D9QEP1 · D9QEP1_CORP2
- ProteinPyruvate carboxylase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1141 (go to sequence)
- Protein existencePredicted
- Annotation score3/5
Function
function
Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Catalytic activity
- N6-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP = N6-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H+This reaction proceeds in the forward direction.
Cofactor
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 131 | ATP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 214 | ATP (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 249 | ATP (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Active site | 306 | |||||
Sequence: R | ||||||
Binding site | 546 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 618 | substrate | ||||
Sequence: R | ||||||
Binding site | 716 | Mn2+ (UniProtKB | ChEBI); via carbamate group | ||||
Sequence: K | ||||||
Binding site | 745 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 747 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 880 | substrate | ||||
Sequence: T |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | biotin carboxylase activity | |
Molecular Function | metal ion binding | |
Molecular Function | pyruvate carboxylase activity | |
Biological Process | gluconeogenesis | |
Biological Process | pyruvate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyruvate carboxylase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Corynebacteriaceae > Corynebacterium
Accessions
- Primary accessionD9QEP1
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Modified residue | 716 | N6-carboxylysine | ||||
Sequence: K | ||||||
Modified residue | 1107 | N6-biotinyllysine | ||||
Sequence: K |
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 15-463 | Biotin carboxylation | ||||
Sequence: SFSKILVANRGEIAVRAFRAAFETGASTVAVYPMEDRNSFHRSFASEAVLIGEGGSAVKAYLDIDEVIRAAKQTGADAVYPGYGFLSENARLARECAENGLTFIGPPPSVLDLTGDKSAAVAAAREAGLPTLTETEPTDDPKELAILSKDQVYPLFVKAVAGGGGRGMRFVEKPEDLEKLAAEASREAAAAFGDGRVYAERAVINPQHIEVQILGDAEGNIVHLYERDCSLQRRHQKVVEIAPAQHLDPDLRDKICADAVAFARHIGYMGAGTVEFLVDEDGNHVFIEMNPRIQVEHTVTEEVTQVDLVKSQIMIAAGATLEQLGLRQEDIHTEGAALQCRITTEDPNNGFRPDTGTITAYRSPGGAGVRLDGAAMLGGEISPNFDSMLVKMTCRGADFETAVARAQRALAEFVVSGVATNIGFLRALLREEDFQSKRIATGFIADHPW | ||||||
Domain | 135-331 | ATP-grasp | ||||
Sequence: VAAAREAGLPTLTETEPTDDPKELAILSKDQVYPLFVKAVAGGGGRGMRFVEKPEDLEKLAAEASREAAAAFGDGRVYAERAVINPQHIEVQILGDAEGNIVHLYERDCSLQRRHQKVVEIAPAQHLDPDLRDKICADAVAFARHIGYMGAGTVEFLVDEDGNHVFIEMNPRIQVEHTVTEEVTQVDLVKSQIMIAA | ||||||
Domain | 537-806 | Pyruvate carboxyltransferase | ||||
Sequence: LAVTDTTFRDAHQSLLATRVRSNTLIDAARHVARLTPELLSVEAWGGATYDVAMRFLHEDPWERLDHLREAMPNINIQMLLRGRNTVGYTPYPDSVCAAFVNEAARSGVDVFRIFDALNDVSQMRPAIDAVLNTGTTVAEVAMAYSGDLTNPKEKLYTLDYYLNLAEQIVDSGAHILAVKDMAGLMKPAAATKLVTELRKNFDLPVHVHTHDTAGGQLATYWAAAAAGADAVDGASAPLSGTTSQPSLSAIVAAFDNTYRSTGLSLDAVG | ||||||
Domain | 1064-1141 | Lipoyl-binding | ||||
Sequence: SAEKADTANVGHVAAPFSGVVTVTAEPGANVAAGDPVAVIEAMKMEATISATKDGTIERIVMTQPTKVEGGDLLLVIS |
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,141
- Mass (Da)122,684
- Last updated2010-10-05 v1
- Checksum072670CDCBB69E44
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP001829 EMBL· GenBank· DDBJ | ADL09964.1 EMBL· GenBank· DDBJ | Genomic DNA |