A2SBU4 · A2SBU4_METPP
- ProteinSuccinate--CoA ligase [ADP-forming] subunit alpha
- GenesucD
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids297 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.
Catalytic activity
- succinate + ATP + CoA = succinyl-CoA + ADP + phosphate
- GTP + succinate + CoA = succinyl-CoA + GDP + phosphate
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 17-20 | CoA (UniProtKB | ChEBI) | ||||
Sequence: TGKT | ||||||
Binding site | 46 | CoA (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 99-101 | CoA (UniProtKB | ChEBI) | ||||
Sequence: ITE | ||||||
Binding site | 168 | substrate; ligand shared with subunit beta | ||||
Sequence: Y | ||||||
Active site | 256 | Tele-phosphohistidine intermediate | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | succinate-CoA ligase complex (ADP-forming) | |
Molecular Function | nucleotide binding | |
Molecular Function | succinate-CoA ligase (ADP-forming) activity | |
Molecular Function | succinate-CoA ligase (GDP-forming) activity | |
Biological Process | tricarboxylic acid cycle |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSuccinate--CoA ligase [ADP-forming] subunit alpha
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Sphaerotilaceae > Methylibium
Accessions
- Primary accessionA2SBU4
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Subunit
Heterotetramer of two alpha and two beta subunits.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 4-103 | CoA-binding | ||||
Sequence: LINKNTKVITQGITGKTGQFHTRGCRDYANGKNCFVAGVNPKKAGEDFEGIPIYASVKEAAGQTGATVSVIYVPPAGAAAAIWEAVEADLDLAICITEGI |
Sequence similarities
Belongs to the succinate/malate CoA ligase alpha subunit family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length297
- Mass (Da)30,828
- Last updated2007-03-06 v1
- ChecksumFCC9779CA38E23F1
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000555 EMBL· GenBank· DDBJ | ABM93033.1 EMBL· GenBank· DDBJ | Genomic DNA |