A0A4Q8R9F6 · A0A4Q8R9F6_9BRAD
- ProteinThiamine pyrimidine synthase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1060 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Responsible for the formation of the pyrimidine heterocycle in the thiamine biosynthesis pathway. Catalyzes the formation of hydroxymethylpyrimidine phosphate (HMP-P) from histidine and pyridoxal phosphate (PLP). The protein uses PLP and the active site histidine to form HMP-P, generating an inactive enzyme. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme.
Catalytic activity
- N6-(pyridoxal phosphate)-L-lysyl-[4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase] + L-histidyl-[4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase] + 2 Fe3+ + 4 H2O = L-lysyl-[4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase] + (2S)-2-amino-5-hydroxy-4-oxopentanoyl-[4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase] + 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 3-oxopropanoate + 2 Fe2+ + 2 H+This reaction proceeds in the forward direction.
Pathway
Cofactor biosynthesis; thiamine diphosphate biosynthesis.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Molecular Function | adenylate cyclase activity | |
Molecular Function | metal ion binding | |
Molecular Function | transferase activity | |
Biological Process | cyclic nucleotide biosynthetic process | |
Biological Process | intracellular signal transduction | |
Biological Process | thiamine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameThiamine pyrimidine synthase
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Nitrobacteraceae > Bradyrhizobium
Accessions
- Primary accessionA0A4Q8R9F6
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 342-359 | Helical | ||||
Sequence: VWAFLAVVVAILLGRILY | ||||||
Transmembrane | 371-396 | Helical | ||||
Sequence: LSAAMSGLFVLLSIPALIFILAYNYW | ||||||
Transmembrane | 713-731 | Helical | ||||
Sequence: MVALIVALTGIELLLIYFF |
Keywords
- Cellular component
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 813-945 | Guanylate cyclase | ||||
Sequence: TMLFTDLKDFSTLSEQMAPNDLLAQLSHYFEVVSQAIAEESGTVDKFIGDGIMAFWGAPVHRDDDVLRGCCGAMRATRRMEQLNADWSKQGRPSLHLRVGLHCAEVLVGNVGSSERLSYTVMGDGVNVAARLE |
Sequence similarities
Belongs to the NMT1/THI5 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,060
- Mass (Da)117,240
- Last updated2019-07-31 v1
- Checksum0FB421FE828300D8
Keywords
- Technical term