A9FGB8 · RPOA_SORC5
- ProteinDNA-directed RNA polymerase subunit alpha
- GenerpoA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids346 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity
- RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | DNA-directed RNA polymerase complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | protein dimerization activity | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit alpha
- EC number
- Short namesRNAP subunit alpha
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Myxococcota > Polyangia > Polyangiales > Polyangiaceae > Sorangium
Accessions
- Primary accessionA9FGB8
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000091967 | 1-346 | DNA-directed RNA polymerase subunit alpha | |||
Sequence: MTMNNPNLTMIARNWRDLIRPKGISIDAESGTQFYAKFTCEPLERGFGITIGNSLRRVLLSSLQGAAATAIRIEGALHEFTTVPDVVEDVSDIILNVKEVVFKAATPKTYSVRIDREGPGPVYARDIQLVEGLSVLNPDHLIAVLDKKGPLSMELTVNVGRGYVPAERNKTPTMPIGTIPIDALFSPIRKVNYTVQNARVGQVTDYDKLTLEVWTNGSVSPADAVAFAAKILKEQLSIWVNFEESEETSYQAVMSDDEPLNENLFRSVEELELSVRSANCLQNANITLIGELVQRTEQDMLKTKNFGRKSLKEIKEILANMGLSLGMKIDNWPQLLERWKAQQAQA |
Interaction
Subunit
Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-243 | Alpha N-terminal domain (alpha-NTD) | ||||
Sequence: MTMNNPNLTMIARNWRDLIRPKGISIDAESGTQFYAKFTCEPLERGFGITIGNSLRRVLLSSLQGAAATAIRIEGALHEFTTVPDVVEDVSDIILNVKEVVFKAATPKTYSVRIDREGPGPVYARDIQLVEGLSVLNPDHLIAVLDKKGPLSMELTVNVGRGYVPAERNKTPTMPIGTIPIDALFSPIRKVNYTVQNARVGQVTDYDKLTLEVWTNGSVSPADAVAFAAKILKEQLSIWVNFE | ||||||
Region | 260-346 | Alpha C-terminal domain (alpha-CTD) | ||||
Sequence: LNENLFRSVEELELSVRSANCLQNANITLIGELVQRTEQDMLKTKNFGRKSLKEIKEILANMGLSLGMKIDNWPQLLERWKAQQAQA |
Domain
The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.
Sequence similarities
Belongs to the RNA polymerase alpha chain family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length346
- Mass (Da)38,476
- Last updated2008-02-05 v1
- ChecksumE878ECB45D16E9AF
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AM746676 EMBL· GenBank· DDBJ | CAN98106.1 EMBL· GenBank· DDBJ | Genomic DNA |