A1RT66 · A1RT66_PYRIL

Function

function

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site177-178ATP (UniProtKB | ChEBI)
Binding site258ATP (UniProtKB | ChEBI)
Binding site280ATP (UniProtKB | ChEBI)
Binding site289ATP (UniProtKB | ChEBI)
Active site445Cysteine persulfide intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular FunctionCCA tRNA nucleotidyltransferase activity
Molecular FunctiontRNA binding
Molecular FunctiontRNA-uracil-4 sulfurtransferase activity
Biological Processthiamine biosynthetic process
Biological Processthiamine diphosphate biosynthetic process
Biological ProcesstRNA thio-modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA sulfurtransferase
  • EC number
  • Alternative names
    • Sulfur carrier protein ThiS sulfurtransferase
    • Thiamine biosynthesis protein ThiI
    • tRNA 4-thiouridine synthase

Gene names

    • Name
      thiI
    • Ordered locus names
      Pisl_0972

Organism names

Accessions

  • Primary accession
    A1RT66

Proteomes

Subcellular Location

Keywords

PTM/Processing

Keywords

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain57-159THUMP
Domain397-471Rhodanese

Sequence similarities

Belongs to the ThiI family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    472
  • Mass (Da)
    52,597
  • Last updated
    2007-02-06 v1
  • Checksum
    D6A85914F491D9AF
MERVLIVRVGELTIKRGKTRVEMERLLLRAAKEAATECGNVRFVREPGRIYALGDIDCLRNKLSRVFGVKSVSPAYVIIFEKIEDIVDAALKLWGSAVAGRKFAVRVHRVGEHSFTSRDIAIAVGAVLTKAGGKVDLENPEVELFIEVRNNRAFLYTEVIEGPGGLPIGSEGKVLALVSGGIDSPVAAWMMMRRGAHVDVFYCNLGGTFVARLVVEVIKRLLSWSYGYNARVVITDCAPIGRTIRRNVKEELWNIAFKRALYLTARKVADIVRATALVTGESLGQVSSQTLQALAAVERGLDIPVMRPLVGMDKDEIIQLARKIGTYELSIKIPEYCAIFSKRPKKWATREEIEEIDLAIYDAVIEVVKNIKIVKKRELDSYIASLSPPQDIEIESLPPDAVLIDLRDQKSFQKWHLPGALRADPDDVLTLVDRLGHDKTYVFYCYSGGLSLDVAESLRKFGVKAYSLKLRK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000504
EMBL· GenBank· DDBJ
ABL88148.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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