A0A317VG17 · A0A317VG17_ASPEC

Function

function

NADH-dependent reductase for DPH3 and cytochrome b5. Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase, predominantly CBR1. By reducing DPH3, also involved in the formation of the tRNA wobble base modification mcm5s 2U (5-methoxycarbonylmethyl-2-thiouridine), mediated by the elongator complex. The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

FAD (UniProtKB | Rhea| CHEBI:57692 )

Pathway

Protein modification; peptidyl-diphthamide biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site110FAD (UniProtKB | ChEBI)
Binding site112FAD (UniProtKB | ChEBI)
Binding site127FAD (UniProtKB | ChEBI)
Binding site129FAD (UniProtKB | ChEBI)
Binding site136FAD (UniProtKB | ChEBI)
Binding site137FAD (UniProtKB | ChEBI)
Binding site178FAD (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum
Cellular Componentmitochondrial outer membrane
Molecular Functioncytochrome-b5 reductase activity, acting on NAD(P)H
Molecular Functiontransferase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NADH-cytochrome b5 reductase
  • EC number

Gene names

    • ORF names
      BO83DRAFT_361881

Organism names

Accessions

  • Primary accession
    A0A317VG17

Proteomes

Organism-specific databases

Subcellular Location

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain58-161FAD-binding FR-type

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    305
  • Mass (Da)
    33,486
  • Last updated
    2018-10-10 v1
  • Checksum
    C013EECF90582E44
MSAFSPENITGAFIPSALFVAGTFLFKQELTPFAVALAAILVGWKVFSNKPRKVLNPGDFQHFTLKEKNDISHNVTVYRFALPRPTDILGLPIGQHISLAATIGGKEVVRSYTPISSDNEAGYFDLLVKAYPQGNISKYLTTLEVGQTMKVRGPKGAMVYTPNMCRHIGMIAGGTGITPMYQIIKAIIRNRPRNGGNDTTQVDLIFANVNPDDILMKEELEQLAKEDDGFRIYYVLNNPPEGWTGGVGFVTPDMIKERLPAPASDIKILLCGPPPMVSAMKKATESLGYTKARPVSKLEDQIFCF

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MSFU01000014
EMBL· GenBank· DDBJ
PWY71862.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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