A0A640W3M1 · A0A640W3M1_UNCTM
- ProteintRNA-t(6)A37 methylthiotransferase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids414 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t6A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms2t6A) at position 37 in tRNAs that read codons beginning with adenine.
Catalytic activity
- N6-L-threonylcarbamoyladenosine37 in tRNA + [sulfur carrier]-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N6-L-threonylcarbamoyladenosine37 in tRNA + [sulfur carrier]-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H+
Cofactor
Note: Binds 1 or 2 [4Fe-4S] cluster. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | metal ion binding | |
Molecular Function | N6-threonylcarbomyladenosine methylthiotransferase activity | |
Molecular Function | tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-t(6)A37 methylthiotransferase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageArchaea > Candidatus Thermoplasmatota > Thermoplasmata
Accessions
- Primary accessionA0A640W3M1
Proteomes
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-111 | MTTase N-terminal | ||||
Sequence: MKVYIETYGCAAAQGDAAIMEGILLQRGYEIVGEPEKADFVVIVTCIVVDTTQQRMISRIKKFKEMGKKIVVAECMASALPDLVKKLDENAILLPPRYIHHIADAIEGKRD | ||||||
Domain | 122-351 | Radical SAM core | ||||
Sequence: RKIGIRLNIPIAEGCIYNCSYCITKLARGRLTSFPLEGLVKDAENAIKKGCKELRLTTQDTASYGIDIGKSLPELINEIASIEGEFRIRIGMMHPLSAYRIFDELMEAYENEKVYKFIHLPLQSASDKILRAMRRGYTYDLFKEMVDEFRKRFRHSTFATDIIVAFPGEGEEEFEETMKAMKELRPDITNVTRFSPRPFTEAAKLKKLPTQVAKQRSRAASELALKISYE | ||||||
Domain | 354-412 | TRAM | ||||
Sequence: RELVGKTFRALLLEERNGWIVGKTDGYRSVYVKNAAIGEFVKVAIKKAKLTHLEGEITP |
Sequence similarities
Belongs to the methylthiotransferase family. CDKAL1 subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length414
- Mass (Da)46,976
- Last updated2020-04-22 v1
- Checksum7C0C7907EDE41EB1