A0A7J6D205 · A0A7J6D205_9TELE

  • Protein
    NADPH-dependent diflavin oxidoreductase 1
  • Gene
    NDOR1
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

NADPH-dependent reductase which is a central component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Transfers electrons from NADPH via its FAD and FMN prosthetic groups to the [2Fe-2S] cluster of CIAPIN1, another key component of the CIA machinery. In turn, this reduced cluster provides electrons for assembly of cytosolic iron-sulfur cluster proteins. It can also reduce the [2Fe-2S] cluster of CISD1 and activate this protein implicated in Fe/S cluster repair.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
FAD (UniProtKB | Rhea| CHEBI:57692 )

FMN (UniProtKB | Rhea| CHEBI:58210 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site12-17FMN (UniProtKB | ChEBI)
Binding site59-62FMN (UniProtKB | ChEBI)
Binding site97-106FMN (UniProtKB | ChEBI)
Binding site132FMN (UniProtKB | ChEBI)
Binding site349FAD (UniProtKB | ChEBI)
Binding site381-384FAD (UniProtKB | ChEBI)
Binding site415-418FAD (UniProtKB | ChEBI)
Binding site459NADP+ (UniProtKB | ChEBI)
Binding site514-515NADP+ (UniProtKB | ChEBI)
Binding site520-524NADP+ (UniProtKB | ChEBI)
Binding site595FAD (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentperinuclear region of cytoplasm
Molecular Functionflavin adenine dinucleotide binding
Molecular FunctionFMN binding
Molecular FunctionNADP binding
Molecular FunctionNADPH-hemoprotein reductase activity
Biological Processiron-sulfur cluster assembly

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NADPH-dependent diflavin oxidoreductase 1
  • EC number
  • Alternative names
    • NADPH-dependent FMN and FAD-containing oxidoreductase

Gene names

    • Name
      NDOR1
    • ORF names
      G5714_005785

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • SWU-2019
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Cyprinidae > Acrossocheilinae > Onychostoma

Accessions

  • Primary accession
    A0A7J6D205

Proteomes

Subcellular Location

Cytoplasm, perinuclear region
Note: Concentrated in perinuclear structure.

Keywords

Interaction

Subunit

Interacts with CIAPIN1; as part of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain6-150Flavodoxin-like
Domain204-445FAD-binding FR-type

Sequence similarities

Belongs to the NADPH-dependent diflavin oxidoreductase NDOR1 family.
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
In the N-terminal section; belongs to the flavodoxin family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    596
  • Mass (Da)
    67,347
  • Last updated
    2021-04-07 v1
  • Checksum
    699EB91A39F98BDA
MSTHALLVLYGSQTGTAQDTAERIGRQAQRRRMRVRVEALDSYNVANLISESLVVCVCATTGQGDPPDNMKKFWRFLFRKSLPADSLCRLDCAVLGLGDSSYPKFNFIAKKLHKRLLQLGTNMLLPVGLADDQHDLGPDGVIDPWLISFWQKTLSLYPPPAGLAPLREEEKLPPRYVFHFLDEVPDKQTEHLQTPDNKAPPTPLQPFPARVVFNQRVTHPSHFQDVRHIEFDITGSNIQYSAGDVVMMRPCNDAEDVEQFCQLLKLTDPETYFTLTPTDSSTAVPERLPQPCSVRFLVEQFLDISAVPRRSFFEVLATFATNELERDKLLEFSSAQGQDALHSYCNRPRRTALEVLADFPHTTAELSIDYLLDLFPEIQPRSFSIASSLLQQPNRIQILVAVVKYKSLLFKPRKGLCSSWLASLDPSNGDVYVPLWVKKGSLSFPQDPDTPVIMVGPGTGVAPFRSAIQERAAQGKMANVLFFGCRSESKDFYCRSEWEEKVQAGQMILVTAFSRDQEEKIYVQQRVKEQSKLLWDLIAKKNAFFYIAGNAKQMPTSVCDALKEAFQKEGGMSENQAQEMLDAMEKTKRFQSETWS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JAAMOB010000005
EMBL· GenBank· DDBJ
KAF4113240.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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