A0A4M5PYF9 · A0A4M5PYF9_STREE
- ProteinUDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase GtfA subunit
- GenetagE
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids503 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Required for polymorphic O-glycosylation of the serine-rich repeat protein in this bacteria. Catalyzes the first step in glycosylation by transferring N-acetylglucosamine from UDP-GlcNAc to serine residues in the substrate protein. Part of the accessory SecA2/SecY2 system specifically required to export serine-rich repeat cell wall proteins usually encoded upstream in the same operon.
Catalytic activity
- L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-[N-acetyl-alpha-D-glucosaminyl]-L-seryl-[protein] + H+ + UDP
Pathway
Protein modification; protein glycosylation.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | plasma membrane | |
Cellular Component | protein N-acetylglucosaminyltransferase complex | |
Molecular Function | glycosyltransferase activity | |
Molecular Function | nucleotide binding | |
Biological Process | protein O-linked glycosylation via serine |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameUDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase GtfA subunit
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Lactobacillales > Streptococcaceae > Streptococcus
Accessions
- Primary accessionA0A4M5PYF9
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Peripheral membrane protein
Note: Cell membrane association requires GtfB.
Keywords
- Cellular component
Interaction
Subunit
Forms a heterotetramer with 2 subunits each of GtfA and GtfB. Part of the accessory SecA2/SecY2 protein translocation apparatus.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 95-191 | GtfA extended beta-sheet meander | ||||
Sequence: REKNGKVLRVFFFDQDKFVTCYLVDENKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAVLYQRTFYNEDGTPVYDILMNQGKEEVYHFKD |
Sequence similarities
Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length503
- Mass (Da)57,767
- Last updated2019-09-18 v1
- Checksum821F3A4F37DA3164