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Entry version 8 (31 Jul 2019)
Sequence version 1 (07 Nov 2018)
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Protein

Multifunctional fusion protein

Gene

cbiD

Organism
Spirochaetia bacterium
Status
Unreviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the ATP-dependent amidation of the two carboxylate groups at positions a and c of cobyrinate, using either L-glutamine or ammonia as the nitrogen source.UniRule annotation
Catalyzes the methylation of C-1 in cobalt-precorrin-5B to form cobalt-precorrin-6A.UniRule annotation

Miscellaneous

The a and c carboxylates of cobyrinate are activated for nucleophilic attack via formation of a phosphorylated intermediate by ATP. CbiA catalyzes first the amidation of the c-carboxylate, and then that of the a-carboxylate.UniRule annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+UniRule annotation

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 10 and 6 of the subpathway that synthesizes cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route).UniRule annotation
Proteins known to be involved in the 10 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
  6. Multifunctional fusion protein (cbiD)
  7. no protein annotated in this organism
  8. no protein annotated in this organism
  9. no protein annotated in this organism
  10. Multifunctional fusion protein (cbiD)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route), the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei363NucleophileUniRule annotation1
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections (‘Function’, ‘PTM / Processing’, ‘Pathology and Biotech’) according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei477Increases nucleophilicity of active site CysUniRule annotation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionLigaseUniRule annotation, MethyltransferaseUniRule annotation, Transferase
Biological processCobalamin biosynthesisUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Nucleotide-binding, S-adenosyl-L-methionineUniRule annotation

Enzyme and pathway databases

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00148;UER00227
UPA00148;UER00231

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Multifunctional fusion proteinUniRule annotation
Including the following 2 domains:
Cobyrinate a,c-diamide synthaseUniRule annotation (EC:6.3.5.11UniRule annotation)
Alternative name(s):
Cobyrinic acid a,c-diamide synthetaseUniRule annotation
Cobalt-precorrin-5B C(1)-methyltransferaseUniRule annotation (EC:2.1.1.195UniRule annotation)
Alternative name(s):
Cobalt-precorrin-6A synthaseUniRule annotation
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:cbiDUniRule annotation
Synonyms:cbiAUniRule annotation
ORF Names:DC049_07330Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSpirochaetia bacteriumImported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri2053615 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaSpirochaetes

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini280 – 485GATase cobBQ-typeInterPro annotationAdd BLAST206

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1107 – 1126DisorderedSequence analysisAdd BLAST20

<p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

Comprises of two domains. The C-terminal domain contains the binding site for glutamine and catalyzes the hydrolysis of this substrate to glutamate and ammonia. The N-terminal domain is anticipated to bind ATP and cobyrinate and catalyzes the ultimate synthesis of the diamide product. The ammonia produced via the glutaminase domain is probably translocated to the adjacent domain via a molecular tunnel, where it reacts with an activated intermediate.UniRule annotation

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the CbiD family.UniRule annotation
Belongs to the CobB/CbiA family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferaseUniRule annotationSAAS annotation

Family and domain databases

Conserved Domains Database

More...
CDDi
cd11644 Precorrin-6Y-methylase, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.30.2110.10, 1 hit
3.40.1010.10, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_00787 CbiD, 1 hit
MF_00027 CobB_CbiA, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR000878 4pyrrol_Mease
IPR035996 4pyrrol_Methylase_sf
IPR014777 4pyrrole_Mease_sub1
IPR004484 CbiA_synth
IPR002748 CbiD
IPR036074 CbiD_sf
IPR012818 Cbl_synth_CbiE
IPR029062 Class_I_gatase-like
IPR017929 CobB/CobQ_GATase
IPR002586 CobQ/CobB/MinD/ParA_Nub-bd_dom
IPR011698 GATase_3
IPR027417 P-loop_NTPase

The PANTHER Classification System

More...
PANTHERi
PTHR43873 PTHR43873, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01656 CbiA, 1 hit
PF01888 CbiD, 1 hit
PF07685 GATase_3, 1 hit
PF00590 TP_methylase, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF111342 SSF111342, 1 hit
SSF52317 SSF52317, 1 hit
SSF52540 SSF52540, 1 hit
SSF53790 SSF53790, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00312 cbiD, 1 hit
TIGR02467 CbiE, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51274 GATASE_COBBQ, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

