A0A4Q3ZEP7 · A0A4Q3ZEP7_9PROT

Function

function

Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Pathway

Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.
Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site190Mg2+ 1 (UniProtKB | ChEBI)
Binding site190Mg2+ 2 (UniProtKB | ChEBI)
Binding site194Mg2+ 1 (UniProtKB | ChEBI)
Binding site226Mg2+ 2 (UniProtKB | ChEBI)
Binding site230Mg2+ 2 (UniProtKB | ChEBI)
Binding site251substrate

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionisomerase activity
Molecular Functionketol-acid reductoisomerase activity
Molecular Functionmetal ion binding
Molecular FunctionNADP binding
Biological Processisoleucine biosynthetic process
Biological Processvaline biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ketol-acid reductoisomerase
  • EC number

Gene names

    • Name
      ilvC
    • ORF names
      EON57_06205

Organism names

Accessions

  • Primary accession
    A0A4Q3ZEP7

Proteomes

Subcellular Location

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain1-181KARI N-terminal Rossmann
Domain182-312KARI C-terminal knotted

Sequence similarities

Belongs to the ketol-acid reductoisomerase family.

Family and domain databases

Sequence

  • Sequence status
    Fragment
  • Length
    312
  • Mass (Da)
    33,691
  • Last updated
    2019-07-31 v1
  • Checksum
    4665B386E3138184
MKVYYDKDCDLSLIKGKQVTIVGYGSQGHAHAQNLNDSGVKVTVGLRKGGASWAKAEGAGLAVKEVADAVKSADVVMILLPDEQIANVYKKDVEPNIKQGASLVFAHGFNVHYGFVQPRADLDVWMVAPKAPGHTVRGTYTQGGGVPHLVAVHQDKTGKARDLALSYSMANGGGKAGIIETNFREETETDLFGEQAVLCGGTVELIKAGFETLVEAGYAPEMAYFECLHELKLIVDLIYEGGIANMNYSISNNAEYGEYVTGPRVVTEETKKVMKQVLRDIQTGEYAKSFVLEAAAGQPALISRRRLNAEHQ

Features

Showing features for non-terminal residue.

TypeIDPosition(s)Description
Non-terminal residue312

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
SDYG01000399
EMBL· GenBank· DDBJ
RYH07058.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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