A0A7Z3CA98 · A0A7Z3CA98_PSEFL
- ProteinEpoxyqueuosine reductase
- GenequeG
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids366 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the conversion of epoxyqueuosine (oQ) to queuosine (Q), which is a hypermodified base found in the wobble positions of tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr).
Catalytic activity
- epoxyqueuosine34 in tRNA + AH2 = queuosine34 in tRNA + A + H2O
Cofactor
Protein has several cofactor binding sites:
Note: Binds 2 [4Fe-4S] clusters per monomer.
Pathway
tRNA modification; tRNA-queuosine biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 63 | cob(II)alamin (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Active site | 140 | Proton donor | ||||
Sequence: D | ||||||
Binding site | 140 | cob(II)alamin (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 161 | cob(II)alamin (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 164 | cob(II)alamin (UniProtKB | ChEBI) | ||||
Sequence: V | ||||||
Binding site | 175 | cob(II)alamin (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Binding site | 195 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 198 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 201 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 205 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 221 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 223 | cob(II)alamin (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 230 | tRNA (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 248 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 248-249 | cob(II)alamin (UniProtKB | ChEBI) | ||||
Sequence: CD | ||||||
Binding site | 251 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 255 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | cobalamin binding | |
Molecular Function | epoxyqueuosine reductase activity | |
Molecular Function | metal ion binding | |
Biological Process | queuosine biosynthetic process | |
Biological Process | tRNA modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameEpoxyqueuosine reductase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas
Accessions
- Primary accessionA0A7Z3CA98
Proteomes
Subcellular Location
Interaction
Subunit
Monomer.
Structure
Sequence
- Sequence statusComplete
- Length366
- Mass (Da)41,123
- Last updated2021-06-02 v1
- Checksum5CB482E9ADCC808A