E2AZA9 · E2AZA9_CAMFO

Function

function

Core component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex 1 mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is 2 required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage. Within the MRN complex, MRE11 possesses both single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. MRE11 first endonucleolytically cleaves the 5' strand at DNA DSB ends to prevent non-homologous end joining (NHEJ) and licence HR. It then generates a single-stranded DNA gap via 3' to 5' exonucleolytic degradation, which is required for single-strand invasion and recombination.

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for active site.

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TypeIDPosition(s)Description
Active site133Proton donor

GO annotations

AspectTerm
Cellular ComponentMre11 complex
Cellular Componentsite of double-strand break
Molecular Function3'-5'-DNA exonuclease activity
Molecular Functionmanganese ion binding
Molecular Functionsingle-stranded DNA endodeoxyribonuclease activity
Biological Processdouble-strand break repair via homologous recombination
Biological Processdouble-strand break repair via nonhomologous end joining
Biological Processmeiotic DNA double-strand break formation
Biological Processmitochondrial double-strand break repair via homologous recombination
Biological Processmitotic G2 DNA damage checkpoint signaling
Biological Processmitotic intra-S DNA damage checkpoint signaling
Biological Processtelomere maintenance

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Double-strand break repair protein

Gene names

    • ORF names
      EAG_04433

Organism names

  • Taxonomic identifier
  • Strain
    • C129
  • Taxonomic lineage
    Eukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Hymenoptera > Apocrita > Aculeata > Formicoidea > Formicidae > Formicinae > Camponotus

Accessions

  • Primary accession
    E2AZA9

Proteomes

Subcellular Location

Keywords

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain295-469Mre11 DNA-binding
Region522-619Disordered
Compositional bias523-545Polar residues
Compositional bias546-561Basic and acidic residues
Compositional bias585-607Polar residues

Sequence similarities

Belongs to the MRE11/RAD32 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    619
  • Mass (Da)
    70,743
  • Last updated
    2010-11-30 v1
  • Checksum
    34A50ED45D56D5E8
MSESSNEHLDPEDTINILVATDIHLGFDYSKKRGGQSDDSFITFEEILKYGKEKEVDFILLGGDLFHDTKPTQTAMLKCVELLRKYCLGSRECKLEFLSDSELVFRHCAQKHVNYEDQNLNVSMPVFTIHGNHDDPSFGTVGSMDVLSATGLVNYFGKWTDLTRVIISPIVLKKRNTHVALYGLSYINDQRLSRLYRDEKVELLRPKDIEPFNIFVLHQNRVKHSNYAYISENRLHKFLNLVIWGHEHECRINPELNVEGGYYISQPGSSIATSLCEGESKPKHVGLLKINKNNFKMKPLKLKSVRPYIFENLILSDHDIKIHDCISLADSVSQYVDRYIENELMPKVAKQLTGYPNQPSQPLIRLRIFYNDDNEQFDTLSLAQKYCDEVANPMEMIIFRKMKSGEKSKRFNDEIDDLDDITELFQADIGQDWISTVPGGIKKYFDMEENKDKLTVLTVTALNEALNRYVDRGDIEAFRNIVSDQMKRTIEYVKAQNSEKPEDIRNEIINFRNKRVSEEQEEQSNVLRALNNPVQTNAKNVKQSHNVVSDSDDDDLNSRSSTTKTRGRGRGSRGGRGSRGSRGGAKANEGNSSITRMLSSRSAQPKRTTCDPIIIDDSD

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias523-545Polar residues
Compositional bias546-561Basic and acidic residues
Compositional bias585-607Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
GL444166
EMBL· GenBank· DDBJ
EFN61231.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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