A0A843IXJ2 · A0A843IXJ2_9EURY
- ProteindITP/XTP pyrophosphatase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids183 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.
Catalytic activity
- H2O + ITP = diphosphate + H+ + IMP
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 8-13 | substrate | ||||
Sequence: TGNENK | ||||||
Binding site | 36 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Active site | 65 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 65 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 66 | substrate | ||||
Sequence: T | ||||||
Binding site | 140-143 | substrate | ||||
Sequence: FGYD | ||||||
Binding site | 161 | substrate | ||||
Sequence: K | ||||||
Binding site | 166-167 | substrate | ||||
Sequence: HR |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | metal ion binding | |
Molecular Function | nucleoside triphosphate diphosphatase activity | |
Molecular Function | nucleotide binding | |
Molecular Function | ribonucleoside triphosphate phosphatase activity | |
Biological Process | nucleotide metabolic process | |
Biological Process | purine nucleoside triphosphate catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namedITP/XTP pyrophosphatase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Stenosarchaea group > Methanomicrobia > Methanomicrobiales > Methanomicrobiaceae > Methanomicrobium
Accessions
- Primary accessionA0A843IXJ2
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length183
- Mass (Da)20,248
- Last updated2021-09-29 v1
- Checksum24C42015645069EA