 83% loss of esterase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 33 | Tat-type signalPROSITE-ProRule annotationAdd BLAST | 33 | |
ChainiPRO_5003706761 | 34 – 240 | Putative peptidoglycan hydrolase Rv2525cAdd BLAST | 207 |
Post-translational modificationi
Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.PROSITE-ProRule annotation1 Publication
Proteomic databases
PaxDbi | I6XEI5 |
PRIDEi | I6XEI5 |
Expressioni
Inductioni
Upon exposure to antituberculous drugs such as isoniazid, ethionamide or PA-824, Rv2525c expression is significantly up-regulated together with those of other genes involved in cell wall processes.1 Publication
Family & Domainsi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG4108WKN Bacteria ENOG4111JUS LUCA |
OMAi | HQFEIDK |
Family and domain databases
InterProi | View protein in InterPro IPR015020 DUF1906 IPR017853 Glycoside_hydrolase_SF IPR006311 TAT_signal IPR019546 TAT_signal_bac_arc |
Pfami | View protein in Pfam PF08924 DUF1906, 1 hit |
SUPFAMi | SSF51445 SSF51445, 1 hit |
TIGRFAMsi | TIGR01409 TAT_signal_seq, 1 hit |
PROSITEi | View protein in PROSITE PS51318 TAT, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
I6XEI5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSVSRRDVLK FAAATPGVLG LGVVASSLRA APASAGSLGT LLDYAAGVIP
60 70 80 90 100
ASQIRAAGAV GAIRYVSDRR PGGAWMLGKP IQLSEARDLS GNGLKIVSCY
110 120 130 140 150
QYGKGSTADW LGGASAGVQH ARRGSELHAA AGGPTSAPIY ASIDDNPSYE
160 170 180 190 200
QYKNQIVPYL RSWESVIGHQ RTGVYANSKT IDWAVNDGLG SYFWQHNWGS
210 220 230 240
PKGYTHPAAH LHQVEIDKRK VGGVGVDVNQ ILKPQFGQWA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45319.1 CP009480 Genomic DNA Translation: AIR15295.1 |
RefSeqi | NP_217041.1, NC_000962.3 WP_003412957.1, NZ_NVQJ01000032.1 |
Genome annotation databases
EnsemblBacteriai | AIR15295; AIR15295; LH57_13835 CCP45319; CCP45319; Rv2525c |
GeneIDi | 888612 |
KEGGi | mtu:Rv2525c mtv:RVBD_2525c |
PATRICi | fig|83332.111.peg.2823 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL123456 Genomic DNA Translation: CCP45319.1 CP009480 Genomic DNA Translation: AIR15295.1 |
RefSeqi | NP_217041.1, NC_000962.3 WP_003412957.1, NZ_NVQJ01000032.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4PMN | X-ray | 1.44 | A/B | 36-240 | [»] | |
4PMO | X-ray | 1.33 | A/B | 36-240 | [»] | |
4PMQ | X-ray | 1.61 | A | 36-240 | [»] | |
4PMR | X-ray | 1.81 | A | 36-240 | [»] | |
SMRi | I6XEI5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 83332.Rv2525c |
Proteomic databases
PaxDbi | I6XEI5 |
PRIDEi | I6XEI5 |
Genome annotation databases
EnsemblBacteriai | AIR15295; AIR15295; LH57_13835 CCP45319; CCP45319; Rv2525c |
GeneIDi | 888612 |
KEGGi | mtu:Rv2525c mtv:RVBD_2525c |
PATRICi | fig|83332.111.peg.2823 |
Organism-specific databases
TubercuListi | Rv2525c |
Phylogenomic databases
eggNOGi | ENOG4108WKN Bacteria ENOG4111JUS LUCA |
OMAi | HQFEIDK |
Enzyme and pathway databases
UniPathwayi | UPA00549 |
BioCyci | MTBH37RV:G185E-6763-MONOMER |
Family and domain databases
InterProi | View protein in InterPro IPR015020 DUF1906 IPR017853 Glycoside_hydrolase_SF IPR006311 TAT_signal IPR019546 TAT_signal_bac_arc |
Pfami | View protein in Pfam PF08924 DUF1906, 1 hit |
SUPFAMi | SSF51445 SSF51445, 1 hit |
TIGRFAMsi | TIGR01409 TAT_signal_seq, 1 hit |
PROSITEi | View protein in PROSITE PS51318 TAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LYS25_MYCTU | |
Accessioni | I6XEI5Primary (citable) accession number: I6XEI5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 9, 2015 |
Last sequence update: | October 3, 2012 | |
Last modified: | October 16, 2019 | |
This is version 50 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references