E2AZA9 · E2AZA9_CAMFO
- ProteinDouble-strand break repair protein
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids619 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Core component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex 1 mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is 2 required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage. Within the MRN complex, MRE11 possesses both single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. MRE11 first endonucleolytically cleaves the 5' strand at DNA DSB ends to prevent non-homologous end joining (NHEJ) and licence HR. It then generates a single-stranded DNA gap via 3' to 5' exonucleolytic degradation, which is required for single-strand invasion and recombination.
Cofactor
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 133 | Proton donor | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | Mre11 complex | |
Cellular Component | site of double-strand break | |
Molecular Function | 3'-5'-DNA exonuclease activity | |
Molecular Function | manganese ion binding | |
Molecular Function | single-stranded DNA endodeoxyribonuclease activity | |
Biological Process | double-strand break repair via homologous recombination | |
Biological Process | double-strand break repair via nonhomologous end joining | |
Biological Process | meiotic DNA double-strand break formation | |
Biological Process | mitochondrial double-strand break repair via homologous recombination | |
Biological Process | mitotic G2 DNA damage checkpoint signaling | |
Biological Process | mitotic intra-S DNA damage checkpoint signaling | |
Biological Process | telomere maintenance |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameDouble-strand break repair protein
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Hymenoptera > Apocrita > Aculeata > Formicoidea > Formicidae > Formicinae > Camponotus
Accessions
- Primary accessionE2AZA9
Proteomes
Subcellular Location
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 295-469 | Mre11 DNA-binding | ||||
Sequence: FKMKPLKLKSVRPYIFENLILSDHDIKIHDCISLADSVSQYVDRYIENELMPKVAKQLTGYPNQPSQPLIRLRIFYNDDNEQFDTLSLAQKYCDEVANPMEMIIFRKMKSGEKSKRFNDEIDDLDDITELFQADIGQDWISTVPGGIKKYFDMEENKDKLTVLTVTALNEALNRY | ||||||
Region | 522-619 | Disordered | ||||
Sequence: EQSNVLRALNNPVQTNAKNVKQSHNVVSDSDDDDLNSRSSTTKTRGRGRGSRGGRGSRGSRGGAKANEGNSSITRMLSSRSAQPKRTTCDPIIIDDSD | ||||||
Compositional bias | 523-545 | Polar residues | ||||
Sequence: QSNVLRALNNPVQTNAKNVKQSH | ||||||
Compositional bias | 546-561 | Basic and acidic residues | ||||
Sequence: NVVSDSDDDDLNSRSS | ||||||
Compositional bias | 585-607 | Polar residues | ||||
Sequence: AKANEGNSSITRMLSSRSAQPKR |
Sequence similarities
Belongs to the MRE11/RAD32 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length619
- Mass (Da)70,743
- Last updated2010-11-30 v1
- Checksum34A50ED45D56D5E8
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 523-545 | Polar residues | ||||
Sequence: QSNVLRALNNPVQTNAKNVKQSH | ||||||
Compositional bias | 546-561 | Basic and acidic residues | ||||
Sequence: NVVSDSDDDDLNSRSS | ||||||
Compositional bias | 585-607 | Polar residues | ||||
Sequence: AKANEGNSSITRMLSSRSAQPKR |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
GL444166 EMBL· GenBank· DDBJ | EFN61231.1 EMBL· GenBank· DDBJ | Genomic DNA |