G8H5N1 · TS14B_SOLHA
- ProteinSesquiterpene synthase 14b
- GeneTPS14b
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids554 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Sesquiterpene synthase involved in the biosynthesis of volatile compounds (PubMed:21818683).
Mediates the conversion of (2E,6E)-farnesyl diphosphate ((EE)-FPP) into +-thujopsene, beta-bisabolene, alpha-cederene, beta-acoradiene, (E)-gamma-bisabolene, (Z)-alpha-bisabolene, (Z)-beta-farnesene and (E)-beta-farnesene, and of (2Z,6Z)-farnesyl diphosphate ((ZZ)-FPP) into (E)-gamma-bisabolene, (E)-alpha-bisabolene, (E)-beta-farnesene, (Z)-beta-farnesene, beta-bisabolene, beta-acoradiene and alpha-acoradiene (PubMed:21818683).
Can act with a low efficiency as a monoterpene synthase with geranyl diphosphate (GPP) as substrate, thus producing beta-myrcene, limonene and terpinolene (PubMed:21818683).
Mediates the conversion of (2E,6E)-farnesyl diphosphate ((EE)-FPP) into +-thujopsene, beta-bisabolene, alpha-cederene, beta-acoradiene, (E)-gamma-bisabolene, (Z)-alpha-bisabolene, (Z)-beta-farnesene and (E)-beta-farnesene, and of (2Z,6Z)-farnesyl diphosphate ((ZZ)-FPP) into (E)-gamma-bisabolene, (E)-alpha-bisabolene, (E)-beta-farnesene, (Z)-beta-farnesene, beta-bisabolene, beta-acoradiene and alpha-acoradiene (PubMed:21818683).
Can act with a low efficiency as a monoterpene synthase with geranyl diphosphate (GPP) as substrate, thus producing beta-myrcene, limonene and terpinolene (PubMed:21818683).
Catalytic activity
- (2E,6E)-farnesyl diphosphate = (E)-gamma-bisabolene + diphosphateThis reaction proceeds in the forward direction.
- (2Z,6Z)-farnesyl diphosphate = (E)-gamma-bisabolene + diphosphateThis reaction proceeds in the forward direction.
- (2Z,6Z)-farnesyl diphosphate = (E)-alpha-bisabolene + diphosphateThis reaction proceeds in the forward direction.
- (2Z,6Z)-farnesyl diphosphate = (Z)-beta-farnesene + diphosphateThis reaction proceeds in the forward direction.
- (2Z,6Z)-farnesyl diphosphate = (E)-beta-farnesene + diphosphateThis reaction proceeds in the forward direction.
- (2E,6E)-farnesyl diphosphate = (Z)-beta-farnesene + diphosphateThis reaction proceeds in the forward direction.
- (2Z,6Z)-farnesyl diphosphate = beta-acoradiene + diphosphateThis reaction proceeds in the forward direction.
- (2Z,6Z)-farnesyl diphosphate = alpha-acoradiene + diphosphateThis reaction proceeds in the forward direction.
- (2Z,6Z)-farnesyl diphosphate = beta-bisabolene + diphosphateThis reaction proceeds in the forward direction.
- (2E,6E)-farnesyl diphosphate = --alpha-cedrene + diphosphateThis reaction proceeds in the forward direction.
- (2E,6E)-farnesyl diphosphate = beta-bisabolene + diphosphateThis reaction proceeds in the forward direction.
- (2E,6E)-farnesyl diphosphate = beta-acoradiene + diphosphateThis reaction proceeds in the forward direction.
- (2Z,6Z)-farnesyl diphosphate = --alpha-cedrene + diphosphateThis reaction proceeds in the forward direction.
- (2E)-geranyl diphosphate = limonene + diphosphateThis reaction proceeds in the forward direction.
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Binds 3 Mg2+ or Mn2+ ions per subunit.
Pathway
Secondary metabolite biosynthesis; terpenoid biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 305 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 305 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 309 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 309 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 449 | Mg2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 457 | Mg2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | hydrolase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | terpene synthase activity | |
Biological Process | diterpenoid biosynthetic process | |
Biological Process | terpenoid biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Community annotation
Sesquiterpene synthase which can use either (Z,Z)-FPP or (E,E)-FPP as a substrate. In the presence of (Z,Z)-FPP the enzyme produces predominantly (Z)-beta-farnesene, alpha- and beta-acoradiene and alpha- and gamma-bisabolenes, while when assayed with (E,E)-FPP alpha-cederene, (Z)-thujopsene, beta-farnesene, beta-acoradiene and beta-bisabolene are produced.
Source | Submission date | Contributor |
---|---|---|
PubMed:21818683 | 0000-0001-5683-1696 |
Names & Taxonomy
Protein names
- Recommended nameSesquiterpene synthase 14b
- Short namesShTPS14b
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Solanales > Solanaceae > Solanoideae > Solaneae > Solanum > Solanum subgen. Lycopersicon
Accessions
- Primary accessionG8H5N1
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000454685 | 1-554 | Sesquiterpene synthase 14b | |||
Sequence: MNQLAMVNTTITRPLANYHSSVWGNYFLSYTPQLTEISSQEKRELEELKEKVRQMLVETPDNSTQKLVLIDTIQRLGVAYHFENHIKISIQNIFDEFEKNKNKDNDDDLCVVALRFRLVRGQRHYMSSDVFTRFTNDDGKFKETLTKDVQGLLNLYEATHLRVHGEEILEEALSFTVTHLKSMSPKLDNSLKAQVSEALFQPIHTNIPRVVARKYIRIYENIESHDDLLLKFAKLDFHILQKMHQRELSELTRWWKDLDHSNKYPYARDKLVECYFWAIGVYFGPQYKRARRTLTKLIVIITITDDLYDAYATYDELVPYTNAVERCEISAMHSISPYMRPLYQVFLDYFDEMEEELTKDGKAHYVYYAKIETNKWIKSYLKEAEWLKNDIIPKCEEYKRNATITISNQMNLITCLIVAGEFISKETFEWMINESLIAPASSLINRLKDDIIGHEHEQQREHGASFIECYVKEYRASKQEAYVEARRQITNAWKDINTDYLHATQVPTFVLEPALNLSRLVDILQEDDFTDSQNFLKDTITLLFVDSVNSTSCG |
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Motif | 305-309 | DDXXD motif | ||||
Sequence: DDLYD |
Domain
The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.
Sequence similarities
Belongs to the terpene synthase family. Tpsa subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length554
- Mass (Da)65,036
- Last updated2012-01-25 v1
- Checksum863D6F4D56FD8FE0