A0A352VXM4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKPGVILVAG TGSGVGKTSI AVGLARALAD RSFTVQPFKT GPDFLDPVWL
60 70 80 90 100
TRAARRSCVS LDGWMCGKSY VCSLAETLSR DADIILVEGV MGMYDGADPG
110 120 130 140 150
NLAGSSAEIA VWLDASVLLV INVHGSVRST AAIARGFADF EPAPGVAMVI
160 170 180 190 200
ANQAGSSSHA DMLATAFAAP GCPPLAGVIP RGALSSLSSR HLGLAAPDHD
210 220 230 240 250
DRGASAAEET IGALASAVEK HIQIDLILSR INIKQDKAVK KINIPPSPSR
260 270 280 290 300
YYSYALGSAP SLLYAPYDKL FLKKPDHNVK IGIALDEAFS FYYPDNLEAI
310 320 330 340 350
KEAGAELVPF SPLHDNCLPE NLDGIYLGGG YPELHAEKLS ENTGIKNAIT
360 370 380 390 400
AFAGTGHTVY AECGGIMYLS RGITTVSGNF FLMCGILPFA VRMSSSLRRL
410 420 430 440 450
GYVSIKTEAD CIWGKAGTEI RGHEFHYSEI DRQNGQPDFT DCSRQGWTRI
460 470 480 490 500
YSAEHTRTGR REPEGFYNGC VLASYVHLHF ASQPACARSF VEFCARKKSL
510 520 530 540 550
KIPPVSSSEN IITLRSGFTT GSAAAAAALA AAVLIAGRKA LNRAEIIIPG
560 570 580 590 600
KSENTGESGI LAIPVAESNL IRPGAAASAV VIKDAGDDPD ITDRVHIRVL
610 620 630 640 650
VEHTEEPGRN ILFQAGEGVG IVTKPGLQIP PGEPAINPVP RAMITDALRS
660 670 680 690 700
VLPEGSWRIT VSAAGGENLA LKTFNPRLGI TGGISILGTT GIVRPFSHSA
710 720 730 740 750
LKSALRCAFN VALASGADPL VLVPGNIGRR AFSAAYPEFA DDHIIDVSNE
760 770 780 790 800
WGTVLEWIHE AKTIKTIAVC GHPGKLTKLL GDSWDTHSSR SPSALPLIFS
810 820 830 840 850
LAREAGINYS FDNITTAEGF FTDIKSDERE KIANLLAQRI CDKIKIRCFC
860 870 880 890 900
ADAAVLLCDM NGKIIGSTGS DIFKRKYSTT AEVSAGGRVS HIKDETRKIV
910 920 930 940 950
IAGCGPGSHN HLTAEVIEAV KNADVLIGTD RVLELFSESK ARKITCNGSI
960 970 980 990 1000
NAVCDIITRE DAHRVCVLVT GDPGFYSLAS LVVRRFGARQ CIILPGITSV
1010 1020 1030 1040 1050
QAAAARLGIS WAHALLLSAH TKIPQDTEIF VNDRELPETL IILGGCKDSP
1060 1070 1080 1090 1100
EWITRLGQRL GSKYRGWLCE NICLPDERIT AFPGVPQSGS VWLVIFSRCA
1110 1120
YNQDVEMSPD TKSSQKESXQ AMKQMG
Length:1,126
Mass (Da):120,197
Last modified:November 7, 2018 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i080D983BC8628366
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
DNNS01000275 Genomic DNA Translation: HBE02273.1

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DNNS01000275 Genomic DNA Translation: HBE02273.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

Enzyme and pathway databases

UniPathwayiUPA00148;UER00227
UPA00148;UER00231

Family and domain databases

CDDicd11644 Precorrin-6Y-methylase, 1 hit
Gene3Di3.30.2110.10, 1 hit
3.40.1010.10, 1 hit
HAMAPiMF_00787 CbiD, 1 hit
MF_00027 CobB_CbiA, 1 hit
InterProiView protein in InterPro
IPR000878 4pyrrol_Mease
IPR035996 4pyrrol_Methylase_sf
IPR014777 4pyrrole_Mease_sub1
IPR004484 CbiA_synth
IPR002748 CbiD
IPR036074 CbiD_sf
IPR012818 Cbl_synth_CbiE
IPR029062 Class_I_gatase-like
IPR017929 CobB/CobQ_GATase
IPR002586 CobQ/CobB/MinD/ParA_Nub-bd_dom
IPR011698 GATase_3
IPR027417 P-loop_NTPase
PANTHERiPTHR43873 PTHR43873, 1 hit
PfamiView protein in Pfam
PF01656 CbiA, 1 hit
PF01888 CbiD, 1 hit
PF07685 GATase_3, 1 hit
PF00590 TP_methylase, 1 hit
SUPFAMiSSF111342 SSF111342, 1 hit
SSF52317 SSF52317, 1 hit
SSF52540 SSF52540, 1 hit
SSF53790 SSF53790, 1 hit
TIGRFAMsiTIGR00312 cbiD, 1 hit
TIGR02467 CbiE, 1 hit
PROSITEiView protein in PROSITE
PS51274 GATASE_COBBQ, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiA0A352VXM4_9SPIR
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: A0A352VXM4
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/TrEMBL: November 7, 2018
Last sequence update: November 7, 2018
Last modified: July 31, 2019
This is version 8 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiUnreviewed (UniProtKB/TrEMBL)
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